The role of the initial colonizer Actinomyces oris in dental plaque formation.
Project/Area Number |
24592790
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Morphological basic dentistry
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Research Institution | Osaka Dental University |
Principal Investigator |
MASHIMO Chiho 大阪歯科大学, 歯学部, 講師 (80368159)
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Project Period (FY) |
2012-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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Keywords | 口腔細菌 / バイオフィルム / アクチノマイセス属細菌 / 初期付着菌 / アクチノマイセス / イニシャルコロナイザー / 口腔バイオフィルム / 遺伝子改変 / プラスミド / ミュータジェネシス / バイオフィルム形成関連遺伝子 |
Outline of Final Research Achievements |
Recent studies have revealed that oral Actinomyces spp. plays important roles for oral multispecies biofilm development as an initial colonizer. To identify gene(s) responsible for the extracellular polymeric substances (EPS) production, transposon mutagenesis was performed against a clinical isolate of A. oris strain K20. We reported the identification of genes that were predicted to be involved in the EPS biosynthesis and were essential for biofilm formation. We also developed the genetic engineering systems in Actinomyces spp. based on pJRD215 with high transformation efficiency.
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Report
(5 results)
Research Products
(16 results)
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[Journal Article] Identification of the genes involved in the biofilm-like structures on Actinomyces oris K20, a clinical isolate from an apical lesion2013
Author(s)
Mashimo C, Kamitani M, Nambu T, Yamane K, Yamanaka T, Tatami T, Inoue J, Kamei M, Morita S, Fukushima H
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Journal Title
Jounal of Endodontics
Volume: 39(1)
Issue: 1
Pages: 44-48
DOI
Related Report
Peer Reviewed
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