| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Outline of Final Research Achievements |
It is often difficult to treat advanced, recurrent or unresectable oral cancers. A new strategy must be needed. In these years, the cancer stem cells (CSCs) are an important target for the development of novel strategies in above oral cancer treatment. However, CSCs-targeted new anti-cancer drug discovery is currently hindered by the lack of easy and reliable methods for collecting sufficient number of CSCs. Briefly,artificially-manufactured cancer stem cells are essential. In this project,we could generate the transfectant cells ( HSC2/hOCT3/4-shp53-F+hSK+hUL) with CSCs properties by introduction of defined reprogramming factors (Oct3/4, sh53, Sox2, Klf4, l-Myc and Lin28) into HSC2 tongue cancer cells. Our findings suggest that artificial cancer stem cells developed by cellular reprogramming may be useful for investigating the acquisition of potential malignancy as well as screening the CSCs-targeting drugs.
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