The role of the initial colonizer Actinomyces oris in dental plaque formation.

• Project/Area Number: 24592790
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Morphological basic dentistry
• Research Institution: Osaka Dental University
• Principal Investigator: MASHIMO Chiho 大阪歯科大学, 歯学部, 講師 (80368159)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 口腔細菌 / バイオフィルム / アクチノマイセス属細菌 / 初期付着菌 / アクチノマイセス / イニシャルコロナイザー / 口腔バイオフィルム / 遺伝子改変 / プラスミド / ミュータジェネシス / バイオフィルム形成関連遺伝子

Outline of Final Research Achievements

Recent studies have revealed that oral Actinomyces spp. plays important roles for oral multispecies biofilm development as an initial colonizer. To identify gene(s) responsible for the extracellular polymeric substances (EPS) production, transposon mutagenesis was performed against a clinical isolate of A. oris strain K20. We reported the identification of genes that were predicted to be involved in the EPS biosynthesis and were essential for biofilm formation. We also developed the genetic engineering systems in Actinomyces spp. based on pJRD215 with high transformation efficiency.

Research Products

• [Journal Article] Variations of nitrate-reducing activity in oral Rothia mucilaginosa. (2015)
  • Author(s): Mashimo C, Tsuzukibashi O, Uchibori S, Nambu T.
  • Journal Title: Journal of oral tissue engineering Volume: 13 Pages: 18-26
  • NAID: 130005150538
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Complete genome sequence of Rothia mucilaginosa strain NUM-Rm6536, isolated from a human oral cavity. (2015)
  • Author(s): Nambu T, Tsuzukibashi O, Uchibori S, Mashimo C.
  • Journal Title: Genome Announcements Volume: 3 Issue: 5
  • DOI: 10.1128/genomea.01122-15
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Complete Genome Sequence of Prevotella intermedia Strain 17-2 (2015)
  • Author(s): Nambu T, Yamane K, Maruyama H, Mashimo C, Yamanaka T.
  • Journal Title: Genome Announc Volume: 3 Issue: 4
  • DOI: 10.1128/genomea.00951-15
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Identification of disulphide stress-responsive extracytoplasmic function sigma factors in Rothia mucilaginosa (2013)
  • Author(s): Nambu T, Yamane K, Yamanaka T, Mashimo C, Maruyama H, Yoshida M, Hayashi H, Fukushima H
  • Journal Title: Archives of Oral Biology Volume: 58(6) Issue: 6 Pages: 681-689
  • DOI: 10.1016/j.archoralbio.2012.10.017
  • Peer Reviewed
• [Journal Article] Identification of the genes involved in the biofilm-like structures on Actinomyces oris K20, a clinical isolate from an apical lesion (2013)
  • Author(s): Mashimo C, Kamitani M, Nambu T, Yamane K, Yamanaka T, Tatami T, Inoue J, Kamei M, Morita S, Fukushima H
  • Journal Title: Jounal of Endodontics Volume: 39(1) Issue: 1 Pages: 44-48
  • DOI: 10.1016/j.joen.2012.08.009
  • Peer Reviewed
• [Journal Article] 広宿主域接合プラスミドpJRD215を基盤にした新規プラスミドの作製と口腔Actinomyces属細菌への応用. (2013)
  • Author(s): 真下千穂, 南部隆之, 円山由郷, 山根一芳, 山中武志, 福島久典.
  • Journal Title: Bacterial Adherence & Biofilm. Volume: 26 Pages: 61-64
• [Presentation] 口腔Rothia属細菌における硝酸還元活性の株間差異についての検討. (2016)
  • Author(s): 真下千穂, 円山由郷, 山根一芳, 山中武志, 王宝禮, 南部隆之.
  • Organizer: 第89回日本細菌学会総会
  • Place of Presentation: 大阪国際交流センター（大阪市天王寺区）
  • Year and Date: 2016-03-23
• [Presentation] 口腔Rothia属細菌における硝酸還元活性の株間差異についての検討. (2015)
  • Author(s): 真下千穂, 南部隆之.
  • Organizer: 第57回歯科基礎医学会学術大会・総会
  • Place of Presentation: 朱鷺メッセ新潟コンベンションセンター（新潟県、新潟市）
  • Year and Date: 2015-09-12
• [Presentation] 口腔Actinomyces属および口腔Rothia属細菌用遺伝子改変システムの開発. (2014)
  • Author(s): 真下千穂, 南部隆之, 円山由郷，山根一芳, 山中武志, 福島久典.
  • Organizer: 第56回歯科基礎医学会学術大会・総会
  • Place of Presentation: 福岡国際会議場（福岡県、福岡市）
  • Year and Date: 2014-09-27
• [Presentation] Development of genetic engineering systems in Actinomyces spp. (2014)
  • Author(s): Mashimo C, Nambu T, Maruyama Y, Yamane K, Yamanaka T, Fukushima H.
  • Organizer: FEBS EMBO Paris 2014
  • Place of Presentation: Palais des congres de Paris（フランス国、パリ市）
  • Year and Date: 2014-08-30 – 2014-09-04
• [Presentation] Actinomyces属細菌用クローニング・発現プラスミドの作製 (2014)
  • Author(s): 真下千穂、南部隆之、山根一芳、山中武志、福島久典
  • Organizer: 第87回細菌学会総会
  • Place of Presentation: 東京都江戸川区、タワーホール船堀
• [Presentation] Development of new plasmids for cloning and protein expression in Actinomyces spp (2013)
  • Author(s): C.Mashimo, T.Nambu, H.Maruyama, K.Yamane, T.Yamanaka, H.Fukushima
  • Organizer: The EMBO Meeting 2013
  • Place of Presentation: Amsterdam、The Netherlands
• [Presentation] pJRD215を基盤にしたクローニング・発現用プラスミドの作製とActinomyces属細菌への応用. (2013)
  • Author(s): 真下千穂, 南部隆之, 山根一芳, 山中武志, 福島久典.
  • Organizer: 第86回日本細菌学会総会
  • Place of Presentation: 千葉市, 幕張メッセ.
• [Presentation] Development of new plasmids for cloning and expression in oral Actinomyces spp. (2012)
  • Author(s): Mashimo C, Nambu T, Maruyama H, Yamane K, Yamanaka T, Fukushima H.
  • Organizer: 4th EMBO meeting 2012
  • Place of Presentation: Nice, France.
• [Presentation] 広宿主域接合プラスミドpJRD215を基盤にした新規プラスミドの作製と口腔Actinomyces属細菌への応用.
  • Author(s): 真下千穂, 南部隆之, 円山由郷, 山根一芳, 山中武志, 福島久典.
  • Organizer: 第26回Bacterial Adherence & Biofilm学術集会
  • Place of Presentation: 大阪市, 大阪ガーデンパレス
• [Presentation] Actinomyces臨床分離株におけるバイオフィルム形成関連遺伝子の網羅的解析.
  • Author(s): 真下千穂, 南部隆之, 山根一芳, 山中武志, 森田章介, 福島久典.
  • Organizer: 第535回大阪歯科学会例会
  • Place of Presentation: 大阪府枚方市, 大阪歯科大学.