| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Outline of Final Research Achievements |
It is known that over 60% of patients with taste dysfunction are originally caused by zinc deficiency. Carbonic anhydrase (CA)Ⅵ, identical to gustin as a zinc-binding metalloprotein in human saliva, has significant relation to taste dysfunction. We have developed a swift and simple new diagnostic method using the immunochromatographic analysis. In this experiment, we examined a relationship between CA6 value and serum zinc level by using parotid saliva of patients with taste dysfunction, measured by the immunochromatographic method. We recognized a slight positive correlation between immunochromatographic measurement value. Furthermore, we attempted to apply the monoclonal antibody. Only LS-C196794 monoclonal antibody indicated comparable chromogenic reaction to polyclonal antibody we previously presented. Those results suggest that the measurement of zinc-binding protein in parotid saliva might be helpful to establish newly rapid diagnosis of zinc deficiency taste dysfunction.
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