Role of Monad-interacting complex on tumorigenesis

• Project/Area Number: 24592795
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Functional basic dentistry
• Research Institution: Niigata University (2013-2014); Osaka University (2012)
• Principal Investigator: SAEKI Makio 新潟大学, 医歯学系, 教授 (30273692)
• Co-Investigator(Kenkyū-buntansha): 上崎 善規 大阪大学, 歯学研究科(研究院), 名誉教授 (40116017)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: R2TP / Monad / mTOR / Tumorigenesis / PIH1D1 / ｍＴＯＲ / RPAP3 / breast cancer / invasion

Outline of Final Research Achievements

R2TP was originally identified in yeast Saccharomyces cerevisiae as Hsp90 interacting complex, and is composed of four different proteins: Rvb1, Rvb2, Tah1, and Pih1. This complex is well-conserved in eukaryotes, and is involved in many cellular processes such as snoRNP biogenesis, RNA polymerase assembly, PIKK signaling, and apoptosis. An increasing number of research related
to R2TP suggests a linkage of its function with tumorigenesis. We provide an overview of several recent studies on R2TP that are related to cell proliferation and carcinogenesis, and propose a possible role of R2TP in tumorigenesis through regulating snoRNA/snoRNP biogenesis.

Research Products

• [Journal Article] Comparative Analysis of Mouse Induced Pluripotent Stem Cells and Mesenchymal Stem Cells during Osteogenic Differentiation in vitro. (2014)
  • Author(s): Egusa, H., Kayashima, H., Miura, J., Uraguchi, S., Wang, F., Okawa, H., Sasaki, J.I., Saeki, M., Matsumoto, T., and Yatani, H.
  • Journal Title: Stem cells and development. Volume: 23 Issue: 18 Pages: 2156-2169
  • DOI: 10.1089/scd.2013.0344
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] R2TP chaperone complex: its involvement in snoRNP assembly and tumorigenesis. (2014)
  • Author(s): Kakihara, Y., Saeki, M.
  • Journal Title: R2TP chaperone complex: its involvement in snoRNP assembly and tumorigenesis. Volume: 15 Issue: 6 Pages: 404-404
  • DOI: 10.1515/bmc-2014-0028
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Exosome-bound WD repeat protein Monad inhibits breast cancer cell invasion by degrading amphiregulin mRNA. (2013)
  • Author(s): Saeki, M., Egusa, H., Kamano, Y., Kakihara, Y., Houry, W.A., Yatani, H., Noguchi, S., and Kamisaki, Y
  • Journal Title: PloS one Volume: 8, Issue: 7 Pages: e67326-e67326
  • DOI: 10.1371/journal.pone.0067326
  • Peer Reviewed
• [Journal Article] PIH1D1 interacts with mTOR complex 1 and enhances ribosome RNA transcription (2013)
  • Author(s): Kamano, Y., Saeki, M., Egusa, H., Kakihara, Y., Houry, W.A., Yatani, H., and Kamisaki, Y
  • Journal Title: FEBS letters Volume: 587 Issue: 20 Pages: 3303-3308
  • DOI: 10.1016/j.febslet.2013.09.001
  • Peer Reviewed
• [Journal Article] RPAP3 splicing variant isoform 1 interacts with PIH1D1 to compose R2TP complex for cell survival. (2013)
  • Author(s): Yoshida M, Saeki M, Egusa H, Irie Y, Kamano Y, Uraguchi S, Sotozono M, Niwa H, Kamisaki Y.
  • Journal Title: Biochem Biophys Res Commun. Volume: 430 Issue: 1 Pages: 320-4
  • DOI: 10.1016/j.bbrc.2012.11.017
  • Peer Reviewed
• [Presentation] Monad(WDR92)とR2TP複合体の細胞死における役割 (2012)
  • Author(s): 佐伯万騎男
  • Organizer: 第21回日本Cell Death学会
  • Place of Presentation: 名古屋
  • Invited
• [Presentation] R2TP complex regulates apoptosis (2012)
  • Author(s): Saeki M
  • Organizer: First International Meeting on Reptin and Pontin
  • Place of Presentation: ボルドー、フランス
  • Invited