| Project/Area Number |
24593067
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Azabu University (2014)
The Nippon Dental University (2012-2013) |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
YAGISHITA Hisao 日本歯科大学, 歯学部, 准教授 (50256989)
SATO Kaori 日本歯科大学, 歯学部, 講師 (90287772)
TAYA Yuji 日本歯科大学, 歯学部, 准教授 (30197587)
AOBA Takaaki 日本歯科大学, 歯学部, 教授 (30028807)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 歯学 / 病理学 / 舌癌 / リンパ管新生 / リンパ管浸潤 / 転移 / 病理診断 / 3次元形態計測 / ３次元形態計測 |
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| Outline of Final Research Achievements |
The present study aimed at developing clinicopathological diagnosis of lymphangiogenesis and lymphatic invasion as a prognostic factor in tongue squamous cell carcinoma. During the approved 3-year period, 3D reconstruction was achieved using 100 serial histological sections that were prepared from the invasion front of tongues squamous cell carcinomas. Cancer parenchyma and stroma were characterized by stepwise immunohistochemistry in combination with antibodies (cytokeratin, Ki67, CD31, D2-40, S100A4). 3D inspection in combination of epithelial and mesenchymal markers provided direct evidence for loco-regional invasion patterns, divergent intra- and peritumor microvessel densities, high frequencies of spatial association between carcinoma cells and blood/lymph endothelial cells. The results support the wide potential usage and advantage of histology-based 3D reconstruction in understanding of cancer biology and pathological diagnosis of tumor aggressiveness and malignancy.
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