| Project/Area Number |
24592719
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Plastic surgery
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| Research Institution | Keio University |
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Principal Investigator |
HAYASHI RUKA 慶應義塾大学, 医学部, 助教 (50445392)
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| Co-Investigator(Kenkyū-buntansha) |
TORIUMI Masahiro 帝京大学, 医学部, 助教 (20528210)
KISHI Kazuo 慶應義塾大学, 医学部, 教授 (40224919)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 細胞凝集塊 / 非接着培養 / 間葉系細胞 / 線維芽細胞 / 毛包誘導 / 幹細胞 / 免疫組織学的検討 / 遺伝子学的検討 / 再生医療 / 未分化 / 三次元培養 / 未分化性 |
|---|
| Outline of Final Research Achievements |
Mesenchymal cells were extracted and cultured from skin of newborn C57BL/6J mice, and each cell spheres were made using serum-free medium on non-adhesive culture dishes. Spheres were viable for the comparative long term of the months unit in spite of the non-adhesive condition, which suggest the possibility that the spheres were in so-called hibernation state. In genetic and immunohistological examination, the Wnt related markers which supposed to concerned with hair follicle regeneration and hair cycle, and also the undifferentiated markers such as Sox-2, Oct-4, CD133, cxcr4 were seen to relatively upregulated after forming the cell spheres. But the distribution of the markers was not equal in cell spheres, it is necessary to examine whether it is the result of character change in some specific cells or the result of the cell choice.
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