Decay of RNA binding protein involved in oral oncogenesis

• Project/Area Number: 24592824
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pathobiological dentistry/Dental radiology
• Research Institution: Hokkaido University
• Principal Investigator: ISHIKAWA MAKOTO 北海道大学, 大学病院, 准教授 (10202970)
• Co-Investigator(Kenkyū-buntansha): HIGASHINO Fumihiro 北海道大学, 大学院歯学研究科, 准教授 (50301891) ; SHINNDOU Masanobu 北海道大学, 大学院歯学研究科, 教授 (20162802) ; KITAMURA Tetsuya 北海道大学, 大学院歯学研究科, 助教 (00451451)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 口腔外科一般 / ARE-mRNA / pp32ｒｌ / HuR / caspase3 / 口腔がん / pp32r1 / pp32 / 核害輸送 / 安定化 / 核外輸送

Outline of Final Research Achievements

In this study, we examined how pp32r1 controls the decay of HuR to contribute tumorigenesis. In the cancer cells expressing high level pp32r1, the expression of HuR was up-regulated. pp32r1 inhibited the activity of caspase3, which is required for the HuR degradation. pp32r1 bound to HuR stronger than that of pp32. These findings indicate that pp32r1 plays the important role for transforming cells by inhibit caspase activity.

Research Products

• [Journal Article] HuR and podoplanin expression is correlated with high risk of malignant transformation in patients with orla preneoplastic lesions. (2015)
  • Author(s): Habiba U., Hida K., Higashino F., Kitamura T., Yanagawa-Matsuda A., Totsuka Y. and Shindoh M
  • Journal Title: Oncol Let, Volume: 印刷中
  • Peer Reviewed
• [Journal Article] pp32r1 controls the decay of the RNA-binding protein HuR. (2014)
  • Author(s): Imamachi K., Higashino F., Kitamura T., Kakuguchi W., Yanagawa-Matsuda A., Ishikawa M., Kitagawa Y., Totsuka Y. and Shindoh M.
  • Journal Title: Oncology Reports Volume: 31 Pages: 1103-1108
  • NAID: 120005465481
  • Peer Reviewed
• [Journal Article] Cytoplasmic expression of HuR may be a valuable diagnostic tool for determining the potential for malignant transformation of oral verrucous borderline lesions. (2014)
  • Author(s): Habiba U., Kitamura T., Yanagawa-Matsuda A., Hida K., Higashino F., Ohiro Y., Totsuka Y. and Shindoh M.
  • Journal Title: Oncology Reports Volume: in press
  • Peer Reviewed
• [Journal Article] E1A expression might be controlled by miR-214 in cells with low adenovirus productivity. (2012)
  • Author(s): Yanagawa-Matsuda A., Kitamura T., Higashino F., Yamano S., Totsuka Y. and Shindoh M
  • Journal Title: Virus Res. Volume: 170 Pages: 85-90
  • Peer Reviewed
• [Journal Article] Expression of parathyroid hormone-related protein confers malignant potential to mucoepidermoid carcinoma. (2012)
  • Author(s): Nagamine K., Kitamura T., Yanagawa-Matsuda A., Ohiro Y., Tei K., Hida K., Higashino F., Totsuka Y. and Shindoh M
  • Journal Title: Oncology Reports Volume: 29 Pages: 2114-2118
  • Peer Reviewed
• [Presentation] Expression patterns of cancer stem cell markers ALDH1 and podoplanin in oral leukoplakia and the risk of malignant transformation (2014)
  • Author(s): Habiba U, Kitamura T, Yanagawa-Matsuda A, Hida K, Higashino F, Totsuka Y, Shindoh M
  • Organizer: 第25回日本臨床口腔病理学会総会・学術大会
  • Place of Presentation: メディアシップ日報ホール（新潟）
  • Year and Date: 2014-08-27 – 2014-08-29
• [Presentation] Overexpression of HuR and Podoplanin predicts the development of oral cancer in patients with preneoplastic lesion. (2014)
  • Author(s): Habiba U., Kitamuta T., Yanagawa-Matsuda A., Hida K., Higashino F., Totsuka Y., Shindoh M
  • Organizer: 17th International Congress on Oral Pathology and Medicine,
  • Place of Presentation: Istanbul Military Museum (Istanbul, Turkey)
  • Year and Date: 2014-05-25 – 2014-05-30
• [Presentation] 発がん関連RNAタンパクHuRの分解抑制機構 (2014)
  • Author(s): 東野史裕、今待賢治、北村哲也、柳川-松田 彩、進藤正信
  • Organizer: 第68回日本口腔科学会学術集会
  • Place of Presentation: 京王プラザホテル（東京）
  • Year and Date: 2014-05-08 – 2014-05-09
• [Presentation] Podoplanin and HuR expression predict the malignancies in patients with oral epithelial dysplasia (2014)
  • Author(s): Umma Habiba, 北村哲也、柳川-松田 彩、樋田京子、東野史裕、進藤正信
  • Organizer: 第103回日本病理学会
  • Place of Presentation: 広島国際会議場（広島）
  • Year and Date: 2014-04-24 – 2014-04-26
• [Presentation] pp32r1はHuRと結合しクリベージを抑制することで細胞がん化誘導にはたらく (2013)
  • Author(s): 今待賢治、東野史裕、北村哲也、北川善政、進藤正信
  • Organizer: 第67回日本口腔科学会学術大会
  • Place of Presentation: 栃木県総合文化センター（宇都宮）
• [Presentation] 発がんに関わるRNA結合タンパクHuRの分解制御 (2013)
  • Author(s): 東野史裕、今待賢治、北村哲也、柳川-松田 彩、進藤正信
  • Organizer: 第24回日本臨床口腔病理学会総会・学術大会
  • Place of Presentation: 日本大学理工学部1号館 CSTホール(東京）
• [Presentation] Overexpression of podoplanin and HuR may predict the development of oral cancer in patients with oral epithelial dysplasia. (2013)
  • Author(s): Habiba U, Kitamura T, Yanagawa-Matsuda A, Hida K, Higashino F, Shindoh M
  • Organizer: 第24回日本臨床口腔病理学会総会・学術大会
  • Place of Presentation: 日本大学理工学部1号館 CSTホール(東京）
• [Presentation] 発がんを促進するRNA結合タンパクHuRの分解制御
  • Author(s): 東野史裕、今待賢治、北村哲也、松田 彩、進藤正信
  • Organizer: 第93回北海道医学大会病理分科会、第46回北海道病理談話会
  • Place of Presentation: 北海道大学医学部フラテ会館(札幌）
• [Presentation] Cytoplasmic expression of HuR could be a useful diagnostic tool to determine malignant transformation of oral verrucous borderline lesions.
  • Author(s): Habiba U, Kitamura T, Yanagawa-Matsuda A, Higashino F, Shindoh M
  • Organizer: 第23回日本臨床口腔病理学会総会・学術大会
  • Place of Presentation: 東京医科歯科大学（東京）
• [Presentation] 発がん活性をもつpp32r1はRNA結合タンパクHuRの分解を抑制する
  • Author(s): 今待賢治、東野史裕、北村哲也、柳川—松田 彩、進藤正信
  • Organizer: 第23回日本臨床口腔病理学会総会・学術大会
  • Place of Presentation: 東京医科歯科大学（東京）
• [Presentation] pp32r1はRNA結合タンパクHuRと結合しその分解を抑制する
  • Author(s): 今待賢治、北村哲也、柳川 彩、進藤正信、東野史裕
  • Organizer: 第71回日本癌学会総会
  • Place of Presentation: ロイトン札幌（札幌市）
• [Presentation] 発がん活性をもつpp32r1はHuRと結合しその分解を抑制する
  • Author(s): 今待賢治、北村哲也、柳川—松田 彩、進藤正信、東野史裕
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場（福岡市）