Screening for regulatory proteins of osteoclastogenesis with lentiviral shRNA library
Project/Area Number |
24592806
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Functional basic dentistry
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Research Institution | Saga University |
Principal Investigator |
Kukita Akiko 佐賀大学, 医学部, 准教授 (30153266)
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Co-Investigator(Kenkyū-buntansha) |
Kukita Toshio 九州大学, 大学院歯学研究院, 教授 (70150464)
|
Co-Investigator(Renkei-kenkyūsha) |
Nishioka Kenichi 佐賀大学, 医学部, 講師 (80370120)
Kuhara Satoru 九州大学, 大学院農学研究院, 教授 (00153320)
|
Project Period (FY) |
2012-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
Keywords | 破骨細胞分化 / shRNAライブラリー / スクリーニング / 破骨細胞 / shRNA / 分化 / 細胞分化 / 膜タンパク質 / RANKL / マクロファージ / レポーター細胞 |
Outline of Final Research Achievements |
We performed the screening for proteins which have essential roles in osteoclast differentiation and function by using shRNA lentivirus library that target signaling pathway in mouse. First, we prepared a reporter cell line RAW-ctsk-Venus1 which induce the expression of fluorescent protein Venus under the control of cathepsin K promoter. RANKL dose dependently induced Venus expression in RAW-ctsk-Venus1. Surface expression of population of Venus-positive cells was analyzed. RANK expression of Venus low-positive cells was lower than that of Venus high-positive cells. RAW-ctsk-Venus 1 cells were infected by shRNA lentivirus library and stimulated with RANKL. The fraction of Venus low-positive cells affected by infection were collected by cell sorter. Analyzing of enriched population of shRNA in Venus low-positive cells revealed that several candidates for regulators of osteoclastogenesis including some signaling molecules Map3k and Vav3.
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Report
(5 results)
Research Products
(39 results)
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[Journal Article] Interferon-gamma improves impaired dentinogenic and immunosuppressive functions of irreversible pulpitis-derived human dental pulp stem cells.2016
Author(s)
Sonoda S, Yamaza H, Ma L, Tanaka Y, Tomoda E, Aijima R, Nonaka K, Kukita T, Shi S, Nishimura F, Yamaza T.
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Journal Title
Sci Rep.
Volume: 6
Issue: 1
Pages: 19286-19286
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Generation mechanism of RANKL+ effector memory B cells: its relevance to the pathogenesis of rheumatoid arthritis.2016
Author(s)
Ota Y., Niiro H., Ota S., Ueki N., Tsuzuki H., Nakayama T., Mishima K., Higashioka K., Jabbarzadeh-Tabrizi S., Mitoma H., Akahoshi M., Arinobu Y., Kukita A., Yamada H., Tsukamoto H., Akashi K.
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Journal Title
Arthritis Reseach & Therapy
Volume: -
Related Report
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[Journal Article] Mesenchymal stem cells markedly suppress inflammatory bone destruction in rats with adjuvant-induced arthritis2014
Author(s)
Takano, T. Li, Y. J. Kukita, A. Yamaza, T. Ayukawa, Y. Moriyama, K. Uehara, N. Nomiyama, H. Koyano, K. and Kukita, T.
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Journal Title
Laboratory Investigation
Volume: 94
Issue: 3
Pages: 286-296
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Involvement of CXCL14 in osteolytic bone metastasis from lung cancer.2014
Author(s)
Takiguchi S, Korenaga N, Inoue K, Sugi E, Kataoka Y, Matsusue K, Futagami K, Li YJ, Kukita T, Teramoto N, Iguchi H.
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Journal Title
Int J Oncol.
Volume: 44(4)
Issue: 4
Pages: 1316-1324
DOI
Related Report
Peer Reviewed / Open Access
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[Book] Adenosine receptor2015
Author(s)
P-F. Qu, A. Kukita, Y-J. Li, K. Moriyama, L. Lei, T. Kukita
Total Pages
21
Publisher
Nova Science Publishers, Inc. New York
Related Report