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Identification and characterization of internal basal lamina/tooth enamel adhesion

Research Project

Project/Area Number 24592779
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Morphological basic dentistry
Research InstitutionTokyo Dental College

Principal Investigator

SAWADA Takashi  東京歯科大学, 歯学部, 准教授 (60125010)

Co-Investigator(Kenkyū-buntansha) SHIBAYAMA Kazuko  東京歯科大学, 歯学部, 講師 (60408317)
Project Period (FY) 2012-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords歯肉 / 接合上皮 / エナメル質 / ラミニン / アメロチン / 免疫組織細胞化学 / 基底膜 / 内側基底板 / 免疫電顕 / ニホンザル / ミニブタ / ラミニン5 / in situ hybridization / 免疫組織化学 / in situ ハイブリダイゼーション
Outline of Final Research Achievements

The goal of this study was to investigate the mechanism underling adhesion between the internal basal lamina and the mineralized enamel surface. Immunogold labeling was performed to localize amelotin and laminin 5 at the tooth/junctional epithelium interface.
The results show that laminin 5 is actively synthesized in junctional epithelial cells and that the products are incorporated into the internal basal lamina to maintain firm epithelial adhesion to the tooth enamel throughout life. On the other hand, amelotin molecules were preferentially localized on the tiny strands associated with basal lamina, suggesting that the molecules facilitate cell/enamel adhesion by promoting mineralization of the strands.

Report

(5 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (6 results)

All 2015 2014 2013 2012

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Acknowledgement Compliant: 2 results) Presentation (2 results) Book (1 results)

  • [Journal Article] Ultrastructural and immunocytochemical characterization of ameloblast-enamel adhesion at maturation stage in amelogenesis in Macaca fuscata tooth germ2015

    • Author(s)
      Takashi Sawada
    • Journal Title

      Histochemistry and Cell Biology

      Volume: 144 Issue: 6 Pages: 587-596

    • DOI

      10.1007/s00418-015-1362-y

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Ultrastructural immunolocalization of laminin 332 (laminin 5) at dento-gingival interface in Macaca fuscata monkey2015

    • Author(s)
      Takashi Sawada, Takaki Yamazaki, Kazuko Shibayama, Yoko Yamaguchi, Mitsuhiro Ohshima
    • Journal Title

      MEDICAL MOLECULAR MORPHOLOGY

      Volume: - Issue: 2 Pages: 104-111

    • DOI

      10.1007/s00795-014-0085-9

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Expression and localization of laminin 5, laminin10, type IV collagen, and amelotin in adult murine gingiva2014

    • Author(s)
      Takashi Sawada, Takaki Yamazaki, Kazuko Shibayama, Kaido Kumazawa、Yoko Yamaguchi, Mitsuhiro Ohshima
    • Journal Title

      Journal of Molecular Histology

      Volume: 45 Issue: 3 Pages: 293-302

    • DOI

      10.1007/s10735-013-9559-7

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] ニホンザル歯-歯肉接合上皮におけるラミニン5の超微局在2013

    • Author(s)
      澤田隆
    • Organizer
      第54回日本組織細胞化学会総会・学術集会
    • Place of Presentation
      東京航空会館
    • Related Report
      2013 Research-status Report
  • [Presentation] Ultrastructural localization of laminin 5 at dentogingival border2013

    • Author(s)
      澤田隆
    • Organizer
      Microscopy Conference MC 2013
    • Place of Presentation
      Regensburg University, Germany
    • Related Report
      2012 Research-status Report
  • [Book] MICROSCOPY BOOK SERIES NUMBER 52012

    • Author(s)
      澤田隆
    • Publisher
      FORMATEX
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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