|Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|Outline of Final Research Achievements
The purpose of this study was generation of iPS-derived mesenchymal stem cells (MSC). However, we couldn't generate MSC from iPS cells. So we used the MSC to develop a new periodontal regenerative therapy.
Many researches has reported to the important role of enamel matrix derivative (EMD) in periodontal wound healing. Enamel matrix proteins provide an initial and essential step in the formation of a cellular cementum. We attempted to induce the cementum on the titanium implant by using the EMD.
EMD was immobilized by tresyl chloride-activation technique. MSC were seeded on the different titanium surfaces. The titanium surface with EMD immobilization (Ti-EMD) showed the significantly higher VEGF production of MSC than normal titanium surface. We observed that Ti-EMD stimulated a significant increase in the expression of CEMP1 mRNA in MSC compared with the control. Furthermore, EMD induced the production of CEMP1 protein in MSC. The mineralization was also confirmed by EMD stimulus.