Establishment of a new treatment of oral stomatitis associated with cancer chemotherapy using a phytochemical

• Project/Area Number: 24593013
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Kanagawa Dental College
• Principal Investigator: YOSHINO FUMIHKO 神奈川歯科大学, 歯学研究科(研究院), 准教授 (20308307)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 口内炎 / ファイトケミカル / 抗酸化作用 / 化学療法 / 脂質過酸化 / 酸化ストレス / 活性酸素種 / 抗癌剤 / 活性酸素 / 抗酸化剤

Outline of Final Research Achievements

The purpose of this study was thus to demonstrate the involvement of reactive oxygen species (ROS) and the alteration of the redox state in oral mucositis using an electron spin resonance (ESR). An oral mucositis animal model induced by treatment of 5-fluorouracil with 10% acetic acid in hamster cheek pouch. Lipid peroxidation was measured and the rate constants of the signal decay of nitroxyl compounds using in vivo L-band ESR were calculated from the signal decay curves. We established the oral mucositis animal model induced in hamster cheek pouch. An increased level of lipid peroxidation was found by measuring malondialdehyde. The decay rate constants of carbamoyl-PROXYL were decreased. These disorders was inhibited by treatment of alpha-glucosyl-hesperidine. These results suggest that a redox imbalance might occur by excessive generation of ROS at an early stage of oral mucositis and the consumption of large quantities of antioxidants in the locality of oral mucositis.

Research Products

• [Journal Article] Alteration of the redoxstate with reactive oxygen species for 5-fluorouracil- induced oral mucositis in hamsters (2013)
  • Author(s): Yoshino F, Yoshida A, Nakajima A, Wada-Takahashi S, Takahashi S-S, Lee M-C
  • Journal Title: PLoS ONE Volume: 8(12) Issue: 12 Pages: 1-6
  • DOI: 10.1371/journal.pone.0082834
  • Peer Reviewed
• [Presentation] ハムスターを用いた癌化学療法に併発する口内炎における活性酸素評価 (2014)
  • Author(s): 吉野文彦，吉田彩佳，高橋俊介，高橋聡子，前畑洋次郎，宮本千央，李 昌一
  • Organizer: 第14回 日本抗加齢医学会総会
  • Place of Presentation: 吹田市
  • Year and Date: 2014-06-06 – 2014-06-08
• [Presentation] 化学療法において併発する口内炎における活性酸素評価 (2013)
  • Author(s): 吉野文彦，吉田彩佳，高橋俊介，高橋聡子，前畑洋次郎，宮本千央，李 昌一
  • Organizer: 神奈川歯科大学学会 第 48 回総会
  • Place of Presentation: 横須賀
• [Remarks] 神奈川歯科大学大学院歯学研究科口腔科学講座光歯科医学分野ホームページ
  • URL: http://www.labs.kdu.ac.jp/pmd/