The development of direct pulp capping materials using bilogical agent

• Project/Area Number: 24592868
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Conservative dentistry
• Research Institution: Hiroshima University
• Principal Investigator: FUJII Masashi 広島大学, 大学病院, 講師 (10284217)
• Co-Investigator(Kenkyū-buntansha): SUZUKI Shigeki 広島大学, 大学院医歯薬学保健学研究院, 助教 (30549762)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 歯髄 / 直接覆髄 / 創傷治癒 / Wnt / 覆髄材

Outline of Final Research Achievements

Various cytokines and growth factors induce wound healing during inflammatory responses. Wnts are known to enhance skin wound healing. In this study, we utilized gene-modified mice in which Wnts signal is prolonged during inflammatory responses. We experimentally exposed the molars of these mice to evoke pulpal tissue inflammation to analyze the importance of Wnt signal during pulpal tissue inflammation. The wound healing responses of pulpal tissues in the molars of these gene-modified mice were enhanced compared with that of control mice. The regenerated hard tissues in these gene-modified mice were morphologically bone-like structure and immunohistochemically dentin-like tissues. These results indicated the development of methods to enhance endogenous Wnt signal in pulpal tissues could be contribute for generating biological materials for direct pulp capping.

Research Products

• [Journal Article] Wnt Acts as a Pro-Survival Signal to Enhance Dentin Regeneration. (2015)
  • Author(s): Hunter D.J., Bardet C., Mouraret S., Liu B., Singh G., Sadoine J., Dhamdhere G., Smith A., Tran X.V., Joy A., Rooker S., Suzuki S., Vuorinen A., Miettinen S., Chaussain C., and *Helms J.A.
  • Journal Title: J Bone Miner Res. Volume: in print Issue: 7 Pages: 1150-1159
  • DOI: 10.1002/jbmr.2444
  • Peer Reviewed / Open Access
• [Journal Article] Adhesive and Migratory Effects of Phosphophoryn Are Modulated by Flanking Peptides of the Integrin Binding Motif. (2014)
  • Author(s): Suzuki S, Kobuke S, Haruyama N, Hoshino H, Kulkarni AB, Nishimura F
  • Journal Title: PLoS One Volume: 9(11) Issue: 11 Pages: e112490-e112490
  • DOI: 10.1371/journal.pone.0112490
  • Peer Reviewed / Open Access
• [Journal Article] The Effects of Matrix Trioxide Aggregate (MTA) on the Adhesion, Migration and Apoptosis of Dental Pulp Cells (2014)
  • Author(s): Suzuki, S., Nagayasu, S., Arakawa, M., Kobuke, S., Hoshino, H., Hashikata, T., Motoyama, T., and Nishimura, F.
  • Journal Title: The Japanese Journal of Conservative Dentistry Volume: 57 Issue: 6 Pages: 547-554
  • DOI: 10.11471/shikahozon.57.547
  • NAID: 130005005966
  • ISSN: 0387-2343, 2188-0808
  • Peer Reviewed
• [Journal Article] The Inflammation-lipocalin2 axis may contribute to the development of chronic kidney disease. (2014)
  • Author(s): Hashikata, A., Yamashita, A., Suzuki, S., Nagayasu, S., Shinjo, T., Taniguchi, A., Fukushima, M., Nakai, Y., Nin, K., Watanabe, N., Asano, T., Abiko, Y., Kushiyama, A., Nagasaka, S., and *Nishimura, F.
  • Journal Title: Nephrol Dial Transplant. Volume: 29 Pages: 611-8
  • Peer Reviewed
• [Presentation] Dentin sialophosphoproteinの開裂がその機能発現に及ぼす影響の検討 (2012)
  • Author(s): 小武家誠司、鈴木茂樹、藤井理史、西村英紀
  • Organizer: 日本歯科保存学会
  • Place of Presentation: 広島
• [Presentation] 内在性Wntによる歯髄創傷治癒促進効果とDentin Phosphoproteinによる硬組織形成作用の検討
  • Author(s): 鈴木茂樹
  • Organizer: 日本歯科保存学会
  • Place of Presentation: 福岡国際会議場
  • Invited