| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
The aim of this study was to elucidate cellular events during pulp tissue wound healing and reparative dentin formation after direct pulp capping. M2 macrophage-associated molecule-expressing cells transiently accumulated beneath the exposure site. The deposition of osteopontin and dentin matrix protein 1 (DMP1) at exposed pulp sites preceded the appearance of nestin-immunoreactive cells, active cell proliferation and new matrix formation. These suggest that M2 macrophages participate in the initial phases of the healing and that osteopontin and DMP1 act as a trigger of pulp repair. In addition, numerous α-smooth muscle actin (SMA)-positive cells emerged at the wound margin, and the initially formed mineralized barrier was lined with α-SMA-positive cells similar in appearance to reparative odontoblasts, some of which co-expressed nestin. Fibrillin-1 degradation and down-regulation might be implicated in the differentiation of α-SMA-positive cells in this process.
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