Identification and characterization of internal basal lamina/tooth enamel adhesion
| Project/Area Number |
24592779
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Tokyo Dental College |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
SHIBAYAMA Kazuko 東京歯科大学, 歯学部, 講師 (60408317)
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| Project Period (FY) |
2012-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 歯肉 / 接合上皮 / エナメル質 / ラミニン / アメロチン / 免疫組織細胞化学 / 基底膜 / 内側基底板 / 免疫電顕 / ニホンザル / ミニブタ / ラミニン５ / in situ hybridization / 免疫組織化学 / in situ ハイブリダイゼーション |
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| Outline of Final Research Achievements |
The goal of this study was to investigate the mechanism underling adhesion between the internal basal lamina and the mineralized enamel surface. Immunogold labeling was performed to localize amelotin and laminin 5 at the tooth/junctional epithelium interface.
The results show that laminin 5 is actively synthesized in junctional epithelial cells and that the products are incorporated into the internal basal lamina to maintain firm epithelial adhesion to the tooth enamel throughout life. On the other hand, amelotin molecules were preferentially localized on the tiny strands associated with basal lamina, suggesting that the molecules facilitate cell/enamel adhesion by promoting mineralization of the strands.
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Report
(5 results)
Research Products
(6 results)
