| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Outline of Final Research Achievements |
Dental treatment often requires management of inflammation. The inflammation is closely associated with oxidative stress which hinders and delays the healing process. However, therapeutic approach focused on controlling oxidative stress in dental treatment is seldom found. This study tested the protective potential of an anti-oxidant, amino-acid derivative (AAD), in controlling the oxidative stress against TMJ chondrocytes. The inflammatory codition was simulated by adding hydrogen peroxide (H2O2) into culture medium. The exposure of H2O2 decreased the number of the cells, and both aggrecan and collagen II gene expressions. AAD addition into the H2O2 cultures restored the number of the cells, and the gene expression and glycosaminoglycan deposition to the level equivalent to untreated control culture. However, more importantly, the addition of AAD into the damaged culture restores their proliferative and differentiation capacities even under oxidative stress.
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