| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Outline of Final Research Achievements |
Fish collagen peptides (FCP) were distributed into fractions I~III using the molecular sieve chromatography to investigate the effects on collagen post-translational modification-related enzymes in preosteoblasts by the difference between a molecular weight (MW) of FCP. In TOF-MS, MWs of fractions I, II and III were proved to be 2.9k~3.2kDa, 2.4~2.8kDa and 1.8k~2.3kDa, respectively. Although a cell number of fraction III group was significantly lower than that of a control group in the 2, 5, 7 days of cell culture, there was no difference between each group in 10 and 14 days of cell culture. LH2 mRNA expression did not have a significant difference between each group. However, LH1 and LOX mRNA expressions in fraction III group were significantly higher than those in other groups (p<0.01). These results suggested that a smaller MW of FCP could have a positive effect on the collagen cross-linking in preosteoblasts.
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