Visualization of Neural circuits controlling feeding behavior

• Project/Area Number: 24592818
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Functional basic dentistry
• Research Institution: Nihon University
• Principal Investigator: KONDO Masahiro 日本大学, 歯学部, 講師 (50312294)
• Co-Investigator(Renkei-kenkyūsha): TAMURA Takuya 東京医科歯科大学, 難治疾患研究所, 助教 (80396647)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 神経回路 / 化学感覚 / 感覚

Outline of Final Research Achievements

To visualize central neurons responsible for taste perception, we made transgenic animals expressing thermo-sensitive TRP ion channel (dTRPA) in taste cells. Heat stimulation of the sweet- and bitter-cells induced different patterns of ERK phosphorylation in the subesophageal ganglion. In addition, we tried to identify the neurons controlling feeding behavior using enhancer trap lines expressing sodium channel in a subset of neurons in the brain. We found that excitability of subset of dopaminergic neurons modulates the feeding behavior.

Research Products

• [Journal Article] Morphological and functional changes in regenerated primary afferent fibres following mental and inferior alveolar nerve transection (2014)
  • Author(s): Tsuboi Y, Honda K, Bae YC, Shinoda M, Kondo M, Katagiri A, Echizenya S, Kamakura S, Lee J, Iwata K.
  • Journal Title: Eur J Pain Volume: - Issue: 9 Pages: 1258-1266
  • DOI: 10.1002/ejp.650
  • Peer Reviewed / Open Access
• [Journal Article] ERK is involved in tooth-pressure-induced Fos expression in Vc neurons (2012)
  • Author(s): Hasegawa M, Kondo M* et al.
  • Journal Title: J Dent Res Volume: 91(12) Issue: 12 Pages: 1141-1146
  • DOI: 10.1177/0022034512462397
  • Peer Reviewed / Open Access
• [Journal Article] Involvement of AMPA Receptor GluR2 and GluR3 Trafficking in Trigeminal Spinal Subnucleus Caudalis and C1/C2 Neurons in Acute-Facial Inflammatory Pain. (2012)
  • Author(s): Miyamoto, M.
  • Journal Title: PLoS One Volume: 7 Issue: 8 Pages: e44055-e44055
  • DOI: 10.1371/journal.pone.0044055
  • Peer Reviewed / Open Access
• [Presentation] 細胞接着分子Dscamの過剰発現により生じる感覚ニューロンの軸索投射異常 (2013)
  • Author(s): 近藤真啓
  • Organizer: 第6回分子高次機能研究会
  • Place of Presentation: 軽井沢ホテル（長野県）
• [Presentation] Extracellular signal-regulated kinase phosphorylation in trigeminal spinal nucleus and upper cervical spinal cord neurons induced by experimental tooth movement
  • Author(s): Shibuta K
  • Organizer: 第９０回日本生理学会大会
  • Place of Presentation: タワーホール船堀（東京）
• [Presentation] Overexpression of Dscam leads to axonal targeting defects in the Drosophila sensory neurons
  • Author(s): Kondo M
  • Organizer: 第３５回日本分子生物学会年会
  • Place of Presentation: マリンメッセ福岡（福岡）
• [Presentation] Neuronal Ig receptors control neural wiring specificity and feeding behavior in Drosophila
  • Author(s): Kondo M
  • Organizer: 10th International Symposium on Molecular and Neural Mechanisms of Taste and Olfactory Perception (YR Umami Forum 2012)
  • Place of Presentation: 九州大学馬出キャンパスコラボレーションセンター（福岡）