| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Outline of Final Research Achievements |
To evaluate the association of poly (ADP-ribose) metabolism on reprogramming, we examined gene or miRNA expressions comprehensively under the deficiency of Parp1 and Parg. From our earlier study, we ascertained that the expression of reprogramming markers increased after conversion to different lineage cells in dental pulp cells, suggesting that those levels are key events on the reprogramming processes. It is particularly interesting that the level of reprogramming markers was lower in Parp1-deficient embryonic stem cells than in wild type cells. Moreover, differential expression of miRNA associated with reprogramming factors was detected in Parg-deficient embryonic stem cells. Consequently, this study investigated mechanisms for reprogramming factors associated with poly (ADP-ribose) metabolism.
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