Infection control with SLPI via the inhibitory effect on the proteases of Porphyromonas gingivalis

• Project/Area Number: 24592775
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Morphological basic dentistry
• Research Institution: Iwate Medical University
• Principal Investigator: KIMURA Shigenobu 岩手医科大学, 歯学部, 教授 (10177917)
• Co-Investigator(Kenkyū-buntansha): NEMOTO Yuko (OHARA Yuko) 長崎大学大学院, 医歯薬学総合研究科, 准教授 (10164667)
• Co-Investigator(Renkei-kenkyūsha): SASAKI Minoru 岩手医科大学, 歯学部, 准教授 (40187133) ; SHIMOYAMA Yu 岩手医科大学, 歯学部, 助教 (90453331) ; ISHIKAWA Taichi 岩手医科大学, 歯学部, 助教 (10569247)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2012: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
• Keywords: SLPI / 感染制御 / P. gingivalis / プロテアーゼ阻害作用 / ぷろテアーゼ阻害作用

Outline of Final Research Achievements

The results indicated that secretory leukocyte protease inhibitor (SLPI) showed a marked inhibitory effect on gingipains (Rgp and Kgp), the major and crucial proteases of Porphyromonas gingivalis, especially at relatively higher pH (8.0 and 8.5). Thus, SLPI could down-regulate the P. gingivalis infection in the gingival crevice through the inhibitory effect on gingipains.

Research Products

• [Journal Article] Identification and characterization of prokaryotic dipeptidyl-peptidase 5 from Porphyromonas gingivalis. (2014)
  • Author(s): Ohara-Nemoto Y, Rouf SMA, Naito M, Yanase A, Tetsuo F, Ono T, Kobayakawa T, Shimoyama Y, Kimura S, Nakayama K, Saiki K, Konishi K, Nemoto TK.
  • Journal Title: J. Biol. Chem. Volume: 289 Issue: 9 Pages: 5438-5448
  • DOI: 10.1074/jbc.m113.527333
  • NAID: 120006986777
  • Peer Reviewed / Open Access
• [Journal Article] Effects of tongue cleaning on bacterial flora in tongue coating and dental plaque: A crossover study. (2014)
  • Author(s): Matsui, M., Chosa, N., Shimoyama, Y., Minami, K., Kimura, S., Kishi, M.
  • Journal Title: BMC Oral Health Volume: 14 Issue: 1 Pages: 4-4
  • DOI: 10.1186/1472-6831-14-4
  • Peer Reviewed
• [Journal Article] Identification of dipeptidyl-peptidase (DPP)5 and DPP7 in Porphyromonas endodontalis, distinct from those in Porphyromonas gingivalis. (2014)
  • Author(s): Nishimata H, Ohara-Nemoto Y, Baba TT, Hoshino T, Fujiwara T, Shimoyama Y, Kimura S, Nemoto TK.
  • Journal Title: PLoS One Volume: 9 Issue: 12 Pages: 1-15
  • DOI: 10.1371/journal.pone.0114221
  • NAID: 120006986295
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Prediction of periodontopathic bacteria in dental plaque of periodontal healthy subjects by measurement of volatile sulfur compounds in mouth air (2013)
  • Author(s): Mitsuo Kishi
  • Journal Title: Archives of Oral Biology Volume: 58(3) Issue: 3 Pages: 324-330
  • DOI: 10.1016/j.archoralbio.2012.09.019
  • NAID: 120006985841
  • Peer Reviewed
• [Journal Article] Phenylalanine 664 of dipeptidyl peptidase (DPP) 7 and Phenylalanine 671 of DPP11 mediate preference for P2-position hydrophobic residues of a substrate. (2013)
  • Author(s): Rouf, S. M., Ohara-Nemoto, Y., Ono, T., Shimoyama, Y., Kimura, S. and Nemoto, T. K.
  • Journal Title: FEBS Open Bio. Volume: 3 Issue: 1 Pages: 177-183
  • DOI: 10.1016/j.fob.2013.03.004
  • NAID: 120006985739
  • Peer Reviewed
• [Journal Article] In vitro processing of glutamyl endopeptidase proenzymes from Enterococcus faecalis and importance of N-terminal residue in enzyme catalysis. (2013)
  • Author(s): Rouf, S. M., Ohara-Nemoto, Y., Ono, T., Shimoyama, Y., Kimura, S. and Nemoto, T. K.
  • Journal Title: Adv. Biochem. Volume: 1 Issue: 5 Pages: 73-80
  • DOI: 10.11648/j.ab.20130105.11
  • Peer Reviewed
• [Journal Article] Pathogenic factors of P. gingivalis and host defense mechanisms. (2012)
  • Author(s): Kimura, S., Ohara-Nemoto, Y., Shimoyama, Y., Ishikawa, T. and Sasaki, M.
  • Journal Title: Pathogenesis and treatment of periodontitis (N. Buduneli, ed.), InTech Volume: 1 Pages: 3-18
  • Peer Reviewed
• [Journal Article] NVS and staphylococci in the oral cavity – A cause of infective endocarditis. (2012)
  • Author(s): Ohara-Nemoto, Y., Kimura, S. and Nemoto, T.K.
  • Journal Title: Endocarditis (Breijo-Marquez, F.R, ed.), InTech Volume: 1 Pages: 75-96
  • Peer Reviewed
• [Journal Article] In vitro adherence of Candida albicans to acrylic resin with different surface status. (2012)
  • Author(s): Nomura, T., Suzuki, T., Furuya, J., Shimoyama, Y., Sasaki, M. and Kimura, S.
  • Journal Title: Interface Oral Health Science 2011 (Sasaki, K. et al., eds.), Springer Japan Volume: 1 Pages: 145-146
  • DOI: 10.1007/978-4-431-54070-0_36
  • ISBN: 9784431540694, 9784431540700
  • Peer Reviewed
• [Journal Article] Maternal transmission of mutans and other oral streptococcal species. (2012)
  • Author(s): Otake-Asakawa, A., Harada-Oikawa, R., Ohara-Nemoto, Y., Tanaka, M. and Kimura, S.
  • Journal Title: Interface Oral Health Science 2011 (Sasaki, K. et al., eds.), Springer Japan Volume: 1 Pages: 198-200
  • Peer Reviewed
• [Journal Article] Fibronectin binding activity of Streptococcus anginosus promotes adherence to mucosal epithelial cells. (2012)
  • Author(s): Sasaki, M., Kodama, Y., Shimoyama, Y., Ishikawa, T. and Kimura, S.
  • Journal Title: Interface Oral Health Science 2011 (Sasaki, K. et al., eds.), Springer Japan Volume: 1 Pages: 204-205
  • Peer Reviewed
• [Journal Article] Rapid identification of Abiotrophia/Granulicatella species by 16S rRNA-based PCR and RFLP. (2012)
  • Author(s): Shimoyama, Y., Sasaki, M., Ohara-Nemoto, Y., Nemoto, T.K., Ishikawa, T. and Kimura, S.
  • Journal Title: Interface Oral Health Science 2011 (Sasaki, K. et al., eds.), Springer Japan Volume: 1 Pages: 206-208
  • Peer Reviewed
• [Journal Article] Macrophage reaction against sub-micron titanium particles. (2012)
  • Author(s): Taira, M., Sasaki, M. and Kimura, S.
  • Journal Title: Interface Oral Health Science 2011 (Sasaki, K. et al., eds.), Springer Japan Volume: 1 Pages: 283-284
  • Peer Reviewed
• [Presentation] Laminine 411 and 421 differentially promote tumor cell migration via alpha6beta1 integrin and MCAM. (2015)
  • Author(s): 石河太知，下山 佑，根本優子，佐々木 実，木村重信
  • Organizer: 第88回日本細菌学会総会
  • Place of Presentation: 岐阜
• [Presentation] SLPIによるPorphyromonas gingivalisに対する感染抑制作用 (2014)
  • Author(s): 下山 佑，石河太知，根本優子，根本孝幸，佐々木 実，木村重信
  • Organizer: 第56回歯科基礎医学会学術大会・総会
  • Place of Presentation: 福岡
• [Presentation] 歯周病原性細菌の新規オリゴペプチダーゼ：基質特異性と分子集合性 (2014)
  • Author(s): 根本孝幸，根本優子，小野俊雄，下山 佑，木村重信
  • Organizer: 第56回歯科基礎医学会学術大会・総会
  • Place of Presentation: 福岡
• [Presentation] 歯周病原性細菌の新規ペプチダーゼの基質特異性と分子集合解離機構 (2014)
  • Author(s): 根本孝幸，小野俊雄，下山 佑，木村重信，根本優子
  • Organizer: 第87回日本生化学会大会
  • Place of Presentation: 京都
• [Presentation] Colonization of Porphyromonas gingivalis in the plaques from mothers and their children. (2012)
  • Author(s): Kimura, M., Shimoyama, Y., Ohara-Nemoto, Y., Kimura, S. and Tanaka, M.
  • Organizer: The 11th Congress of the European Academy of Pediatric Dentistry
  • Place of Presentation: Strasbourg, France
• [Presentation] Classification and essential amino acid residues of the dipeptidyl peptidase (DPP) 7/11 family in the Cytophaga-Flavobacterium-Bacteroides group. (2012)
  • Author(s): Ohara-Nemoto, Y., Abdur-Rouf, S., Hoshino, T., Shimoyama, Y., Kimura, S., Ono, T. and Nemoto, T.
  • Organizer: 112th General Meeting of the American Society for Microbiology
  • Place of Presentation: San Francisco, California, USA
• [Presentation] A novel fibronectin binding protein of Streptococcus intermedius. (2012)
  • Author(s): Sasaki, M., Kodama, Y., Shimoyama, Y. and Kimura, S.
  • Organizer: 90th General Session & Exhibition of the IADR
  • Place of Presentation: Iguaçu Falls, Brazil
• [Presentation] The aciduricity of Streptococcus anginosus.
  • Author(s): Sasaki, M., Kodama, Y., Shimoyama, Y. and Kimura, S.
  • Organizer: 91st General Session & Exhibition of the IADR
  • Place of Presentation: Seattle, WA, USA
• [Presentation] Distribution of Porphyromonas gingivalis fimA genotypes in children’s plaques.
  • Author(s): Shimoyama, Y., ＊Ohara-Nemoto, Y., Kimura, M., Sasaki, M., Kodama, Y., Tanaka, M. and Kimura, S.
  • Organizer: 113th General Meeting of the American Society for Microbiology
  • Place of Presentation: Denver, CO, USA
• [Presentation] Dipeptide production in Porphyromonas gingivalis mediated by dipeptidyl peptidases and gingipains.
  • Author(s): Ohara-Nemoto, Y., Rouf, S.M.A., Shimoyama, Y., Kimura, S. and Nemoto, T.K.
  • Organizer: 113th General Meeting of the American Society for Microbiology
  • Place of Presentation: Denver, CO, USA
• [Presentation] The involvement of Streptococcus anginosus infection and AID expression in oral cancers.
  • Author(s): Iwasaki, K., Sasaki, M., Kodama, Y., Matsumoto, N., Kimura, S. and Sugiyama, Y.
  • Organizer: 21st International Conference of Oral and Maxillofacial Surgery
  • Place of Presentation: Barcelona, Spain
• [Book] 最新歯科衛生士教本 疾病の成り立ち及び回復過程の促進２ 微生物学 (全国歯科衛生士教育協議会監修) (2012)
  • Author(s): 木村重信，中川一路，川端重忠，大原直也，小松澤 均
  • Total Pages: 183
  • Publisher: 医歯薬出版, 東京