| Project/Area Number |
24593001
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Health Sciences University of Hokkaido |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
MURATA Masaru 北海道医療大学, 歯学部, 准教授 (00260662)
HAMADA Junichi 北海道医療大学, 看護福祉学部, 教授 (50192703)
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| Project Period (FY) |
2012-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 歯髄幹細胞 / HOX / 分化誘導 / 歯髄由来幹細胞 / ヒト歯髄由来幹細胞 / HOX遺伝子群 |
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| Outline of Final Research Achievements |
In this study, we examined expression pattern of HOX genes in the stem cell that collected from human dental pulp. As for the stem cell provided by the neurosphere method, expression of HOXC group increased. As for this result, It is assumed that a neural progenitor cells was included in in this stem cell was thought about. On the other hand, HOXA, expression of HOXB group rose in Dental Pulp Stem Cells(DPSC/AllCells).This cell resembled a mesenchymal stem cell and was the result that was different from a provided cell in the neurosphere method. It was found that it was difficult to establish a totipotent stem cell than human dental pulp, because a result varied according to a method of the purification of the stem cell derived from human dental pulp. We introduced HOXC 5 genes expression plasmid vector into DPSC and examined the differentiation to nerve cells, but the differentiation was not confirmed.
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