Involvement of the Warburg effect in the regulation of ccn2 gene expression

• Project/Area Number: 24593060
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Showa University
• Principal Investigator: KONDO Seiji 昭和大学, 歯学部, 准教授 (10432634)
• Co-Investigator(Kenkyū-buntansha): KAMATANI Takaaki 昭和大学, 歯学部, 助教 (00315003) ; MUKUDAI Yoshiki 昭和大学, 歯学部, 助教 (50325099) ; KURIHARA Yuji 昭和大学, 歯学部, 助教 (90514969) ; 新谷 悟 昭和大学, 歯学部, 教授 (80294429)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 低酸素

Outline of Final Research Achievements

A novel GAPDH-mediated gene regulatory mechanism controlling the stability of ccn2 mRNA during hypoxia is uncovered. The precise regulatory mechanism of the degradation of ccn2 mRNA by the interaction among the ccn2 3'-UTR and GAPDH was identified. The observed redox regulation would supposedly be important for switching ccn2 gene expression upon the exposure to hypoxic or oxidative stress.

Research Products

• [Journal Article] Computer-assisted surgery for mandibular reconstruction using a patient-specific titanium mesh tray and particulate cancellous bone and marrow (2015)
  • Author(s): Seiji Kondo, Hideyuki Katsuta, Ayako Akizuki, Yuji Kurihara, Takaaki Kamatani, Atsushi Yaso, Masahiro Nagasaki, Toshikazu Shimane, Tatsuo Shirota
  • Journal Title: Case Reports in Clinical Medicine Volume: 4 Pages: 85-92
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Defferential expression of vascular endothelial growth factor in high- and low-metastasis cell lines of salivary gland adenoid cystic carcinoma (2014)
  • Author(s): Seiji Kondo, Yoshiki Mukudai, Daisuke Soga, Takashi Nishida, Sato D, Masaharu Takigawa, Tatsuo Shirota
  • Journal Title: Anticancer research Volume: 34 Pages: 671-678
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Potential anti-osteoporotic effects of herbal extracts on osteoclasts, osteoblasts and chondrocytes in vitro. (2014)
  • Author(s): *Mukudai, Y, Kondo, S., Koyama, T., Li C., Banka, S., Kogure, A., Yazawa, K., Shintani, S.
  • Journal Title: BMC Complement Altern Med. Volume: 14 Issue: 1 Pages: 29-29
  • DOI: 10.1186/1472-6882-14-29
  • Peer Reviewed / Open Access
• [Journal Article] A potential anticancer activity of extracts derived from the roots of Scutellaria baicalensis on humanoral squamous cell carcinoma cells (2013)
  • Author(s): Sato D, Yazawa K, Kondo S, Mukudai Y, Li C,Kamatani T, Katsuta H, Yoshihama Y,Shirota T,Shintani S
  • Journal Title: Molecular and Clinical Oncology Volume: 1 Issue: 1 Pages: 105-111
  • DOI: 10.3892/mco.2012.14
  • Peer Reviewed
• [Journal Article] Antitumor effects of telomerase-specific replication-selective oncolytic viruses for adenoid cystic carcinoma cell lines (2013)
  • Author(s): Daisuke Sato, Yuji Kurihara, Seiji Kondo, Tatsuo Shirota, Yasuo Urata, Toshiyoshi Fujiwara, Satoru Shintani
  • Journal Title: Oncology Reports Volume: 30 Issue: 6 Pages: 2659-2664
  • DOI: 10.3892/or.2013.2738
  • Peer Reviewed
• [Journal Article] Root bark extracts of Juncus effusus and Paeonia suffruticosa protect salivary gland acinar cells from apoptotic cell death induced by cis-platinum (II) diammine dichloride (2013)
  • Author(s): Yoshiki Mukudai, Seiji Kondo, Sunao Shiogama, Tomoyuki Koyama, Chunnan Li, Kazunaga Yazawa, Satoru Shintani
  • Journal Title: Oncology Reports Volume: 30 Issue: 6 Pages: 2665-2671
  • DOI: 10.3892/or.2013.2744
  • Peer Reviewed
• [Journal Article] microRNA expression profiles in oral squamous cell carcinoma (2013)
  • Author(s): Daisuke Soga, Sayaka Yoshiba, Sunao Shiogama, Hiroaki Miyazaki, Seiji Kondo, Satoru Shintani
  • Journal Title: Oncology Reports Volume: 30 Issue: 2 Pages: 579-583
  • DOI: 10.3892/or.2013.2488
  • Peer Reviewed
• [Journal Article] The root bark of Paeonia moutan is a potential anticancer agent in human oral squamous cell carcinoma cells (2012)
  • Author(s): Li C, Yazawa K, Kondo S, Mukudai Y, Sato D, Kurihara Y, Kamatani T, Shintani S
  • Journal Title: Anticancer research Volume: 32 Pages: 2625-2630
  • Peer Reviewed
• [Journal Article] A combination of chemical and mechanical stimuli enhances not only osteo-but also chondro-differentiation in adipose-derived cells (2012)
  • Author(s): Banka S, Mukudai Y, Yoshihama Y, Shirota T, Kondo S, Shintani S
  • Journal Title: Journal of oral Biosciences Volume: 54 Pages: 188-195
  • Peer Reviewed
• [Journal Article] Role of low-density lipoprotein receptor related protein 1 (LRP1) in CCN2/connective tissue growth factor (CTGF) protein transport in chondrocytes. (2012)
  • Author(s): Kawata., K., Kubota, S., Eguchi, T., Aoyama, E., Moritani, N., Kondo, S., Nishida, T., Takigawa, M.
  • Journal Title: J Cell Sci Volume: 15 Pages: 2965-2972
  • DOI: 10.1242/jcs.101956
  • Peer Reviewed
• [Presentation] Differential expression of vascular endothelial growth factor in high and low metastasis cell lines of Ssalivary gland adenoid cystic carcinoma (2014)
  • Author(s): Seiji Kondo, Yoshiki Mukudai, Daisuke Soga, Tatsuo Shirota
  • Organizer: 11th asian congress on oral and maxillofacial surgery. 11th asian congress on oral and maxillofacial surgery
  • Place of Presentation: Xi’an, Chaina
  • Year and Date: 2014-08-22 – 2014-08-25
• [Presentation] 高転移性、低転移性腺様嚢胞癌細胞株におけるVEGF発現の差異 (2013)
  • Author(s): 近藤誠二 椋代義樹 曽我大輔 新谷 悟
  • Organizer: 第37回日本頭頸部癌学会
  • Place of Presentation: 東京
• [Presentation] Telomerase-Specific Replication-Selective Virotherapy combined with radiation therapy for oral squamous cell carcinoma cells (2012)
  • Author(s): Yuji Kurihara, Daisuke Sato, Tatsuo Shirota, Seiji Kondo, Masafumi Kataoka, Yasuo Urata, Toshiyoshi Fujiwara, Satoru Shintani
  • Organizer: 2012AACR
  • Place of Presentation: chicago, Illinois, USA
• [Presentation] Telomerase-Specific Replication-Selective oncolytic viruses for adenoid cystic (2012)
  • Author(s): Daisuke Sato, Yuji Kurihara, Seiji Kondo, Tatsuo Shirota, Yasuo Urata, Toshiyoshi Fujiwara, Satoru Shintani
  • Organizer: 2012AACR
  • Place of Presentation: chicago, Illinois, US
• [Presentation] Binding of glyceraldehyde-3-phosphate dehydrogenase to the cis-acting element of structure-anchored repression in ccn2 mRNA (2012)
  • Author(s): Seiji Kondo,Satoshi Kubota, Yasuto Yoshihama, Satoru Shintani, Masaharu Takigawa
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: 札幌