The study of the site of action of volatile general anesthetics using ESR and NMR
| Project/Area Number |
24593044
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Hokkaido University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
平沖 敏文 北海道大学, 工学(系)研究科(研究院), 准教授 (10125346)
長谷 由理 北海道大学, 大学病院, 助教 (20626121)
藤澤 俊明 北海道大学, 歯学研究科, 教授 (30190028)
鈴木 邦明 北海道大学, 歯学研究科(研究院), 教授 (40133748)
木村 幸文 北海道大学, 大学病院, 講師 (00292037)
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| Research Collaborator |
HONMA Shouichi
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| Project Period (FY) |
2012-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 全身麻酔薬 / 麻酔薬の作用機序 / 電子スピン共鳴 / 核磁気共鳴 / スピンラベル剤 / 生体モデル膜 / リポソーム / スピンラベル / 化学交換 |
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| Outline of Final Research Achievements |
In the research using the electron spin resonance method, the following four results are shown. ①Desflurane remains on the surface of lipid bilayer in liposome. ②The existence of Na,K-ATPase does not affect the environment around the spin labels in the liposome and the effects of anesthetics on liposome as a model membrane. ③The fluidity of liposome membrane increase with rises in the temperature and the existence of anesthetics. ④The difference in membrane form does not affect the action of anesthetics on liposome.
In the study using nuclear magnetic resonance method, the following two results are shown. ①Anesthetic does chemical-exchange process between free and bound anesthetic on liposome. ②Isoflurane molecules locate in the outer surface of liposome. This result supports our research results using ESR.
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Report
(6 results)
Research Products
(2 results)
