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New 3-dimentional culture model of early invasion of oral cancer based on proteome analysis.

Research Project

Project/Area Number 24593066
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Surgical dentistry
Research InstitutionNihon University

Principal Investigator

KOMIYAMA Kazuo  日本大学, 歯学部, 教授 (00120452)

Co-Investigator(Kenkyū-buntansha) MATSUMOTO Naoyuki  日本大学, 歯学部, 助教 (20386080)
Research Collaborator IWASE Takashi  
ASANO Masatake  
OOKI Hidero  
OMAGARI Daisuke  
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords口腔癌 / 初期浸潤 / proteome / 3D culture / SLPI / NUSD-1 / MMP / TIMP / ptoteome / 3次元培養 / 浸潤能 / myoma disk / SLIPI / cytokeratin / plectin / 癌浸潤 / lamin / plectin-1 / desmoplakin / HSP / keratin
Outline of Final Research Achievements

The study plan is to clarify the mechanism of growth and invasion of oral cancer. Proteome analysis was perofmed for a human normal mucosal epithelium, epithelial dysplasia, CIS and invasive cancer, were excised epithelium from each tissue. The factor expressig in a progression to the cancer, were keratin, HSP, Lamin A / C, desmoplakin, and plectin-1, was it's elevated in a protein level. Then in order to verify the change of the cells with the progression to invasive cancer, we have devloped 3D cancer invasion model that using oral SCC lines and human myoma tissue. In addition, we also develop the SLPI gene knock down Ca9 cell lines, named NUSD-1, which revealed invasiveness to the myoma. Immunohistochemistry and gene expression analysis demostrated a infiltration kinetics by 3-dimensional culture. As a result, responsive factors involve in cancer invasion of this model, which are MMP2, MMP9 and TIMP. These expression patterns of factors, revealed to control a tumor invasion.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (4 results)

All 2014

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (3 results) (of which Invited: 1 results)

  • [Journal Article] Sodium Butyrate, a Histone Deacetylase Inhibitor, Regulates Lymphangiogenic Factors in Oral Cancer Cell Line HSC-32014

    • Author(s)
      TAKASHI YAMAMURA, NAOYUKI MATSUMOTO,YASUYOSHI MATSUE , MICHISATO OKUDERA,YOUICHI NISHIKAWA, YOSHIMITSU ABIKO,KAZUO KOMIYAMA
    • Journal Title

      Anticancer Research

      Volume: 34 Pages: 1701-1708

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] 口腔粘膜アレルギー up to date.2014

    • Author(s)
      小宮山一雄
    • Organizer
      第 63 回日本歯科理工学会春期学術講演会
    • Place of Presentation
      タワーホール船堀(東京都江戸川区)
    • Year and Date
      2014-04-12 – 2014-04-13
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 新規口腔癌浸潤モデルとTGF-bの関与2014

    • Author(s)
      野本翔太,野口博康,岩瀬孝志,田中孝佳,外木守雄,小宮山一雄,大木秀郎
    • Organizer
      第68回日本口腔科学会学術集会
    • Place of Presentation
      京王プラザホテル,東京
    • Related Report
      2013 Research-status Report
  • [Presentation] TAMs polarization and MMPs expression level are relate to metastasis of oral squamous cell carcinom.2014

    • Author(s)
      Kazuo Komiyama, Michisato Okudera, Daisuke Omagari, Yoichi Nishikawa, Masatake Asano
    • Organizer
      17th International Congress on Oral Pathology and Medicine
    • Place of Presentation
      Istanbul, Turkey
    • Related Report
      2013 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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