Visualization of immune-inhibitory signaling in memory formation

• Project/Area Number: 24592828
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pathobiological dentistry/Dental radiology
• Research Institution: University of Occupational and Environmental Health, Japan (2013-2014); Tokyo Medical and Dental University (2012)
• Principal Investigator: IWAI Yoshiko 産業医科大学, 医学部, 教授 (90362467)
• Co-Investigator(Kenkyū-buntansha): 東 みゆき 東京医科歯科大学, 医歯(薬)学総合研究科, 教授 (90255654)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: Ｔ細胞 / 樹状細胞 / 免疫学的記憶 / T細胞

Outline of Final Research Achievements

To visualize BATF expression during memory T cell formation, reporter gene constructs were generated by fusing the luciferase, GFP, or dsRED gene to the BATF promoter. Interestingly, BATF expression was induced both in T cells and dendritic cells upon stimulation. While BATF expression was induced upon TLR stimulation in murine dendritic cells derived from spleen and bone marrow, BATF was constitutively expressed in dendritic cell lines, suggesting that mechanisms regulating BATF expression in primary cells and cell lines may be different. These results suggest that it might be important to establish the reporter system using primary cells for the analysis of molecular mechanisms regulating BATF-expression.

Research Products

• [Presentation] SIRT1とT細胞分化 (2015)
  • Author(s): 岩井 佳子
  • Organizer: 第15 回日本抗加齢医学会総会
  • Place of Presentation: 福岡国際会議場(福岡県福岡市)
  • Year and Date: 2015-05-29 – 2015-05-31
  • Invited