Research and development of a novel denture cleaning device utilizing a disinfection technique based on photolysis of hydrogen peroxide

• Project/Area Number: 24592901
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Prosthetic dentistry
• Research Institution: Tohoku University
• Principal Investigator: KANNO Taro 東北大学, 歯学研究科(研究院), 助教 (30302160)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: ヒドロキシルラジカル / 義歯洗浄 / 過酸化水素 / 可視光照射

Outline of Final Research Achievements

In the present study, the bactericidal effect and influence on the denture base-resin of denture cleaning technique using a newly developed device based on photolysis of hydrogen peroxide were evaluated. The device consisted of ultrasound cleaning bath and high power LED. Bactericidal test showed that the photolysis of hydrogen peroxide killed Streptococcus mutans with a >5-log reduction of viable counts within 3 min. Regarding the influence of the cleaning technique on the resin material, it was found that the strength and color were affected as the irradiance of LED increased. However, the results suggest that the influence may be acceptable when the LED was used at an irradiance of 10 mW/cm2 even on the assumption of repeated denture cleanings.

Research Products

• [Journal Article] Synergistic interaction between wavelength of light and concentration of H2O2 in bactericidal activity of photolysis of H2O2. (2015)
  • Author(s): Toki, T., Nakamura, K., Kurauchi, M., Kanno, T., Katsuda, Y., Ikai, H., Hayashi, E., Egusa, H., Sasaki, K., Niwano, Y.
  • Journal Title: Journal of Bioscience and Bioengineering Volume: 19(3) Issue: 3 Pages: 1358-1362
  • DOI: 10.1016/j.jbiosc.2014.08.015
  • NAID: 210000004988
  • Peer Reviewed
• [Journal Article] Postantifungal-like effect of sublethal treatment of Candida albicans with acid-electrolyzed water. (2015)
  • Author(s): Mokudai T, Kanno T, Niwano Y.
  • Journal Title: Arch Oral Biol. Volume: 60 Issue: 3 Pages: 479-487
  • DOI: 10.1016/j.archoralbio.2014.12.008
  • Peer Reviewed
• [Journal Article] Involvement of reactive oxygen species in the cytotoxic effect of acid-electrolyzed water. (2015)
  • Author(s): Mokudai T, Kanno T, Niwano Y.
  • Journal Title: J Toxicol Sci. Volume: 40 Pages: 13-19
  • NAID: 130004904021
  • Peer Reviewed
• [Journal Article] Bactericidal Activity and Mechanism of Photoirradiated Polyphenols against Gram-Positive and -Negative Bacteria. (2015)
  • Author(s): Nakamura K, Ishiyama K, Sheng H, Ikai H, Kanno T, Niwano Y.
  • Journal Title: J Agric Food Chem. Volume: 印刷中 Issue: 35 Pages: 7707-7713
  • DOI: 10.1021/jf5058588
  • Peer Reviewed
• [Journal Article] Fracture resistance of monolithic zirconia molar crowns with reduced thickness. (2015)
  • Author(s): Nakamura K, Harada A, Inagaki R, Kanno T, Niwano Y, Milleding P, Örtengren U.
  • Journal Title: Acta Odontol Scand. Volume: 30 Issue: 2 Pages: 1-7
  • DOI: 10.1111/eos.12173
  • Peer Reviewed
• [Journal Article] Cytoprotective effect of short-term pretreatment with proanthocyanidin on human gingival fibroblasts exposed to harsh environmental conditions. (2014)
  • Author(s): Kurauchi M, Niwano Y, Shirato M, Kanno T, Nakamura K, Egusa H, Sasaki K.
  • Journal Title: PLoS One Volume: 9 Issue: 11 Pages: e113403-e113403
  • DOI: 10.1371/journal.pone.0113403
  • Peer Reviewed
• [Journal Article] Acceleration of proliferative response of mouse fibroblasts by short-time pretreatment with polyphenols. (2014)
  • Author(s): Tsuruya, M., Niwano, Y., Nakamura, K., Kanno, T., Nakashima, T., Egusa, H., Sasaki, K.
  • Journal Title: Applied Biochemistry and Biotechnology. Volume: 174(6) Issue: 6 Pages: 2223-2235
  • DOI: 10.1007/s12010-014-1124-7
  • Peer Reviewed
• [Journal Article] Blue light injures corneal epithelial cells in the mitotic phase in vitro. (2014)
  • Author(s): Niwano Y, Kanno T, Iwasawa A, Ayaki M, Tsubota K
  • Journal Title: Br J Ophthalmol. Volume: in press Issue: 7 Pages: 990-992
  • DOI: 10.1136/bjophthalmol-2014-305205
  • Peer Reviewed
• [Journal Article] Postantibiotic effect of disinfection treatment by photolysis of hydrogen peroxide. (2014)
  • Author(s): Odashima, Y., Nakamura, K., Ikai, H., Kanno, T., Meirelles, L., Sasaki, K., Niwano, Y.
  • Journal Title: Journal of Chemotherapy Volume: 26(2): Issue: 2 Pages: 92-100
  • DOI: 10.1179/1973947813y.0000000114
  • Peer Reviewed
• [Journal Article] In vitro evaluation of the risk of inducing bacterial resistance to disinfection treatment with photolysis of hydrogen peroxide (2013)
  • Author(s): Ikai, H., Odashima, Y., Kanno, T., Nakamura, K., Shirato, M., Sasaki, K., Niwano, Y
  • Journal Title: PLoS ONE Volume: 8(11) Issue: 11 Pages: e81316-e81316
  • DOI: 10.1371/journal.pone.0081316
  • Peer Reviewed
• [Journal Article] Synergistic effect of proanthocyanidin on the bactericidal action of the photolysis of H2O2. (2013)
  • Author(s): Ikai H, Nakamura K, Kanno T, Shirato M, Meirelles L, Sasaki K, Niwano Y.
  • Journal Title: Biocontrol Sci. Volume: 18(3) Pages: 137-141
  • NAID: 10031202904
  • Peer Reviewed
• [Journal Article] Influence of a new denture cleaning technique based on photolysis of H(2)O(2) the mechanical properties and color change of acrylic denture base resin. (2013)
  • Author(s): Nakahara T, Harada A, Yamada Y, Odashima Y, Nakamura K, Inagaki R, Kanno T, Sasaki K, Niwano Y.
  • Journal Title: Dent Mater J. Volume: 32(4) Pages: 529-536
  • Peer Reviewed
• [Journal Article] In vitro bactericidal activity of photo-irradiated oxydol products via hydroxyl radical generation. (2013)
  • Author(s): Oyamada A, Ikai H, Nakamura K, Hayashi E, Kanno T, Sasaki K, Niwano Y.
  • Journal Title: Biocontrol Sci. Volume: 18(2) Pages: 83-88
  • NAID: 10031184509
  • Peer Reviewed
• [Journal Article] Photo-irradiation of proanthocyanidin as a new disinfection technique via reactive oxygen species formation (2013)
  • Author(s): Nakamura K, Shirato M, Ikai H, Kanno T, Sasaki K, Kohno M, Niwano Y.
  • Journal Title: PLoS ONE Volume: 8 Issue: 3 Pages: e60053-e60053
  • DOI: 10.1371/journal.pone.0060053
  • Peer Reviewed
• [Journal Article] Synergistic Effect of Proanthocyanidin on Bactericidal Action of the Photolysis of H2O2 (2013)
  • Author(s): Hiroyo Ikai, Keisuke Nakamura, Taro Kanno, Midori Shirato, Luiz Meirelles
  • Journal Title: Bioscience Control Volume: in press
  • NAID: 10031202904
  • Peer Reviewed
• [Journal Article] In Vitro Bactericidal Activity on Photo-irradiated Oxydol Products via Hydroxyl Radical Generation (2013)
  • Author(s): Akira Oyamada, Hiroyo Ikai, Keisuke Nakamura, Eisei Hayashi, Taro Kanno, Keiichi Sasaki, Yoshimi Niwano
  • Journal Title: Bioscience Control Volume: in press
  • NAID: 10031184509
  • Peer Reviewed
• [Journal Article] ラジカル殺菌技術の歯科応用 (2013)
  • Author(s): 菅野太郎、中村圭祐
  • Journal Title: 歯界展望 Volume: 121(3) Pages: 514-521
• [Journal Article] Corrosive effect of disinfection solution containing hydroxyl radicals generated by photolysis of H₂O₂ on dental metals (2012)
  • Author(s): Nakamura K, Yamada Y, Takada Y, Mokudai T, Ikai H, Inagaki R, Kanno T, Sasaki K, Kohno M, Niwano Y
  • Journal Title: Dental Materials Journal Volume: 31 Issue: 6 Pages: 941-946
  • DOI: 10.4012/dmj.2012-098
  • NAID: 10031126072
  • ISSN: 0287-4547, 1881-1361
  • Peer Reviewed
• [Journal Article] Presence of hydrogen peroxide a source of hydroxyi radicals in acid electrolyzed water (2012)
  • Author(s): Mokudai T, Nakamura K, Kann T, Niwano Y
  • Journal Title: PLoS ONE Volume: 7 Issue: 9 Pages: e46392-e46392
  • DOI: 10.1371/journal.pone.0046392
  • Peer Reviewed
• [Journal Article] Bactericidal Action of Photoirradiated Gallic Acid via Reactive Oxygen Species Formation (2012)
  • Author(s): Nakamura K, Yamada Y, Ikai H, Kanno T, Sasaki K, Niwano Y.
  • Journal Title: J Agric Food Chem Volume: 60 Pages: 10048-10054
  • Peer Reviewed
• [Journal Article] Novel denture-cleaning system based on hydroxyl radical disinfection. (2012)
  • Author(s): Kanno T, Nakamura K, Ikai H, Hayashi E, Shirato M, Mokudai T, Iwasawa A, Niwano Y, Kohno M, Sasaki K.
  • Journal Title: Int J Prosthdont Volume: 25(4) Pages: 376-380
  • Peer Reviewed
• [Journal Article] In vitro and in vivo anti-Staphylococcus aureus activities of a new disinfection system utilizing photolysis of hydrogen peroxide (2012)
  • Author(s): Hayashi, E., Mokudai, T., Yamada, Y., Nakamura, K., Kanno, T., Sasaki, K., Niwano, Y
  • Journal Title: J. Biosci. Bioeng Volume: 114(2) Issue: 2 Pages: 193-197
  • DOI: 10.1016/j.jbiosc.2012.03.020
  • NAID: 110009495428
  • Peer Reviewed
• [Journal Article] Topical treatment of oral cavity and wounded skin with a new disinfection system utilizing photolysis of hydrogen peroxide in rats. (2012)
  • Author(s): Yamada Y, Mokudai T, Nakamura K, Hayashi E, Kawana Y, Kanno T, Sasaki K, Niwano Y.
  • Journal Title: J Toxicol Sci Volume: 37(2) Pages: 329-335
  • NAID: 10030126757
  • Peer Reviewed
• [Presentation] 過酸化水素の光分解および水の超音波分解を併用したヒドロキシルラジカル生成系の殺菌効果 (2014)
  • Author(s): 生宏、中村圭祐、菅野太郎、佐々木啓一、庭野吉己
  • Organizer: 日本防菌防黴学会第41回年次大会
  • Place of Presentation: 東京
  • Year and Date: 2014-09-25 – 2014-09-26
• [Presentation] 過酸化水素光分解殺菌技術を応用した新規歯周病治療器の安全性と有効性の検証 (2014)
  • Author(s): 猪飼紘代，目代貴之，中村圭祐，菅野太郎，庭野吉己
  • Organizer: 日本防菌防黴学会第41回年次大会
  • Place of Presentation: 東京
  • Year and Date: 2014-09-25 – 2014-09-26
• [Presentation] 活性酸素 (2014)
  • Author(s): 中村圭祐，菅野太郎，猪飼紘代，庭野吉己
  • Organizer: 日本防菌防黴学会第41回年次大会
  • Place of Presentation: 東京
  • Year and Date: 2014-09-25 – 2014-09-26
• [Presentation] 過酸化水素光分解殺菌技術における光の波長と過酸化水素濃度の相乗作用 (2014)
  • Author(s): 倉内美智子，唐木英俊，中村圭祐，菅野太郎，勝田悠介，猪飼紘代，林栄成，佐々木啓一，庭野吉己
  • Organizer: 日本防菌防黴学会第41回年次大会
  • Place of Presentation: 東京
  • Year and Date: 2014-09-25 – 2014-09-26
• [Presentation] Candida albicansに対する希釈酸性電解水のpost-antifungal like effectと活性酸素の関与 (2014)
  • Author(s): 目代貴之，菅野太郎，庭野吉己
  • Organizer: 日本防菌防黴学会第41回年次大会
  • Place of Presentation: 東京
  • Year and Date: 2014-09-25 – 2014-09-26
• [Presentation] 酸性電解水の細胞毒性における活性酸素の関与 (2014)
  • Author(s): 庭野吉己，目代貴之，追泉里実，菅野太郎
  • Organizer: 日本防菌防黴学会第41回年次大会
  • Place of Presentation: 東京
  • Year and Date: 2014-09-25 – 2014-09-26
• [Presentation] Bactericidal effect of photolysis of H2O2 in combination with sonolysis of water via hydroxyl radical generation (2014)
  • Author(s): Sheng, H., Nakamura, K., Kanno, T., Sasaki, K., Niwano, Y.
  • Organizer: 5th International Symposium for Interface Oral Health Science
  • Place of Presentation: 仙台
• [Presentation] Acceleration of proliferative response of 3T3-L1 mouse fibroblasts and human gingival fibroblasts by short-term exposure to proanthocyanidin (2014)
  • Author(s): Kurauchi, M., Nakamura, K., Kanno, T., Shirato, M., Sasaki, K., Niwano, Y.
  • Organizer: 5th International Symposium for Interface Oral Health Science
  • Place of Presentation: 仙台
• [Presentation] 過酸化水素光分解殺菌技術を応用した新規歯周病治療器の開発 (2013)
  • Author(s): 菅野太郎、中村圭祐、猪飼紘代、庭野吉己、佐々木啓一
  • Organizer: 日本歯周病学会2013春季学術大会（第56回）
  • Place of Presentation: 東京
• [Presentation] 歯垢染色剤に含まれる各種キサンテン系色素を光感受性物質とした一重項酸素依存性光線力学殺菌療法の比較 (2013)
  • Author(s): 庭野吉己、石山希里香、中村圭祐、猪飼紘代、菅野太郎、佐々木啓一
  • Organizer: 第23回日本光線力学学会学術講演会
  • Place of Presentation: 旭川
• [Presentation] Advantages of new disinfection treatment utilizing photolysis of hydrogen peroxide (2013)
  • Author(s): Ikai, H., Odashima, Y., Nakamura, K., Shirato, M., Kanno, T., Sasaki, K., Niwano, Y
  • Organizer: Innovative Research for Biosis-Abiosis Intelligent Interface Summer Seminaｒ
  • Place of Presentation: 宮城蔵王
• [Presentation] Bactericidal action of photogenerated singlet oxygen from photosensitizers used in plaque disclosing agents (2013)
  • Author(s): Ishiyama, K., Nakamura, K., Ikai, H., Kanno, T., Sasaki, K., Niwano, Y.
  • Organizer: Innovative Research for Biosis-Abiosis Intelligent Interface Summer Seminaｒ
  • Place of Presentation: 宮城蔵王
• [Presentation] 電気分解で生成された酸性電解水中に存在する水酸化ラジカルは殺菌活性に寄与するか？ (2013)
  • Author(s): 目代貴之、中村圭祐、菅野太郎、庭野吉己
  • Organizer: 日本防菌防黴学会第40回年次大会
  • Place of Presentation: 大阪
• [Presentation] 過酸化水素光分解殺菌法の耐性誘導試験 (2013)
  • Author(s): 猪飼紘代 、中村圭祐、小田島優、白土翠、菅野太郎、庭野吉己
  • Organizer: 日本防菌防黴学会第40回年次大会
  • Place of Presentation: 大阪
• [Presentation] 過酸化水素光分解殺菌法の短時間処理が残存細菌に及ぼす増殖抑制効果 (2013)
  • Author(s): 小田島優、中村圭祐、猪飼紘代、菅野太郎、佐々木啓一、庭野吉己
  • Organizer: 日本防菌防黴学会第40回年次大会
  • Place of Presentation: 大阪
• [Presentation] 各種ポリフェノールに対する青色可視光照射で得られる殺菌効果の比較検討 (2013)
  • Author(s): 中村圭祐、石山希里香、猪飼紘代、菅野太郎、庭野吉己
  • Organizer: 日本防菌防黴学会第40回年次大会
  • Place of Presentation: 大阪
• [Presentation] New Denture-Cleaning Device Utilizing Hydroxyl Radical Disinfection (2012)
  • Author(s): Taro Kanno, Keisuke Nakamura, Hiroyo Ikai, Midori Shirato, Takayuki Mokudai, Yoshimi Niwano and Keiichi Sasaki
  • Organizer: International Workshop on Biomaterials in Biosis-Abiosis Intelligent Interface Science
  • Place of Presentation: 宮城・蔵王
• [Presentation] 市販オキシドールの光分解殺菌効果 (2012)
  • Author(s): 小山田晃、猪飼紘代、中村圭祐、菅野太郎、佐々木啓一、庭野吉己
  • Organizer: 日本防菌防黴学会 第39回年次大会
  • Place of Presentation: 東京
• [Presentation] 過酸化水素光分解殺菌法に対する熱エネルギーの相乗的効果 (2012)
  • Author(s): 白土翠、猪飼紘代、中村圭祐、菅野太郎、佐々木啓一、庭野吉己
  • Organizer: 第22回日本歯科医学会総会
  • Place of Presentation: 大阪
• [Book] CollectedEvidence: Case Reports コレクテッド エビデンス vol.3 (2013)
  • Author(s): 弘岡秀明、菅野太郎
  • Total Pages: 201
  • Publisher: デンタルダイヤモンド社