The role of lysine-specific gingipain in inflammatory bone loss in periodontitis.
Project/Area Number |
24592817
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Functional basic dentistry
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Research Institution | Showa University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
TAKAMI Masamichi 昭和大学, 歯学部, 教授 (80307058)
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Co-Investigator(Renkei-kenkyūsha) |
IMAMURA Takahisa 熊本大学, 大学院生命科学研究部, 准教授 (20176499)
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Research Collaborator |
AKIYAMA Tomohito 昭和大学, 歯学部, 助教 (90710319)
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Project Period (FY) |
2012-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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Keywords | 歯周病 / 破骨細胞 / プロテアーゼ / 骨代謝 / ジンジパイン / 骨 / タンパク質分解酵素 |
Outline of Final Research Achievements |
Periodontitis is a chronic inflammatory disease accompanied by alveolar bone loss. Porphyromonas gingivalis, which plays a key role in the etiology of periodontitis, produces cysteine proteases called gingipains. Gingipains are classified into two groups based on their cleavage site specificity, i.e., Rgp and Kgp. We found that osteoclast differentiation induced by inflammatory cytokines such as TNF-α and IL-1β in co-cultures of mouse osteoblasts and bone marrow cells, whereas RgpB had no effect on osteoclast differentiation under the same experimental conditions. Osteoprotegerin (OPG), a protein that negatively regulates osteoclast differentiation, was degraded by Kgp. Kgp primarily cleaved death-domain-like region of OPG; the fragments containing RANKL-biding domain lost its binding capacity to RANKL. Our data suggests that degradation of OPG by Kgp is a crucial event in the progression of osteolysis in periodontitis.
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Report
(4 results)
Research Products
(39 results)
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[Journal Article] Porphyromonas gingivalis-derived lysine gingipain enhances osteoclast differentiation induced by tumor necrosis factor and interleukin-1 but suppresses that by interleukin-17: Importance of proteolytic degradation of osteoprotegerin by lysine gingipain.2014
Author(s)
Tomohito Akiyama, Yoichi Miyamoto, Kentaro Yoshimura, Atsushi Yamada, Masamichi Takami, Tetsuo Suzawa, Marie Hoshino, Takahisa Imamura, Chie Akiyama, Rika Yasuhara, Kenji Mishima, Toshifumi Maruyama, Chikara Kohda, Kazuo Tanaka, Jan Potempa, Hisataka Yasuda, Kazuyoshi Baba, Ryutaro Kamijo.
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Journal Title
J.Biol.Chem.
Volume: 289
Issue: 22
Pages: 1-35
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Effects of antibody to receptor activator of nuclear factor κ-B ligand on inflammation and cartilage degradation in collagen antibody-induced arthritis in mice.2014
Author(s)
Funato S, Matsunaga A, Oh K, Miyamoto Y, Yoshimura K, Tanaka J, Suzuki D, Uyama R, Suzuki H, Mishima K, Nakamura M, Namiki O, Baba K, Inagaki K, Kamijo R.
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Journal Title
J Negat Results Biomed.
Volume: 13
Issue: 1
Pages: 18-18
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Octacalcium phosphate suppresses chondrogenic differentiation of ATDC5 cells.2013
Author(s)
I Shibuya, K Yoshimura, Y Miyamoto, A Yamada, M Takami, T Suzawa, D Suzuki, N Ikumi, F Hiura, T Anada, O Suzuki, R Kamijo.
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Journal Title
Cell Tissue Res
Volume: 352
Issue: 2
Pages: 401-412
DOI
Related Report
Peer Reviewed
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[Journal Article] Downregulation of carbonic anhydrase IX promotes Col10a1 expression in chondrocytes2013
Author(s)
Maruyama T, Miyamoto Y, Yamamoto G, Yamada A, Yoshimura K, Suzawa T, Takami M, Akiyama T, Hoshino M, Iwasa F, Ikumi N, Tachikawa T, Mishima K, Baba K, Kamijo R
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Journal Title
PLoS ONE
Volume: Vol.8
Issue: 2
Pages: e56984-e56984
DOI
Related Report
Peer Reviewed
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[Journal Article] BMP2 differentially regulates the expression of Gremlin1 and Gremlin2, the negative regulators of BMP function, during osteoblast differentiation2012
Author(s)
Suzuki D, Yamada A, Aizawa R, Funato S, Matsumoto T, Suzuki W, Takami M, Miyamoto Y, Suzawa T, Yamamoto M, Baba K, Kamijo R
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Journal Title
Calcif Tissue Int
Volume: 91
Issue: 1
Pages: 88-96
DOI
Related Report
Peer Reviewed
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[Journal Article] R848, a toll-like receptor 7 agonist, inhibits osteoclast differentiation but not survival or bone-resorbing function of mature osteoclasts2012
Author(s)
Miyamoto A, Takami M, Matsumoto A, Mochizuki A, Yamada T, Tachi K, Shibuya I, Nakamach, T, Shioda S, Baba K, Inoue T, Miyamoto Y, Yim M, Kamijo R
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Journal Title
Cytotechnology
Volume: 64
Issue: 3
Pages: 331-339
DOI
Related Report
Peer Reviewed
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[Presentation] 8-Nitro-guanosine 3’,5’-cyclic monophosphate mediates elongation of the growth plate cartilage in mice.2014
Author(s)
Hoshino M, Miyamoto Y, Kentaro Yoshimura K, Suzuki D, Akaike T, Mishima K, Baba K, Kamijo R
Organizer
The 2014 ASCB/IFCB Meeting
Place of Presentation
Philadelphia, PA, USA
Year and Date
2014-12-06 – 2014-12-11
Related Report
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[Presentation] Importance of proteolytic degradation of osteoprotegerin by lysine-specific gingipain in periodontal osteoclastogenesis.2012
Author(s)
Akiyama T, Miyamoto Y, Yamada A, Takami M, Yasuhara R, Yoshimura K, Hoshino M, Maruyama T, Mishima K, Baba K, Kamijo R
Organizer
ASBMR 2012 Annual Meeting
Place of Presentation
Minneapolis, MN, USA
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