Novel therapeutic approach using beta-glucan based oligonucleotides delivery system for osteoporosis
| Project/Area Number |
24593000
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Kyushu Dental College |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 破骨細胞 / シゾフィラン / dectin-1 / ドラッグデリバリー / カードラン |
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| Outline of Final Research Achievements |
Several immune system cell surface receptors are reported to be associated with osteoclastogenesis. Dectin 1, a lectin receptor for β-glucan, is found predominantly on cells of the myeloid lineage. In this study we have developed a delivery system for oligonucleotides using schizophyllan (SPG), a polysaccharide that belongs to the β-glucan family. Application of complex consisting SPG and TNF receptor associated factor 6 (TRAF6) specific siRNA to osteoclast progenitor cells inhibited the osteoclast formation in vitro.
On the other hand, we also demonstrated that the dectin-1 agonist curdlan potentially inhibits osteoclast differentiation, especially nuclear factor of activated T cell cytoplasmic 1 (NFATc1) expression, and that Syk kinase plays a crucial role in the transcriptional pathways.
These data suggest that application of this new complex and curdlan administration could be potential candidates for the treatment of osteoclast-related diseases such as osteoporosis.
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Report
(4 results)
Research Products
(72 results)
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[Journal Article] Mechanisms of G1 cell cycle arrest and apoptosis in myeloma cells induced by hybrid-compound histone deacetylase inhibitor2013
Author(s)
Fujii, S., Okinaga, T., Ariyoshi, W., Takahashi, O., Iwanaga, K., Nishino, N., Tominaga, K., Nishihara, T
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Journal Title
Biochem Biophys Res Commun
Volume: 434
Issue: 3
Pages: 413-20
DOI
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Peer Reviewed
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[Presentation] Mechanical stress suppresses the osteoclastgenesis in RAW264.7 cells through IL-33 secretion in MC3T3-E1 cells2015
Author(s)
HIROYASU KIYOMIYA, WATARU ARIYOSHI, TOSHINORI OKINAGA, TAKESHI KANEUJI, SHO MITSUGI, TAKUMA SAKURAI, MANABU HABU, IZUMI YOSHIOKA, KAZUHIRO TOMINAGA, TATSUJI NISHIHARA
Organizer
Asia-Pacific Conference in Fukuoka 2015- International Symposium on Oral Education and Research in Kitakyushu
Place of Presentation
Kitakyushu, Japan
Year and Date
2015-01-25
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[Presentation] Classification of periodontal disease patients by FT-IR
Author(s)
SATOSHI FUJII, KEISUKE FUKUDA、SHINOBU SATO, TOSHINORI OKINAGA, WATARU ARIYOSHI, KEISUKE NAKASHIMA, TATSUJI NISHIHARA, SHIGEORI TAKENAKA
Organizer
Asia-Pacific Conference in Fukuoka 2013- International Symposium on Oral Education and Research in Kitakyushu
Place of Presentation
Kitakyushu, Japan
Related Report
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