Control analysis of collagen post-translational modifications on natural bioactive peptide fractions in a preosteoblastic cell culture system
Project/Area Number |
24592875
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Conservative dentistry
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Research Institution | Nagasaki University |
Principal Investigator |
YAMADA Shizuka 長崎大学, 医歯薬学総合研究科(歯学系), 准教授 (00363458)
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Project Period (FY) |
2012-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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Keywords | 魚由来コラーゲンペプチド / MC3T3-E1細胞 / コラーゲン翻訳後修飾 / LOX / LH1 / LH2 / MALDI-TOF / リアルタイムPCR / 魚コラーゲンペプチド / 分子ふるいクロマトグラフィー / 飛行時間型質量分析法 / フィッシュコラーゲンペプチド / 骨芽細胞 / 石灰化 / ウェスタンブロット分析 |
Outline of Final Research Achievements |
Fish collagen peptides (FCP) were distributed into fractions I~III using the molecular sieve chromatography to investigate the effects on collagen post-translational modification-related enzymes in preosteoblasts by the difference between a molecular weight (MW) of FCP. In TOF-MS, MWs of fractions I, II and III were proved to be 2.9k~3.2kDa, 2.4~2.8kDa and 1.8k~2.3kDa, respectively. Although a cell number of fraction III group was significantly lower than that of a control group in the 2, 5, 7 days of cell culture, there was no difference between each group in 10 and 14 days of cell culture. LH2 mRNA expression did not have a significant difference between each group. However, LH1 and LOX mRNA expressions in fraction III group were significantly higher than those in other groups (p<0.01). These results suggested that a smaller MW of FCP could have a positive effect on the collagen cross-linking in preosteoblasts.
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Report
(4 results)
Research Products
(12 results)
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[Journal Article] Biological safety of fish (tilapia) collagen2014
Author(s)
Kohei Yamamoto, Kazunari Igawa, Kouji Sugimoto, Yuu Yoshizawa, Kajiro Yanagiguchi, Takeshi Ikeda, Shizuka Yamada and Yoshihiko Hayashi
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Journal Title
BioMed Research International
Volume: 2014
Pages: 1-9
DOI
NAID
Related Report
Peer Reviewed / Open Access
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