| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
In periosteum supposed that there were abundant multipotent stem cells to date.
To identify the characterization of periosteum stem cell and realize the isolation system of them, we performed the assay of cell colony formation and proliferation using stem cells derived from human periosteum for bone regenerative medicine substantively. In the human identical cell group, the different population of cell division rate displayed d by Flowcytometer. The rapid division group showed the ability of agrregate-colony formation, producing the extracellular matrix and maintaining the pluripotential as compared with a late division group. To evaluate the property of rapid division group were transplanted into defect of canine mandibular bone. As results, the bone matrix production was observed by histological staining, soft X-ray findings, microCT analysis for two months.
This study identified the optimal condition of pluripotential stem cells derived from periosteum.
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