The isolation and identification of the higher bone differentiation of mesenchymal stem cell from the pulp of human teeth and the application for the jaw bone regeneration.

• Project/Area Number: 24592980
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: University of Tsukuba
• Principal Investigator: YAMAGATA Kenji 筑波大学, 医学医療系, 講師 (00420084)
• Co-Investigator(Kenkyū-buntansha): OHNEDA Osamu 筑波大学, 医学医療系, 教授 (30311872) ; NAGANO Masumi 筑波大学, 医学医療系, 講師 (30436282)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 間葉系幹細胞 / 細胞表面マーカー / 自家移植 / 骨分化能 / Runx2 / ALP / TGF-beta

Outline of Final Research Achievements

In the crown completion stage of the dental pulp derived mesenchymal stem cells (DPSCs), the expression of the Runx2 have been increased significantly in the DPSCs of root formation stage and root completed stage from the previous time of bone differentiation induction. This result was considered due to the Runx2 stage abnormal specific expression for potential the suppression of bone differentiation. In the bone differentiation process of crown completed stage from DPSCs, expression of ALP were significantly lower form the other timing derived DPSCs. Therefore, the expression of Runx2 was analyzed for bone differentiation potential by the gene transfer of ALP to the DPSCs of crown completion. As a result, the normal bone differentiation induction was observed in the ALP gene transfer group. But it revealed that suppressing the expression of Runx2 is not observed. In the future, we will analyze a system for regulation of expression of Runx2 in DPSC.

Research Products

• [Journal Article] The role of CCL5 in the ability of adipose tissue-derived mesenchymal stem cells to support repair of ischemic regions. (2014)
  • Author(s): Kimura K, Nagano M, Salazar G, Yamashita T, Tsuboi I, Mishima H, Matsushita S, Sato F, Yamagata K, Ohneda O.
  • Journal Title: Stem Cells Dev. Volume: 23(5) Issue: 5 Pages: 488-501
  • DOI: 10.1089/scd.2013.0307
  • Peer Reviewed
• [Journal Article] Interleukin-4 receptor α-based hybrid peptide effectively induces antitumor activity in head and neck squamous cell carcinoma. (2013)
  • Author(s): Seto K, Shoda J, Horibe T, Warabi E, Ishige K, Yamagata K, Kohno M, Yanagawa T, Bukawa H, Kawakami K.
  • Journal Title: Oncol Rep. Volume: 29(6) Pages: 2147-53
  • Peer Reviewed
• [Journal Article] Amelioration of cisplatin-induced nephrotoxicity in peroxiredoxin I-deficient mice. (2013)
  • Author(s): Okada K, Ma D, Warabi E, Morito N, Akiyama K, Murata Y, Yamagata K, Bukawa H, Shoda J, Ishii T, Yanagawa T.
  • Journal Title: Cancer Chemother Pharmacol. Volume: 71(2) Pages: 503-9
  • Peer Reviewed