Antioxidant and osteogenic properties on a modified titanium implant

• Project/Area Number: 24592966
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Dental engineering/Regenerative dentistry
• Research Institution: Showa University
• Principal Investigator: SHIBATA Yo 昭和大学, 歯学部, 助教 (30327936)
• Co-Investigator(Kenkyū-buntansha): MIYAZAKI Takashi 昭和大学, 歯学部, 教授 (40175617) ; MISHIMA Kenji 昭和大学, 歯学部, 教授 (50275343) ; YAMADA Atsushi 昭和大学, 歯学部, 講師 (50407558) ; YOSHIDA Misato 昭和大学, 歯学部, 兼任講師 (50555109)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: チタン / インプラント / 酸化ストレス / 細胞 / 陽極酸化処理 / 石灰化組織 / 創傷治癒

Outline of Final Research Achievements

Cells adhering onto implant surfaces are subjected to oxidative stress during wound healing processes. Although titanium and its alloys are among the most frequently used biomaterials in orthopedic and dental implants, titanium surfaces do not have antioxidant properties, and cells grown on these surfaces can show permanent oxidative stress. The present study assessed the antioxidant property and osteogenic properties of titanium samples with or without oxidation treatments. A thick rutile TiO2 film was observed on thermally oxidized titanium surfaces, while amorphous anatase TiO2 formed on anodically oxidized titanium surfaces prepared by discharging in 1 M Na2HPO4. A resistance to the depletion of reduced glutathione in adherent osteoblasts, which correlates with antioxidant behavior, occurred on anodically oxidized titanium. Enhanced osteogenic gene expressions and nano-biomechanical properties of mineralized tissue were achieved on anodically oxidized titanium.

Research Products

• [Journal Article] A review of improved fixation methods for dental implants. Part I: Surface optimization for rapid osseointegration (2015)
  • Author(s): Shibata Y., Tanimoto Y.
  • Journal Title: Journal of Prosthodontic Research Volume: 59 Issue: 1 Pages: 20-33
  • DOI: 10.1016/j.jpor.2014.11.007
  • NAID: 130007936698
  • ISSN: 1883-1958, 1883-9207
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] A review of improved fixation methods for dental implants. Part II: Biomechanical integrity at bone–implant interface (2015)
  • Author(s): Shibata Y., Tanimoto Y., Maruyama N., Nagakura M.
  • Journal Title: Journal of Prosthodontic Research Volume: 59 Issue: 2 Pages: 84-95
  • DOI: 10.1016/j.jpor.2015.01.003
  • NAID: 130007936685
  • ISSN: 1883-1958, 1883-9207
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Surface topography, hardness, and frictional properties of GFRP for esthetic orthodontic wires (2015)
  • Author(s): Inami T., Tanimoto Y., Yamaguchi M., Shibata Y., Nishiyama N., Kasai K.
  • Journal Title: Journal of Biomedical Materials Research Part B: Applied Biomaterials Volume: 103 Issue: 1 Pages: 88-95
  • DOI: 10.1002/jbm.b.33372
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Enhanced in vitro biological activities generated by surface characteristics of anodically oxidized titanium- the contribution of the oxidation effect (2015)
  • Author(s): Wurihan, Yamada A., Suzuki D., Shibata Y., Kamijo R, and Miyazaki T.
  • Journal Title: European Cells and Materials Volume: ２９
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Bone micro-fragility caused by the mimetic aging processes in α-klotho deficient mice: in situ nanoindentation assessment of dilatational bands (2015)
  • Author(s): Maruyama N., Shibata Y., Mochizuki A., Yamada A., Maki K., Inoue T., Kamijo R., Miyazaki T.
  • Journal Title: Ｂｉｏｍａｔｅｒｉａｌｓ Volume: ４７ Pages: 62-71
  • DOI: 10.1016/j.biomaterials.2015.01.004
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] In-situ quasi-static and dynamic nanoindentation tests on calcified nodules formed by osteoblasts: Implication of glucocorticoids responsible for osteoblast calcification (2015)
  • Author(s): Miyamoto S., Miyamoto Y., Shibata Y., Yoshimura K., Izumida E., Suzuki H., Miyazaki T., Maki K., Kamijo R.
  • Journal Title: Acta Biomaterialia Volume: １２ Pages: 216-226
  • DOI: 10.1016/j.actbio.2014.10.038
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Strain-rate stiffening of cortical bone: observations and implications from nanoindentation experiments (2014)
  • Author(s): Maruyama N., Shibata Y., Wurihan, Swain M., Kataoka Y., Takiguchi Y., Yamada A., Maki K. and Miyazaki T.
  • Journal Title: Ｎａｎｏｓｃａｌｅ Volume: ６ Issue: 24 Pages: 14863-14871
  • DOI: 10.1039/c4nr03180f
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Nanoindentation tests to assess polymerization of resin-based luting cement (2014)
  • Author(s): Sato M., Fujishima A., Shibata Y., Miyazaki T.
  • Journal Title: Ｄｅｎｔａｌ Ｍａｔｅｒｉａｌｓ Volume: ３０ Issue: 9 Pages: 1021-1028
  • DOI: 10.1016/j.dental.2014.05.034
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Nanomechanical properties and molecular structures of in vitro mineralized tissues on anodically oxidized titanium surfaces. (2014)
  • Author(s): Jang, I-. K., Tanaka, R., Wurihan, Suzuki, D., Shibata Y., Fujisawa, N., Tanimoto, Y., Ogura, K., Kamijo, R., Miyazaki, T.
  • Journal Title: Nanomedicine Volume: 10 Issue: 3 Pages: 629-637
  • DOI: 10.1016/j.nano.2013.09.007
  • Peer Reviewed
• [Journal Article] Lysyl oxidase like-2 reinforces unsatisfactory ossification induced by bone morphogenetic protein-2: relating nanomechanical properties and molecular changes. (2013)
  • Author(s): Shibata, Y., Suzuki, D., Wurihan, Yamada, A., Maruyama, N., Fujisawa, N., Kamijo, R., Miyazaki, T.
  • Journal Title: Nanomedicine Volume: 9 Issue: 7 Pages: 1036-1047
  • DOI: 10.1016/j.nano.2013.04.008
  • Peer Reviewed
• [Journal Article] Morphology and In Vitro Behavior of Electrospun Fibrous Poly(D,L-lactic acid) for Biomedical Applications. (2013)
  • Author(s): Inami T., Tanimoto Y., Ueda M., Shibata Y., Hirayama S., Yamaguchi M., and Kasai K.
  • Journal Title: Advances in Materials Science and Engineering Volume: 140643 Pages: 1-6
  • DOI: 10.1155/2013/140643
  • Peer Reviewed
• [Journal Article] In vitro demineralization of tooth enamel subjected to two whitening regimes. (2013)
  • Author(s): Ogura K., Tanaka R., Shibata Y., Miyazaki T., Hisamitsu H.
  • Journal Title: Journal of the American Dental Association Volume: 144 Issue: 7 Pages: 799-807
  • DOI: 10.14219/jada.archive.2013.0190
  • Peer Reviewed
• [Journal Article] A new modified laser pretreatment for porcelain zirconia bonding (2013)
  • Author(s): Liu Dan
  • Journal Title: Dental Materials Volume: 29 Issue: 5 Pages: 559-565
  • DOI: 10.1016/j.dental.2013.03.002
  • Peer Reviewed
• [Journal Article] Lysyl oxidase like-2 reinforces unsatisfactory ossification induced by bone morphogenetic protein-2: relating nanomechanical properties and molecular changes. (2013)
  • Author(s): Shibata Y.
  • Journal Title: Nanomedicine: Nanotechnology, Biology, Medicine Volume: 9
  • Peer Reviewed
• [Journal Article] In vitro demineralization of tooth enamel subjected to two whitening regimes. (2013)
  • Author(s): Ogura K.
  • Journal Title: Journal of the American Dental Association Volume: 144
  • Peer Reviewed
• [Journal Article] Antioxidant and osteogenic properties of anodically oxidized titanium (2012)
  • Author(s): Iwai-Yoshida M., Shibata Y., Wurihan, Suzuki D., Fujisawa N., Tanimoto Y., Kamijo R., Maki K. and Miyazaki T.
  • Journal Title: Journal of the Mechanical Behavior of Biomedical Materials Volume: (in press) Pages: 230-236
  • DOI: 10.1016/j.jmbbm.2012.01.016
  • Peer Reviewed
• [Journal Article] Tape-cast and sintered beta-tricalcium phosphate laminates for biomedical applications: Effect of milled A1203 fiber additives on microstructural and (2012)
  • Author(s): Tanimoto Y,
  • Journal Title: Journal of Biomedical Materials Research Part B: Applied Biomaterials Volume: 100 Issue: 8 Pages: 2261-2268
  • DOI: 10.1002/jbm.b.32796
  • Peer Reviewed
• [Presentation] Age-related degradation of mouse cortical bone:implications for alfa-klotho gene responsible for bone mechanical integrity in a series of nanoindentation experiments (2014)
  • Author(s): Maruyama N., Shibata Y., Mochizuki A., Miyazaki T., Inoue T., Maki K.
  • Organizer: APCFS/SIF-2014
  • Place of Presentation: オーストラリア シドニー
  • Year and Date: 2014-12-09 – 2014-12-12
• [Presentation] Nanoindentation tests to assess polymerization quality of resin-based luting cement (2014)
  • Author(s): 柴田 陽
  • Organizer: The 3rd trilateral dental symposium on advance in zirconia and its use as a dental materials
  • Place of Presentation: 東京
  • Year and Date: 2014-06-15
  • Invited
• [Presentation] Nanomechanical Testing Technology for In Vitro Mineralized Tissue. (2013)
  • Author(s): Shibata Y., Suzuki D., Wurihan., Yamada A., Maruyama N., Kamijo R., Miyazaki T.
  • Organizer: 2nd Meeting of the International Association for Dental Research- Asia Pacific Region
  • Place of Presentation: タイ バンコク
• [Presentation] Nanomechanical testing technology for engineered tissues (2013)
  • Author(s): Yo Shibata
  • Organizer: THE 2ndTRILATERAL DENTAL SYMPOSIUM on “DENTAL VIRTUAL EDUCATION TODAY”
  • Place of Presentation: 日本歯科大学
  • Invited
• [Presentation] Nano mechanical testing technology for in vitro mineralized tissue on titanium (2012)
  • Author(s): Wurihan
  • Organizer: The 1st Trilateral “Zirconia & Titanium Today” Dental Materials Science Symposium
  • Place of Presentation: 香港
  • Invited
• [Presentation] Contact osteogenesis of titanium implants stimulated by wire-type electric discharge machining and an irradiation of ultraviolet lamp (2012)
  • Author(s): Kataoka Y.
  • Organizer: The 1st Trilateral “Zirconia & Titanium Today” Dental Materials Science Symposium
  • Place of Presentation: 香港
  • Invited
• [Book] The New Frontiers in Research for Oral Cancer (2013)
  • Author(s): Yo Shibata, Takashi Miyazaki
  • Total Pages: 209
  • Publisher: MARUZEN PLANET