Establishment of Peri-implantitis treatment using injection of advanced platelet rich plasma/cells complex and photodynamic therapy.

• Project/Area Number: 24592961
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Dental engineering/Regenerative dentistry
• Research Institution: Kagoshima University
• Principal Investigator: MATSUYAMA TAKASHI 鹿児島大学, 医歯(薬)学総合研究科, 准教授 (40253900)
• Co-Investigator(Kenkyū-buntansha): YOSHIMOTO Takehiko 鹿児島大学, 医学部・歯学部附属病院, 助教 (60419653) ; MACHIGASHIRA Miho 鹿児島大学, 医学部・歯学部附属病院, 講師 (80253897)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: インプラント周囲炎 / 光殺菌法 / GDF-5 / 多血小板血漿 / 骨再生 / rhGDF-5 / 下顎骨膜由来細胞 / 骨芽細胞用細胞 / 骨形成能 / 脱感染 / 骨芽細胞 / オステオカルシン

Outline of Final Research Achievements

The purpose of the present study was to evaluate cell biocompatibility and decontamination effect using photodynamic therapy and GDF-5/platelet-rich plasma(PRP)/mandibular periosteal cells complex. Photodynamic therapy on contaminated titanium surfaces led to decontamination without alteration of characterization on them. GDF-5/PRP complex enhanced activity of alkaline　phosphatase(ALP) in periosteal cells derived from rat mandibular compared to PRP alone stimulation. Furthermore, GDF-5/PRP complex induced the calcification of these cells on 14 days. These results suggest that GDF-5/PRP complex after photodynamic therapy on the contaminated titanium has an effect to induce bone regeneration.

Research Products

• [Journal Article] Cleavage of host cytokeratin-6 by lysine-specific gingipain induces gingival inflammation in periodontitis patients. (2015)
  • Author(s): Salunya Tancharoen, Takashi Matsuyama Koichi Kawahara, Miho Machigashira, Takashisa Arimura, Jan Plotempa, Yuko Nawa, Fumiyo Masuda, Miyacho Takahashi, Yoko Oyama, Chesta Binta, Teruo Hashiguchi, Ikuro Maruyama
  • Journal Title: PLoS One Volume: 17
  • Peer Reviewed / Open Access
• [Presentation] ベニアグラフト術を応用したインプラントの長期経過２症例 (2013)
  • Author(s): 松山孝司, 松井竜太郎, 川本真一郎, 西村正宏, 野口和行
  • Organizer: 日本口腔インプラント学会 近畿・北陸支部学術大会学術大会
  • Place of Presentation: 神戸
• [Presentation] Porphynomonas gingivalis ジンジパインはヒト歯肉上皮細胞からIL-33を誘導する (2012)
  • Author(s): 多田浩之、松山孝司、野口和行、島内英俊、松下健二
  • Organizer: 第55回秋季日本歯周病学会学術大会
  • Place of Presentation: つくば
• [Book] ザ・ペリオドントロジー (2014)
  • Author(s): 編集；和泉雄一、他 第１章７：松山孝司、野口和行
  • Total Pages: 279
  • Publisher: 永末書店