A study of intentional replamtation using with three-dimensional reconstructed organotypic culture of rat periodontal cells

• Project/Area Number: 24592886
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Conservative dentistry
• Research Institution: The Nippon Dental University
• Principal Investigator: IGARASHI MASARU 日本歯科大学, 新潟生命歯学部, 教授 (90168104)
• Co-Investigator(Kenkyū-buntansha): KITAJIMA Kayoko 日本歯科大学, 新潟生命歯学部, 准教授 (00177841) ; ARAI Kyoko 日本歯科大学, 新潟生命歯学部, 講師 (10434143)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 歯根膜 / 再生医学 / 三次元培養 / 再植法 / 抜歯窩 / 歯根 / セメント質 / 移植 / 歯根膜細胞 / コラーゲンゲル / 再生医療 / 脱臼歯 / 再植 / 歯槽骨

Outline of Final Research Achievements

This investigation showed the procedures for extraction of rat upper molar and the healing process after intentional implantation of the tooth with normal periodontal ligament. The periodontal tissues were obtained from the molar tooth and used for the primary culture. The largest root was cut off and the dental pulp and periodontal tissue were removed. The root was cut into the 4 pieces at longitudinal plane and 1mm length. Two weeks later, the piece was replanted to the previous tooth socket. The animals were sacrificed after 2 and 4 weeks and the decalcified paraffin sections were prepared. The sections were stained with hematoxylin-eosin and observed. The sections showed that the resorptions of the root surface appeared at 2 and 4 weeks. However the inflammations were not seen on any experimental groups. The type of the tissue healings were fibrous scar healing not osseous healing.