Molecular biological analysis of biomarkers for angiogenesis to promote dental pulp tissue engineering

• Project/Area Number: 24592862
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Conservative dentistry
• Research Institution: Niigata University
• Principal Investigator: KANEKO Tomoatsu 新潟大学, 医歯学総合病院, 助教 (70345297)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2012: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 歯髄再生 / 血管新生関連バイオマーカー

Outline of Final Research Achievements

Angiogenesis is one of important factors to engineer dental pulp tissue. However, it is still unknown how biomarkers of angiogenesis participate in pulp tissue regeneration. Thus, the roles of the biomarkers were analyzed by using immunohisitochemcal and molecular biological methods. The results suggested that VEGF and FGF were important factors to promote the tissue engineering.

Research Products

• [Journal Article] 幹細胞を用いた歯髄組織再生におけるマクロファージ様細胞について. (2015)
  • Author(s): 12)金子友厚
  • Journal Title: 日本歯内療法学会雑誌 Volume: 36 Pages: 13-16
  • NAID: 130006108659
  • Peer Reviewed
• [Journal Article] M2 macrophages participate in the biological tissue healing reaction to mineral trioxide aggregate (2014)
  • Author(s): Ito T, Kaneko T, Yamanaka Y, Shigetani Y, Yoshiba K, Okiji T
  • Journal Title: Journal of Endodontics Volume: 40 Issue: 3 Pages: 379-383
  • DOI: 10.1016/j.joen.2013.11.011
  • Peer Reviewed
• [Journal Article] Evaluation of the responses of MHC class II molecule-expressing cells and macrophages to epoxy resin-based and 4-META-containing, methacrylate resin-based root canal sealers in rat subcutaneous tissue (2013)
  • Author(s): Yamanaka Y, Shigetani Y, Yoshiba K, Kaneko T, Yoshiba N, Okiji T
  • Journal Title: Dental Materials Journal Volume: 32 Issue: 5 Pages: 822-827
  • DOI: 10.4012/dmj.2013-005
  • NAID: 130004519306
  • ISSN: 0287-4547, 1881-1361
  • Peer Reviewed
• [Journal Article] Up-regulation of p38 mitogen-activated protein kinase during pulp injury-induced glial cell/neuronal interaction in the rat thalamus. (2013)
  • Author(s): Kaneko T, Chokechanachaisakul U, Kawamura J, Yamanaka Y, Ito T, Sunakawa M, Suda H, Okiji T.
  • Journal Title: J Endod. Volume: 39 Issue: 4 Pages: 488-92
  • DOI: 10.1016/j.joen.2012.11.018
  • Peer Reviewed
• [Journal Article] Immunohistochemical and gene expression analysis of stem-cell-associated markers in rat dental pulp (2013)
  • Author(s): Kaneko T, Arayatrakoollikit U, Yamanaka Y, Ito T, Okiji T
  • Journal Title: Cell Tissue Res Volume: 351(3) Issue: 3 Pages: 425-32
  • DOI: 10.1007/s00441-012-1539-9
  • Peer Reviewed
• [Journal Article] Mineral trioxide aggregateに対するラット皮下結合組織の応答-マクロファージ関連分子の免疫組織化学的・分子生物学的解析-. (2013)
  • Author(s): 伊藤崇史、山中裕介、金子友厚、興地隆史.
  • Journal Title: 日本歯科保存学雑誌 Volume: 56 Pages: 9-16
  • NAID: 110009596776
  • Peer Reviewed
• [Journal Article] H. A novel whole tooth-in-jaw-bone culture of rat molars : morphological, immunohistochemical, and laser capture microdissection analysis (2012)
  • Author(s): Chokechanachaisakul U, Kaneko T, Yamanaka Y, Okiji T, Suda H
  • Journal Title: Microsc Res Tech Volume: 75(10) Issue: 10 Pages: 1341-7
  • DOI: 10.1002/jemt.22072
  • Peer Reviewed
• [Journal Article] Expression of angiogenic factors in rat periapical lesions (2012)
  • Author(s): Yusuke Yamanaka, Tomoatsu Kaneko, Kunihiko Yoshiba, Reika Kaneko, Nagako Yoshiba, Yoshimi Shigetani, Jacques E. Nor, Takashi Okiji
  • Journal Title: Journal of Endodontics Volume: 38(3) Issue: 3 Pages: 313-317
  • DOI: 10.1016/j.joen.2011.11.009
  • Peer Reviewed
• [Presentation] Suppression of astrocytic activation as antigen presenting cells in rat thalamus following dental pulp inflammation. (2014)
  • Author(s): Kawamura J, Kaneko T, Yamanaka Y, Ito T, Okiji T
  • Organizer: Dendritic Cell (DC) 2014
  • Place of Presentation: Loire Valley, France
  • Year and Date: 2014-09-14 – 2014-09-18
• [Presentation] Immnue-LCM analysis of M1/M2 macrophages in engineered dental pulp tissues. (2014)
  • Author(s): Kaneko T, Yamanaka Y, Ito T, Okiji T
  • Organizer: 18th International Microscopy Congress
  • Place of Presentation: Prague, Czech
  • Year and Date: 2014-09-07 – 2014-09-12
• [Presentation] 再生歯髄組織におけるマクロファージの活性化と成熟―血管内皮細胞混合移植の影響―. (2014)
  • Author(s): 金子友厚、伊藤崇史、末山有希子、山中裕介、吉羽邦彦、興地隆史.
  • Organizer: 第35回 日本歯内療法学会学術大会
  • Place of Presentation: 新潟県朱鷺メッセ
  • Year and Date: 2014-07-12 – 2014-07-13
• [Presentation] 歯科用実体顕微鏡を用いた髄腔および根管観察の歯内療法基礎実習への導入. (2014)
  • Author(s): 金子友厚, 前田健康, 興地隆史
  • Organizer: 第33回日本歯科医学教育学会.
  • Place of Presentation: 福岡県北九州国際会議場
  • Year and Date: 2014-07-04 – 2014-07-05
• [Presentation] 再生歯髄組織内マクロファージにおけるM1/M2関連遺伝子の発現. (2014)
  • Author(s): 金子友厚，山中裕介、伊藤崇史、吉羽邦彦、興地隆史.
  • Organizer: 日本歯科保存学会
  • Place of Presentation: 滋賀県立芸術劇場
  • Year and Date: 2014-06-19 – 2014-06-20
• [Presentation] ラット実験的歯髄炎における幹細胞関連遺伝子発現の経時的検索. (2014)
  • Author(s): 伊藤崇史、金子友厚、山中裕介、興地隆史.
  • Organizer: 日本歯科保存学会
  • Place of Presentation: 滋賀県立芸術劇場
  • Year and Date: 2014-06-19 – 2014-06-20
• [Presentation] Accumulation of M2 macrophages in mineral trioxide aggregate-implanted tissue. (2013)
  • Author(s): Takafumi Ito, Tomoatsu Kaneko, Yusuke Yamanaka, Kunihiko Yoshiba, Yoshimi Shigetani, Takashi Okiji.
  • Organizer: The 15th Joint Scientific Meeting between the Japanese Society of Conservative Dentistry and the Korean Academy of Conservative Dentistry.
  • Place of Presentation: Gyeongju, Korea.
• [Presentation] Analysis of Glial Cell-related Genes in Rat Thalamus following Pulpitis. (2013)
  • Author(s): Jun Kawamura, Tomoatsu Kaneko, Yusuke Yamanaka, Takafumi Ito, Mitsuhiro Sunakawa , Takashi Okiji , Hideaki Suda.
  • Organizer: IADR-ASIA.
  • Place of Presentation: Bangkok, Thailand.
• [Presentation] NF-κB-blockade increases Bax/Bcl-2 ratio and reduces angiogenesis in periapical lesions. (2013)
  • Author(s): Yamanaka Y, Kaneko T, Ito T, Okiji T.
  • Organizer: IADR-ASIA
  • Place of Presentation: Bangkok, Thailand.
• [Presentation] Immnue-LCM analysis of M1/M2 macrophages in engineered dental pulp tissues (2013)
  • Author(s): Tomoatsu Kaneko, Yusuke Yamanaka, Takafumi Ito, Takashi Okiji .
  • Organizer: The 55th Symposium of the Society for Histochemistry & Cost Nanonet.
  • Place of Presentation: チェコ(プラハ)
• [Presentation] 再生歯髄組織内におけるマクロファージの活性化と成熟 (2013)
  • Author(s): 金子友厚, 山中裕介, 伊藤崇史, 吉羽邦彦, 興地隆史.
  • Organizer: 日本顕微鏡学会
  • Place of Presentation: 愛知県産業労働センター
• [Presentation] 実験的歯髄炎はラット視床におけるミクログリアを活性化させる. (2013)
  • Author(s): 河村 隼，金子友厚，山中裕介，伊藤崇史，砂川光宏，興地隆史，須田英明.
  • Organizer: 日本歯科保存学会.
  • Place of Presentation: 福岡国際会議場.
• [Presentation] ラット切歯実験的歯髄炎が幹細胞関連遺伝子の発現に与える影響. (2013)
  • Author(s): 伊藤崇史、金子友厚、山中裕介、興地隆史.
  • Organizer: 日本歯科保存学会.
  • Place of Presentation: 福岡国際会議場
• [Presentation] Glial Cell/neuronal Interaction in Thalamus following Dental Pulp Inflammation. (2013)
  • Author(s): Jun Kawamura, Tomoatsu Kaneko, Uraiwan Chokechanachaisakul, Yusuke Yamanaka, Takafumi Ito, Mitsuhiro Sunakawa , Hideaki Suda, Takashi Okiji.
  • Organizer: IADR
  • Place of Presentation: Seattle, WA
• [Presentation] The roles of CD68 expressing macrophages in engineered dental pulp tissues. (2012)
  • Author(s): Tomoatsu Kaneko, Yusuke Yamanaka, Takashi Okiji.
  • Organizer: The 12th International Symposium on Dendritic Cells.
  • Place of Presentation: Daegu, Korea
• [Presentation] CD146 and MAP1B expressing dental pulp stem cell-like cells in rat dental pulp. (2012)
  • Author(s): Takafumi Ito, Yusuke Yamanaka, Tomoatsu Kaneko, Nagako Yoshiba, Kunihiko Yoshiba, Takashi Okiji.
  • Organizer: 54th Symposium of the Society for Histochemistry, Imaging Development, Tracking Cells in Order and Disorder.
  • Place of Presentation: Vienna, Austria
• [Presentation] The role of endothelial cells in macrophage differentiation in engineered dental pulp tissues. (2012)
  • Author(s): Tomoatsu Kaneko, Yusuke Yamanaka, Takashi Okiji.
  • Organizer: FDI
  • Place of Presentation: 香港
• [Presentation] Evaluation of biocompatible materials by using double-labeling immunohistochemical method. (2012)
  • Author(s): Takafumi Ito, Yusuke Yamanaka, Tomoatu Kaneko, Kunihiko Yoshiba, Nagako Yoshiba, Takshi Okiji.
  • Organizer: The 14th International Congress of Histochemistry and Cytochemistry
  • Place of Presentation: 京都
• [Presentation] Double immune labeling of CD146 and MAP1B expressing stem cell-like cells in rat dental pulp. (2012)
  • Author(s): Yusuke Yamanaka, Tomoatu Kaneko, Takahumi Ito, Kunihiko Yoshiba, Nagako Yoshiba, Takshi Okiji.
  • Organizer: The 14th International Congress of Histochemistry and Cytochemistry
  • Place of Presentation: 京都
• [Presentation] Immuno-laser capture microdissection analysis of dental pulp macrophages in a whole-tooth culture model. (2012)
  • Author(s): Uraiwan Chokechanachaisakul, Tomoatsu Kaneko, Yusuke Yamanaka, Mitsuhiro Sunakawa , Takashi Okiji , Hideaki Suda.
  • Organizer: The 14th International Congress of Histochemistry and Cytochemistry
  • Place of Presentation: 京都
• [Presentation] ラット実験的根尖性歯周炎の成立過程における血管新生関連遺伝子の発現. (2012)
  • Author(s): 山中裕介、金子友厚、吉羽邦彦、伊藤崇史、吉羽永子、重谷佳見、興地隆史.
  • Organizer: 新潟歯学会
  • Place of Presentation: 新潟
• [Presentation] Lipopolysaccharide-induced upregulation of antigen-presenting cell-related genes in macrophages in engineered-pulp. (2012)
  • Author(s): Tomoatsu Kaneko, Yusuke Yamanaka, Takafumi Ito, Takashi Okiji.
  • Organizer: JADR
  • Place of Presentation: 新潟
• [Presentation] p38 MAPK-upregulation/activation during pulp injury-induced glial-neuronal interaction in rat thalamus. (2012)
  • Author(s): J Kawamura, T Kaneko, C Uraiwan, Y Yamanaka, T Ito, M, Sunakawa, H Suda, T Okiji.
  • Organizer: JADR
  • Place of Presentation: 新潟
• [Presentation] Stem cell-related marker-expression in rat dental pulp and periodontal ligament. (2012)
  • Author(s): Y Yamanaka, T Kaneko, T Ito, K Yoshiba, N Yoshiba, T Okiji.
  • Organizer: JADR
  • Place of Presentation: 新潟
• [Presentation] Macrophage/dendritic cell responses to pulp-capping materials in rat subcutaneous. (2012)
  • Author(s): T Ito, T Kaneko, Y Yamanaka, K Yoshiba, N Yoshiba Y Shigetani, T Okiji.
  • Organizer: JADR
  • Place of Presentation: 新潟
• [Presentation] ラット実験的歯髄炎により生じる視床におけるphospho-p38 MAPKの発現に関する免疫組織学的検索. (2012)
  • Author(s): 河村隼，金子友厚，チョックチャナチャイサクン ウライワン，山中裕介，伊藤崇史，砂川光宏，興地隆史，須田英明.
  • Organizer: 日本歯科保存学会
  • Place of Presentation: 広島
• [Presentation] The expression of MAPK38 families in rat central nervous system is regulated by the signal of tooth pain. (2012)
  • Author(s): CHOKECHANACHAISAKUL Uraiwan, KANEKO Tomoatsu, SUNAKAWA Mitsuhiro, OKIJI Takashi, SUDA Hideaki.
  • Organizer: 日本歯科保存学会
  • Place of Presentation: 沖縄
• [Book] Understand Cancer - Research and Treatment (2015)
  • Author(s): Tomoatsu Kaneko, Takashi Okiji, Jacques E. Nor.
  • Publisher: iConcept Press
• [Book] Yearbook2014 今だからこそ押さえておきたい! 世界の歯内療法の潮流-第９回世界歯内療法会議の主な演題からー 再生歯髄組織内におけるマクロファージの活性化と成熟. (2014)
  • Author(s): 金子友厚, 山中裕介, 伊藤崇史, 吉羽邦彦, 興地隆史
  • Total Pages: 169
  • Publisher: クインテッセンス出版
• [Book] The Research and Biology of Cancer II. (2014)
  • Author(s): Tomoatsu Kaneko, Takashi Okiji, Jacques E. Nor.
  • Total Pages: 222
  • Publisher: iConcept Press
• [Book] Handbook of Macrophages: Life Cycle, Functions and Diseases. (2012)
  • Author(s): 2. Tomoatsu Kaneko, Yusuke Yamanaka, Takashi Okiji.
  • Total Pages: 434
  • Publisher: Nova Science Publishers
• [Remarks] 新潟大学研究者総覧: 金子 友厚
  • URL: http://researchers.adm.niigata-u.ac.jp/staff/?userId=100000348&lang=
• [Remarks] 新潟大学研究者総覧: 金子 友厚
  • URL: http://researchers.adm.niigata-u.ac.jp/staff/?userId=100000348&lang=