A study of abnormal orofacial pain and neuron-glia interactions in the central nervous system

• Project/Area Number: 24592764
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Morphological basic dentistry
• Research Institution: Okayama University
• Principal Investigator: TERAYAMA RYUJI 岡山大学, 医歯(薬)学総合研究科, 准教授 (60333689)
• Co-Investigator(Renkei-kenkyūsha): SUGIMOTO Tomosada 岡山大学, 大学院医歯薬学総合研究科, 教授 (50135729)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 痛覚異常 / 神経損傷 / 収斂投射 / ミクログリア / 三叉神経知覚核群 / 脊髄 / グリア細胞 / ニューロン

Outline of Final Research Achievements

In this study, involvement of neurons and glial cells in the central nervous system in abnormal orofacial pain sensations after peripheral nerve injury was investigated. Differential changes in neuronal excitability in the brainstem were observed after trigeminal nerve injury. Established an objective method for assessing intraoral pain, I found that injury to the peripheral nerve exaggerated the behavioral responses and that convergent primary nociceptive input through neighboring intact nerves may contribute to the pathogenesis of neuropathic pain following trigeminal nerve injury. Furthermore, I confirmed the relationship between abnormal pain sensations and the convergence in the spinal cord following injury to the nerve innervating the hindpaw and found that microglial activation following peripheral nerve injury plays an important role in the anomalous convergence of nociceptive signals to spinal dorsal horn neurons and the development of neuropathic pain.

Research Products

• [Journal Article] Activated microglia contribute to convergent nociceptive inputs to spinal dorsal horn neurons and the development of neuropathic pain (2015)
  • Author(s): Yamamoto Y, Terayama R, Kishimoto N, Maruhama K, Mizutani M, Iida S, Sugimoto T
  • Journal Title: Neurochem Res Volume: 40
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Peripheral nerve injury activates convergent nociceptive input to dorsal horn neurons from neighboring intact nerve (2015)
  • Author(s): Terayama R, Yamamoto Y, Kishimoto N, Maruhama K, Mizutani M, Iida S, Sugimoto T
  • Journal Title: Exp Brain Res Volume: 233 Issue: 4 Pages: 1201-1212
  • DOI: 10.1007/s00221-015-4203-2
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Convergent nociceptive input to spinal dorsal horn neurons after peripheral nerve injury (2015)
  • Author(s): Terayama R, Kishimoto N, Yamamoto Y, Maruhama K, Tsuchiya H, Mizutani M, Iida S, Sugimoto T
  • Journal Title: Neurochem Res Volume: 40 Issue: 3 Pages: 438-445
  • DOI: 10.1007/s11064-014-1484-y
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Possible involvement of convergent nociceptive input to medullary dorsal horn neurons in intraoral hyperalgesia following peripheral nerve injury (2015)
  • Author(s): Terayama R, Tsuchiya H, Omura S, Maruhama K, Mizutani M, Iida S, Sugimoto T
  • Journal Title: Cell Mol Neurobiol Volume: 35 Issue: 3 Pages: 417-423
  • DOI: 10.1007/s10571-014-0137-7
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Basic research and clinical investigations of the neural basis of orofacial pain (2015)
  • Author(s): Matsuka Y, Iwata K, Terayama R, Imamura Y, Maruhama K, Shinoda M, Tsuboi Y, Kondo M, Honda K, Katagiri A, Sugimoto T
  • Journal Title: J Oral Biosci Volume: 57 Issue: 1 Pages: 27-36
  • DOI: 10.1016/j.job.2014.09.001
  • NAID: 120006493387
  • Peer Reviewed / Open Access
• [Journal Article] Assessment of intraoral mucosal pain induced by the application of capsaicin (2014)
  • Author(s): Terayama R, Maruhama K, Tsuchiya H, Mizutani M, Iida S, Sugimoto T
  • Journal Title: Arch Oral Biol Volume: 59 Issue: 12 Pages: 1334-1341
  • DOI: 10.1016/j.archoralbio.2014.08.008
  • NAID: 120005477122
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Effect of adenosine A1 receptor agonist on the enhanced excitability of spinal dorsal horn neurons after peripheral nerve injury (2014)
  • Author(s): Yamaguchi D, Terayama R, Omura S, Tsuchiya H, Sato T, Ichikawa H, Sugimoto T
  • Journal Title: Int J Neurosci Volume: 124 Issue: 3 Pages: 213-222
  • DOI: 10.3109/00207454.2013.842566
  • Peer Reviewed
• [Journal Article] Fos protein-like immunoreactive neurons induced by electrical stimulation in the trigeminal sensory nuclear complex of rats with chronically injured peripheral nerve (2012)
  • Author(s): Fujisawa N
  • Journal Title: Experimental Brain Research Volume: 219 Issue: 2 Pages: 191-201
  • DOI: 10.1007/s00221-012-3078-8
  • Peer Reviewed
• [Presentation] 下歯槽神経損傷後の延髄後角における収斂投射 (2014)
  • Author(s): 寺山隆司、丸濱功太郎、土屋泰規、大村晋司、杉本朋貞
  • Organizer: 第56回歯科基礎医学会学術大会・総会
  • Place of Presentation: 福岡国際会議場（福岡市）
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] 末梢神経損傷は脊髄後角での近接神経からの収斂投射を促進する (2014)
  • Author(s): 寺山隆司、山本祐也、岸本宣子、丸濵功太郎、水谷雅英、飯田征二、杉本朋貞
  • Organizer: 第37回日本神経科学大会
  • Place of Presentation: パシフィコ横浜（横浜市）
  • Year and Date: 2014-09-11 – 2014-09-13
• [Presentation] 精製A型ボツリヌス毒素の軸索輸送と疼痛抑制効果 (2014)
  • Author(s): 丸濵功太郎、松香芳三、山本由弥子、寺山隆司、杉本朋貞
  • Organizer: 第37回日本神経科学大会
  • Place of Presentation: パシフィコ横浜（横浜市）
  • Year and Date: 2014-09-11 – 2014-09-13
• [Presentation] 口腔顔面の侵害受容機構と痛覚異常 (2013)
  • Author(s): 寺山隆司、杉本朋貞
  • Organizer: 第55回歯科基礎医学会学術大会・総会
  • Place of Presentation: 岡山市
  • Invited
• [Presentation] 一次求心ニューロンによる精製A 型ボツリヌス毒素の取り込みと軸索輸送 (2013)
  • Author(s): 丸濵功太郎、松香芳三、寺山隆司、窪木拓男、杉本朋貞
  • Organizer: 第55回歯科基礎医学会学術大会・総会
  • Place of Presentation: 岡山市
• [Presentation] Possible involvement of convergent nociceptive inputs to the medullary dorsal horn in intraoral hyperalgesia following peripheral nerve injury (2012)
  • Author(s): Terayama R, Takahashi M, Mori K, Yamashita T, Omura S, Tsuchiya H, Sugimoto T
  • Organizer: 第35回日本神経科学大会
  • Place of Presentation: 名古屋市
• [Presentation] 末梢神経損傷後の中枢神経における１次ニューロンの収斂投射の研究 (2012)
  • Author(s): 大村晋司、土屋泰規、寺山隆司、杉本朋貞
  • Organizer: 日本解剖学会第67回中国・四国支部学術集会
  • Place of Presentation: 宇部市
• [Remarks] 岡山大学口腔機能解剖学分野
  • URL: http://www.cc.okayama-u.ac.jp/~dentanatomy2/
• [Remarks] 岡山大学 口腔機能解剖学分野
  • URL: http://www.cc.okayama-u.ac.jp/~dentanatomy2/
• [Remarks] 研究概要
  • URL: http://www.cc.okayama-u.ac.jp/~dentanatomy2/research.html