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The role of the initial colonizer Actinomyces oris in dental plaque formation.

Research Project

Project/Area Number 24592790
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Morphological basic dentistry
Research InstitutionOsaka Dental University

Principal Investigator

MASHIMO Chiho  大阪歯科大学, 歯学部, 講師 (80368159)

Project Period (FY) 2012-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords口腔細菌 / バイオフィルム / アクチノマイセス属細菌 / 初期付着菌 / アクチノマイセス / イニシャルコロナイザー / 口腔バイオフィルム / 遺伝子改変 / プラスミド / ミュータジェネシス / バイオフィルム形成関連遺伝子
Outline of Final Research Achievements

Recent studies have revealed that oral Actinomyces spp. plays important roles for oral multispecies biofilm development as an initial colonizer. To identify gene(s) responsible for the extracellular polymeric substances (EPS) production, transposon mutagenesis was performed against a clinical isolate of A. oris strain K20. We reported the identification of genes that were predicted to be involved in the EPS biosynthesis and were essential for biofilm formation. We also developed the genetic engineering systems in Actinomyces spp. based on pJRD215 with high transformation efficiency.

Report

(5 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (16 results)

All 2016 2015 2014 2013 2012 Other

All Journal Article (6 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 5 results,  Open Access: 3 results,  Acknowledgement Compliant: 3 results) Presentation (10 results)

  • [Journal Article] Variations of nitrate-reducing activity in oral Rothia mucilaginosa.2015

    • Author(s)
      Mashimo C, Tsuzukibashi O, Uchibori S, Nambu T.
    • Journal Title

      Journal of oral tissue engineering

      Volume: 13 Pages: 18-26

    • NAID

      130005150538

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Complete genome sequence of Rothia mucilaginosa strain NUM-Rm6536, isolated from a human oral cavity.2015

    • Author(s)
      Nambu T, Tsuzukibashi O, Uchibori S, Mashimo C.
    • Journal Title

      Genome Announcements

      Volume: 3 Issue: 5

    • DOI

      10.1128/genomea.01122-15

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Complete Genome Sequence of Prevotella intermedia Strain 17-22015

    • Author(s)
      Nambu T, Yamane K, Maruyama H, Mashimo C, Yamanaka T.
    • Journal Title

      Genome Announc

      Volume: 3 Issue: 4

    • DOI

      10.1128/genomea.00951-15

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Identification of disulphide stress-responsive extracytoplasmic function sigma factors in Rothia mucilaginosa2013

    • Author(s)
      Nambu T, Yamane K, Yamanaka T, Mashimo C, Maruyama H, Yoshida M, Hayashi H, Fukushima H
    • Journal Title

      Archives of Oral Biology

      Volume: 58(6) Issue: 6 Pages: 681-689

    • DOI

      10.1016/j.archoralbio.2012.10.017

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Identification of the genes involved in the biofilm-like structures on Actinomyces oris K20, a clinical isolate from an apical lesion2013

    • Author(s)
      Mashimo C, Kamitani M, Nambu T, Yamane K, Yamanaka T, Tatami T, Inoue J, Kamei M, Morita S, Fukushima H
    • Journal Title

      Jounal of Endodontics

      Volume: 39(1) Issue: 1 Pages: 44-48

    • DOI

      10.1016/j.joen.2012.08.009

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] 広宿主域接合プラスミドpJRD215を基盤にした新規プラスミドの作製と口腔Actinomyces属細菌への応用.2013

    • Author(s)
      真下千穂, 南部隆之, 円山由郷, 山根一芳, 山中武志, 福島久典.
    • Journal Title

      Bacterial Adherence & Biofilm.

      Volume: 26 Pages: 61-64

    • Related Report
      2012 Research-status Report
  • [Presentation] 口腔Rothia属細菌における硝酸還元活性の株間差異についての検討.2016

    • Author(s)
      真下千穂, 円山由郷, 山根一芳, 山中武志, 王宝禮, 南部隆之.
    • Organizer
      第89回日本細菌学会総会
    • Place of Presentation
      大阪国際交流センター(大阪市天王寺区)
    • Year and Date
      2016-03-23
    • Related Report
      2015 Annual Research Report
  • [Presentation] 口腔Rothia属細菌における硝酸還元活性の株間差異についての検討.2015

    • Author(s)
      真下千穂, 南部隆之.
    • Organizer
      第57回歯科基礎医学会学術大会・総会
    • Place of Presentation
      朱鷺メッセ新潟コンベンションセンター(新潟県、新潟市)
    • Year and Date
      2015-09-12
    • Related Report
      2015 Annual Research Report
  • [Presentation] 口腔Actinomyces属および口腔Rothia属細菌用遺伝子改変システムの開発.2014

    • Author(s)
      真下千穂, 南部隆之, 円山由郷,山根一芳, 山中武志, 福島久典.
    • Organizer
      第56回歯科基礎医学会学術大会・総会
    • Place of Presentation
      福岡国際会議場(福岡県、福岡市)
    • Year and Date
      2014-09-27
    • Related Report
      2014 Research-status Report
  • [Presentation] Development of genetic engineering systems in Actinomyces spp.2014

    • Author(s)
      Mashimo C, Nambu T, Maruyama Y, Yamane K, Yamanaka T, Fukushima H.
    • Organizer
      FEBS EMBO Paris 2014
    • Place of Presentation
      Palais des congres de Paris(フランス国、パリ市)
    • Year and Date
      2014-08-30 – 2014-09-04
    • Related Report
      2014 Research-status Report
  • [Presentation] Actinomyces属細菌用クローニング・発現プラスミドの作製2014

    • Author(s)
      真下千穂、南部隆之、山根一芳、山中武志、福島久典
    • Organizer
      第87回細菌学会総会
    • Place of Presentation
      東京都江戸川区、タワーホール船堀
    • Related Report
      2013 Research-status Report
  • [Presentation] Development of new plasmids for cloning and protein expression in Actinomyces spp2013

    • Author(s)
      C.Mashimo, T.Nambu, H.Maruyama, K.Yamane, T.Yamanaka, H.Fukushima
    • Organizer
      The EMBO Meeting 2013
    • Place of Presentation
      Amsterdam、The Netherlands
    • Related Report
      2013 Research-status Report
  • [Presentation] pJRD215を基盤にしたクローニング・発現用プラスミドの作製とActinomyces属細菌への応用.2013

    • Author(s)
      真下千穂, 南部隆之, 山根一芳, 山中武志, 福島久典.
    • Organizer
      第86回日本細菌学会総会
    • Place of Presentation
      千葉市, 幕張メッセ.
    • Related Report
      2012 Research-status Report
  • [Presentation] Development of new plasmids for cloning and expression in oral Actinomyces spp.2012

    • Author(s)
      Mashimo C, Nambu T, Maruyama H, Yamane K, Yamanaka T, Fukushima H.
    • Organizer
      4th EMBO meeting 2012
    • Place of Presentation
      Nice, France.
    • Related Report
      2012 Research-status Report
  • [Presentation] 広宿主域接合プラスミドpJRD215を基盤にした新規プラスミドの作製と口腔Actinomyces属細菌への応用.

    • Author(s)
      真下千穂, 南部隆之, 円山由郷, 山根一芳, 山中武志, 福島久典.
    • Organizer
      第26回Bacterial Adherence & Biofilm学術集会
    • Place of Presentation
      大阪市, 大阪ガーデンパレス
    • Related Report
      2012 Research-status Report
  • [Presentation] Actinomyces臨床分離株におけるバイオフィルム形成関連遺伝子の網羅的解析.

    • Author(s)
      真下千穂, 南部隆之, 山根一芳, 山中武志, 森田章介, 福島久典.
    • Organizer
      第535回大阪歯科学会例会
    • Place of Presentation
      大阪府枚方市, 大阪歯科大学.
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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