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Molecular regulation governing the bilateral palatal shelf adhesion and causing cleft palate in embryonic mice

Research Project

Project/Area Number 24592780
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Morphological basic dentistry
Research InstitutionThe Nippon Dental University

Principal Investigator

TAYA YUJI  日本歯科大学, 生命歯学部, 准教授 (30197587)

Co-Investigator(Kenkyū-buntansha) SOENO Yuuichi  日本歯科大学, 生命歯学部, 講師 (70350139)
SATO Kaori  日本歯科大学, 生命歯学部, 講師 (90287772)
AOBA Takaaki  日本歯科大学, 生命歯学部, 教授 (30028807)
FUJITA Kazuya  日本歯科大学, 生命歯学部, 助教 (70549055)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywords歯学 / 病理学 / マウス胎仔 / 二次口蓋発生 / 突起間接着 / 口蓋裂 / 遺伝子発現 / microRNA / 上皮間葉相互作用 / miRNA / 器官発生 / マウスモデル / 顎顔面発生 / 裂奇形 / 分子ネットワーク / 遺伝子ネットワーク
Outline of Final Research Achievements

We herein focused on the molecular mechanism governing the cell adhesion of the medial edge epithelia (MEE) of secondary palatal shelves in mouse embryos. To this end, we dissected out of MEE cells of palatal shelves at E14.0-14.5. The expression levels and subcellular localizations of Cask and its related genes/proteins in MEE cells were examined using DNA microarray analysis, LMD/real-time PCR and immunohistochemistry. F11r and Cdkn1a were detected only in MEE cells at the stage of contact, while Cask, Dlg1, Lin7c, Id1 and Tcfe2a were expressed in both MEE and mesenchymal cells. 7 miRNAs regulating the expression of F11r and Cdkn1a were also identified. The nuclear translocation of Cask in MEE cells at the same stage was verified. Our data suggest that Cask and its related molecules contribute to not only the cell adhesion but also cell cycle arrest of MEE at the stage of contact and unique miRNAs may act on their events through the regulation of Cask-related gene expression.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (19 results)

All 2014 2013 2012 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Open Access: 1 results) Presentation (7 results) Book (5 results) Remarks (3 results)

  • [Journal Article] Molecular contribution to cleft palate production in cleft lip mice2014

    • Author(s)
      Sasaki Y, Taya Y, Saito K, Fujita K, Aoba T, and Fujiwara T
    • Journal Title

      Congenital Anomalies

      Volume: 54 Issue: 2 Pages: 94-99

    • DOI

      10.1111/cga.12038

    • NAID

      120006986612

    • Related Report
      2014 Annual Research Report 2013 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Molecular signaling at the fusion stage of the mouse mandibular arch: involvement of insulin-like growth factor family.2013

    • Author(s)
      Kazuya Fujita
    • Journal Title

      Int. J. Dev. Biol.

      Volume: 57 Issue: 5 Pages: 1-8

    • DOI

      10.1387/ijdb.120110ys

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Generation of a mouse model with down-regulated U50 snoRNA (SNORD50) expression and its organ-specific phenotypic modulation2013

    • Author(s)
      Soeno Y, Fujita K, Kudo T, Asagiri M, Kakuta S, Taya Y, Shimazu Y, Sato K, Tanaka-Fujita R, Kubo S, Iwakura Y, Nakamura Y, Mori S, and Aoba T
    • Journal Title

      PLoS ONE

      Volume: 8 Issue: 8 Pages: e72105-e72105

    • DOI

      10.1371/journal.pone.0072105

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Progression of oral squamous cell carcinoma accompanied with reducedE-cadherin expression but not cadherin switch2012

    • Author(s)
      Hashimoto T., Soeno Y., Maeda H., Taya Y., Aoba T., Nasu M., Kawashiri S., Imai K
    • Journal Title

      PLOS ONE

      Volume: 7 Issue: 10 Pages: 1-8

    • DOI

      10.1371/journal.pone.0047899

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] マウス舌形態形成におけるリンパ管発生と分子制御2013

    • Author(s)
      田谷雄二、藤田和也、添野雄一、島津徳人、佐藤かおり、青葉孝昭
    • Organizer
      第55回歯科基礎医学会学術大会ならびに総会
    • Place of Presentation
      岡山県 岡山コンベンションセンター
    • Related Report
      2013 Research-status Report
  • [Presentation] 脈管構造の組織立体構築とVirtual Relity観察2013

    • Author(s)
      島津徳人,田谷雄二,添野雄一,白子要一,藤田和也,佐藤かおり,青葉孝昭
    • Organizer
      第55回歯科基礎医学会学術大会ならびに総会
    • Place of Presentation
      岡山県 岡山コンベンションセンター
    • Related Report
      2013 Research-status Report
  • [Presentation] Three-dimensional visualization of developing vascular-nervous networks in embryonic mice.

    • Author(s)
      Yuji Taya
    • Organizer
      2012 Sino-Japan Dental Conference
    • Place of Presentation
      四川大学(中国)
    • Related Report
      2012 Research-status Report
  • [Presentation] 腎内血管走行の3次元構築:弓状小葉間動脈の分枝と微小血管網.

    • Author(s)
      上杉憲子
    • Organizer
      第101回日本病理学会総会
    • Place of Presentation
      京王プラザホテル(東京都)
    • Related Report
      2012 Research-status Report
  • [Presentation] 癌微小環境におけるHIF-1α陽性核の空間分布と血管新生誘導.

    • Author(s)
      藤田和也
    • Organizer
      第101回日本病理学会総会
    • Place of Presentation
      京王プラザホテル(東京都)
    • Related Report
      2012 Research-status Report
  • [Presentation] Embryonic morphogenesis and organization of vascular and nervous networks in mouse craniofacial region.

    • Author(s)
      Yuji Taya
    • Organizer
      第64回日本細胞生物学会・第45回日本発生生物学会合同大会
    • Place of Presentation
      神戸国際会議場(兵庫県)
    • Related Report
      2012 Research-status Report
  • [Presentation] マウス舌発生における舌筋前駆細胞の移住は舌下神経の軸索誘導に働く.

    • Author(s)
      田谷雄二
    • Organizer
      第54回歯科基礎医学会学術大会ならびに総会
    • Place of Presentation
      奥羽大学(福島県)
    • Related Report
      2012 Research-status Report
  • [Book] ㈱キタ・メディア2014

    • Author(s)
      青葉孝昭監修 佐藤かおり、田谷雄二、添野雄一編集
    • Total Pages
      127
    • Publisher
      歯と歯周組織の病理学(冊子版)
    • Related Report
      2014 Annual Research Report
  • [Book] 歯学生のための最新・病態病理学入門(epub版)2014

    • Author(s)
      日本歯科大学 病理学講座編 田谷雄二分担執筆
    • Total Pages
      177
    • Publisher
      ㈱杏林社
    • Related Report
      2014 Annual Research Report
  • [Book] 歯と歯周組織の病理学(EPUB版)2014

    • Author(s)
      青葉孝昭監修 佐藤かおり、田谷雄二、添野雄一編集
    • Total Pages
      127
    • Publisher
      ㈱杏林社
    • Related Report
      2014 Annual Research Report
  • [Book] 歯学生のための最新・病態病理学入門2014

    • Author(s)
      青葉孝昭 監修、佐藤かおり 編集、田谷雄二・添野雄一 分担執筆
    • Total Pages
      177
    • Publisher
      株式会社キタ・メディア
    • Related Report
      2013 Research-status Report
  • [Book] 今すぐ始める組織立体構築―ImageJ/Fijiの活用プロトコール―2013

    • Author(s)
      島津徳人 編、青葉孝昭 監修、田谷雄二・添野雄一・佐藤かおり 執筆
    • Total Pages
      22
    • Publisher
      日本ビジュアルサイエンス株式会社
    • Related Report
      2013 Research-status Report
  • [Remarks] 日本歯科大学生命歯学部病理学講座HP

    • Related Report
      2014 Annual Research Report
  • [Remarks] 日本歯科大学病理学講座HP

    • URL

      http://www.ndu.ac.jp/~pathhome/

    • Related Report
      2013 Research-status Report
  • [Remarks] 日本歯科大学病理学講座HP

    • URL

      http://www.ndu.ac.jp/~pathhome/

    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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