| Project/Area Number |
24593112
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Orthodontic/Pediatric dentistry
|
|---|
| Research Institution | Matsumoto Dental University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
NAKAMURA Hiroshi 松本歯科大学, 歯学部, 講師 (00278178)
NAKAMICHI Yuko 松本歯科大学, 総合歯科医学研究所, 講師 (20350829)
UDAGAWA Nobuyuki 松本歯科大学, 歯学部, 教授 (70245801)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
ABIKO Yoshimitu 日本大学, 歯学部, 教授 (70050086)
TAGUCHI Akira 松本歯科大学, 歯学部, 教授 (70243582)
SHIMODAIRA Shigetaka 信州大学, 医学部, 教授 (80345751)
|
|---|
| Project Period (FY) |
2012-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Keywords | 歯髄細胞 / 骨髄細胞 / 骨芽細胞 / 石灰化 / アルカリホスファターゼ / 硬組織 / 再生医療 / ALP / BMP-2 / ENPP-1 / 骨髄由来間質細胞 / ヒト / マウス |
|---|
| Outline of Final Research Achievements |
Cultured mouse and human dental pulp (DP) cells exhibited strong alkaline phosphatase (ALP) activity compared with mouse osteoblasts and human mesenchymal stromal cells (MSC). To explore candidate genes to explain the character of DP cells, we conducted a GeneChip microarray analysis to detect differences in expression between DP cells and osteoblasts or MSC. The mRNA level of tissue non-specific ALP and BMP-2 in mouse and human DP cells was strikingly higher than that in mouse osteoblasts or human MSC. In contrast, the mRNA level of ENPP1, which hydrolyzes nucleoside triphosphate (NTP) to inorganic pyrophosphate (PPi), in mouse and human DP cells was much lower than that in mouse osteoblasts or human MSC. The mouse and human DP cells were transplanted into the muscle of immunocompromised NOD/Shi-scid IL2Rg (null) (NOG) mice and then recovered after 2 months. Soft X-ray images showed that explants of DP cells but not control explants formed a dense mineralized mass.
|
