| Project/Area Number |
24592721
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Plastic surgery
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| Research Institution | Nihon University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
MATSUMOTO Taro 日本大学, 医学部, 教授 (50366580)
KASHIMURA Tsutomu 日本大学, 医学部, 助教 (20570740)
NAKAZAWA Hiroaki 日本大学, 医学部, 教授 (60180270)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 人工真皮 / 培養表皮 / 脱分化脂肪細胞 / 皮膚 / 脱分化脂肪 |
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| Outline of Final Research Achievements |
Our group has established an adipocyte progenitor cell line from mature adipocytes and named them dedifferentiated fat (DFAT) cells. DFAT cells have been shown to have multilineage differentiation potential similar to that of adipose tissue-derived stem cells. This study aimed to investigate the effects of DFAT cells on novel skin reconstruction methods.
With combination therapy of bFGF and DFAT cells, skin graft revascularization was markedly promoted. Combined use of DFAT cells and bFGF markedly promoted dermis-like tissue generation and vascularization, and capillary infiltration into the dermis was obtained within 2 days after application of AD , which quite strongly suggests the feasibility of one-step grafting. In cases of autologous cultured epithelial grafting, treatment with DFAT enhanced laminin formation at the epidermal-dermal junction and regeneration of basement membranes.
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