| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
The study plan is to clarify the mechanism of growth and invasion of oral cancer. Proteome analysis was perofmed for a human normal mucosal epithelium, epithelial dysplasia, CIS and invasive cancer, were excised epithelium from each tissue. The factor expressig in a progression to the cancer, were keratin, HSP, Lamin A / C, desmoplakin, and plectin-1, was it's elevated in a protein level. Then in order to verify the change of the cells with the progression to invasive cancer, we have devloped 3D cancer invasion model that using oral SCC lines and human myoma tissue. In addition, we also develop the SLPI gene knock down Ca9 cell lines, named NUSD-1, which revealed invasiveness to the myoma. Immunohistochemistry and gene expression analysis demostrated a infiltration kinetics by 3-dimensional culture. As a result, responsive factors involve in cancer invasion of this model, which are MMP2, MMP9 and TIMP. These expression patterns of factors, revealed to control a tumor invasion.
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