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Elucidation of mechanism on dentin hypersensitivity; cell response by hypertonic stress.

Research Project

Project/Area Number 24592876
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Conservative dentistry
Research InstitutionKagoshima University

Principal Investigator

MASAYUKI Tokuda  鹿児島大学, 医歯(薬)学総合研究科, 准教授 (20253891)

Co-Investigator(Kenkyū-buntansha) MORIMOTO Yoko  鹿児島大学, 医学部・歯学部附属病院, 助教 (30437967)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords象牙質知覚過敏症 / 象牙芽細胞 / スクロース / 高浸透圧 / 細胞死 / 分裂促進因子活性化タンパク質キナーゼ / キシリトール / TRPV1 / AQP2 / 浸透圧 / 細胞内転写因子
Outline of Final Research Achievements

Osmotic stress is one cause of dental pain due to caries or dentin hypersensitivity. The mechanism of osmotic-induced dental pain is not completely understood. The purpose of this study was to examine the responses of odontoblasts under sucrose-induced hyperosmotic stress. Cell viability decreased over 700 mOsm for 3 h cell culture. The shapes of cells and nuclei became irregular and vacuolar under hyperosmotic stress. The expression of cleaved caspase-3 was increased after treatment with hyperosmotic stress. Flow cytometry analysis detected no annexin V-positive cells, indicating apoptosis. Three MAPKs phosphorylation were induced by hyperosmotic stress. Inhibitors of three MAPKs inhibited the hyperosmotic stress-induced decline in cell cultures at 500 and 700 mOsm. Hyperosmotic stress induces cell death of OLCs with sucrose through a MAPK pathway.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (4 results)

All 2015 2014 2013 2012

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (2 results)

  • [Journal Article] Involvement of TRPV1 and AQP2 in hypertonic stress by xylitol in odontoblast cells.2015

    • Author(s)
      35.Tokuda M*, Fujisawa M, Kawakami Y, Morimoto-Yamashita Y, Torii M
    • Journal Title

      Connect Tissue Res.

      Volume: 56 Issue: 1 Pages: 44-49

    • DOI

      10.3109/03008207.2014.984804

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Hyperosmotic stress induces cell death in an odontoblast-lineage cell line2012

    • Author(s)
      M Fujisawa, M Tokuda, Y Morimoto-Yamashita, S Tatsuyama, S Arany, T Sugiyama, C Kitamura, Y Shibukawa, M Torii
    • Journal Title

      Journal of Endodontics

      Volume: 38 Issue: 7 Pages: 931-935

    • DOI

      10.1016/j.joen.2012.03.023

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] 象牙芽細胞培養系におけるキシリトールによる高浸透圧刺激の影響2014

    • Author(s)
      徳田雅行,川井絢矢,作田哲也,小山徹,宮下桂子,藤澤真理,江本真規子,森元陽子,鳥居光男,松下健二
    • Organizer
      日本歯科保存学会秋季大会
    • Place of Presentation
      山形市(山形テルサ)
    • Year and Date
      2014-10-30 – 2014-10-31
    • Related Report
      2014 Annual Research Report
  • [Presentation] 象牙芽細胞培養系におけるキシリトールによる高浸透圧刺激の影響2013

    • Author(s)
      徳田雅行、藤澤真理、川上克子、江本真規子、鳥居光男
    • Organizer
      日本歯科保存学会
    • Place of Presentation
      秋田県総合生活文化会館
    • Related Report
      2013 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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