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Investigation on phenotypes of macrophages in transplantation of engineered tissues and its application to regenerative medicine

Research Project

Project/Area Number 24592981
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Surgical dentistry
Research InstitutionThe University of Tokyo

Principal Investigator

YUKO FUJIHARA  東京大学, 医学(系)研究科(研究院), 特任助教 (50466744)

Co-Investigator(Kenkyū-buntansha) HOSHI Kazuto  東京大学, 医学部附属病院, 准教授 (30344451)
MORI Yoshiyuki  自治医科大学, 医学部, 教授 (70251296)
Co-Investigator(Renkei-kenkyūsha) TAKATO Tsuyoshi  東京大学, 医学部附属病院, 教授 (90171454)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords再生医療 / 軟骨再生 / 組織反応 / 軟骨再生医療
Outline of Final Research Achievements

In this study, we investigated the properties of macrophages in transplantation of tissue-engineered cartilage. As to methods, human auricular chondrocytes were cultured and embedded in polylactic acid scaffold, and then transplanted subcutaneously in BALB/c-nu/nu mice. Immunohitochemical localization of F4/80-positive cells was increased by 2 weeks. Meanwhile, M1-prone macrophages were dominant in the early-stage of transplantation up to around 2 weeks, and then M2-prone macrophages surpassed them after that. Furthermore, suppressing macrophages with clodronate liposome immediate after transplantation increased the accumulation of sGAG at eight weeks. It was therefore suggested that suppression of early-stage macrophages, which were considered to be M1-prone, could be advantageous for cartilage regeneration.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (7 results)

All 2014 2013

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 1 results) Presentation (5 results)

  • [Journal Article] Preclinical and clinical research on bone and cartilage regenerative medicine in oral and maxillofacial region2014

    • Author(s)
      Takato T, Mori Y, Fujihara Y, Asawa Y, Nishizawa S, Kanazawa S, Ogasawara T, Saijo T, Abe T, Abe M, Suenaga H, Kanno Y, Sugiyama S, Hoshi K
    • Journal Title

      Oral Sci Int

      Volume: 11 Pages: 45-51

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Macrophage-inducing fasl on chondrocytes forms immune privilege in cartilage tissue engineering, enhancing in vivo regeneration2014

    • Author(s)
      Fujihara Y, Takato T, Hoshi K, Fujihara Y, Takato T, Hoshi K
    • Journal Title

      Stem Cells

      Volume: 印刷中 Issue: 5 Pages: 1208-19

    • DOI

      10.1002/stem.1636

    • Related Report
      2014 Annual Research Report 2013 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] マクロファージのサブクラスが軟骨再生に与える影響2014

    • Author(s)
      藤原夕子,高戸毅,星和人
    • Organizer
      第35回日本炎症・再生医学会
    • Place of Presentation
      万国津梁館,沖縄
    • Year and Date
      2014-07-01 – 2014-07-04
    • Related Report
      2014 Annual Research Report
  • [Presentation] 再生軟骨移植における組織反応抑制メカニズムの解析とその臨床応用2013

    • Author(s)
      藤原夕子、星和人、高戸毅
    • Organizer
      第67回日本口腔科学会学術集会
    • Place of Presentation
      栃木県総合文化センター
    • Related Report
      2013 Research-status Report
  • [Presentation] Macrophage-inducing FasL on chondrocytes forms immune privilege in cartilage tissue engineering, enhancing in vivo regeneration2013

    • Author(s)
      Fujihara Y, Takato T, Hoshi K
    • Organizer
      Frontiers 2013 EPFL Symposium
    • Place of Presentation
      École Polytechnique Fédérale de Lausanne, EPF Lausanne, EPFL, Lausanne, Switzerland
    • Related Report
      2013 Research-status Report
  • [Presentation] Phenotypes of macrophages in transplantation of tissue-engineered cartilage.2013

    • Author(s)
      Fujihara Y, Hoshi K.
    • Organizer
      第42回日本免疫学会総会
    • Place of Presentation
      幕張メッセ
    • Related Report
      2013 Research-status Report
  • [Presentation] 再生軟骨移植におけるマクロファージサブクラスの検討2013

    • Author(s)
      藤原夕子、高戸毅、星和人
    • Organizer
      第12回日本再生医療学会総会
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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