Elucidation of the metal dynamics of dental metal allergy

• Project/Area Number: 24592860
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Conservative dentistry
• Research Institution: Tohoku University
• Principal Investigator: Shigehito Higuchi 東北大学, 歯学研究科(研究院), 大学院非常勤講師 (10291262)
• Co-Investigator(Kenkyū-buntansha): OGASAWARA KOUETSU 東北大学, 加齢医学研究所, 教授 (30323603)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: アレルギー / アレルギー・ぜんそく / 金属アレルギー / 金属イオン / ニッケル

Outline of Final Research Achievements

Metal allergy is categorized in the delayed allergy, because the symptoms are developed at least 24 hours after contact with the metal. Elution of metal ion is known to be essential for the development of metal allergy. Although the metal ion might be bound to protein, the molecular mechanism is unknown. Accordingly, the present study is to reveal the dynamics of metal ions that cause allergies of metal. To clarify the kinetics of metal ions, we examined to visualize the nickel ions. We were able to develop the visualization of nichel ion by fluorescent dye. This visualization was realized and cell analyzer such as a fluorescence microscope or flow cytometry will be available. We were able to analyze the nickel at the cellular level uptake quantitatively and visually.

Research Products

• [Journal Article] NKG2D+ IFN-γ+ CD8+ T cells are responsible for palladium allergy. (2014)
  • Author(s): Kawano M, Nakayama M, Aoshima Y, Nakamura K, Ono M, Nishiya T, Nakamura S, Takeda Y, Dobashi A, Takahashi A, Endo M, Ito A, Ueda K, Sato N, Higuchi S, Kondo T, Hashimoto S, Watanabe M, Watanabe M, Takahashi T, Sasaki K, Nakamura M, Sasazuki T, Narushima T, Suzuki R, Ogasawara K.
  • Journal Title: PLoS One. Volume: 9 Issue: 2 Pages: 1-12
  • DOI: 10.1371/journal.pone.0086810
  • Peer Reviewed / Open Access
• [Presentation] 金属炎症・アレルギーの免疫学的解析 (2013)
  • Author(s): 小笠原康悦、川野光子、中村生、遠藤実里、武田裕利、樋口繁仁
  • Organizer: 日本バイオマテリアル学会東北地域講演会
  • Place of Presentation: 仙台
  • Invited
• [Book] 免疫抑制にはたらくドレスNK細胞の発見 (2013)
  • Author(s): 橋元亘, 中村恭平, 遠藤実里, 島田栄理遣, 小笠原康悦, 中山勝文, 佐藤直毅, 樋口繁仁
  • Publisher: 先端医学社
• [Book] 炎症と免疫 (2013)
  • Author(s): 橋元亘、中村恭平、遠藤実里、島田栄理遣、小笠原康悦、中山勝文、佐藤直毅、樋口繁仁
  • Publisher: 先端医学社