The regulatory mechanism of function on gingival epithelium by irsogladine maleate

• Project/Area Number: 24593123
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Periodontal dentistry
• Research Institution: Hiroshima University
• Principal Investigator: FUJITA TSUYOSHI 広島大学, 大学病院, 講師 (80379883)
• Co-Investigator(Kenkyū-buntansha): MATSUDA Shinji 広島大学, 病院, 病院助教 (30611321)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 歯周病予防 / 歯肉上皮 / アポトーシス / イルソグラジンマレイン酸 / IL-8 / smad2 / ERK / 歯周病原細菌 / 歯肉上皮細胞 / smad2リン酸化 / TGF-betaレセプター / アポトーシスシグナル

Outline of Final Research Achievements

Smad2 was involved in Aggregatibacter actinomycetemcomitans-induced apoptosis in human gingival epithelial cells. In addition, irsogladine maleate, amphotericin B, azithromycin, and Houttuynia cordata down-regulated A. actinomycetemcomitans-induced increase of IL-8 through ERK signaling in human gingival epithelial cells. These findings suggested that the regulation of these cascades may lead the prevention of periodontal disease　by regulating the function of gingival epithelium.

Research Products

• [Journal Article] Houttunia Cordata suppressed the Aggregatibacter actinomycetemcomitans-induced increase of inflammatory-related genes in cultured human gingival epithelial cells. (2015)
  • Author(s): Kabir MA., Fujita T., Ouhara K., Kajiya M., Matsuda S., Shiba H., Kurihara H.
  • Journal Title: Journal of Dental Sciences Volume: 10 Pages: 88-94
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Irsogladine maleate inhibits Porphyromonas gingivalis-mediated expression of toll-like receptor 2 and interleukin-8 in human gingival epithelial cells. (2015)
  • Author(s): Savitri IJ., Ouhara K., Fujita T., Kajiya M., Miyagawa T., Kittaka M., Yamakawa M., Shiba H., Kurihara H.
  • Journal Title: Journal of Periodontal Research Volume: 印刷中 Issue: 4 Pages: 486-493
  • DOI: 10.1111/jre.12231
  • Peer Reviewed
• [Journal Article] Amphotericin B down-regulates Aggregatibacter actinomycetemcomitans-induced production of IL-8 and IL-6 in human gingival epithelial cells. (2014)
  • Author(s): Imai H., Fujita T., Kajiya M., Ouhara K., Miyagawa T., Matsuda S., Shiba H., Kurihara H.
  • Journal Title: Celler Immunology Volume: 290 Issue: 2 Pages: 201-208
  • DOI: 10.1016/j.cellimm.2014.07.001
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Smad2 is involved in Aggregatibacter actinomycetemcomitans-induced apoptosis. (2014)
  • Author(s): Yoshimoto T., Fujita T., Ouhara K., Kajiya M., Imai H., Shiba H., Kurihara H.
  • Journal Title: Journal of Dental Research Volume: 93 Issue: 11 Pages: 1148-1154
  • DOI: 10.1177/0022034514550041
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Irsogladine maleate regulates the inflammatory related genes in human gingival epithelial cells stimulated by Aggregatibacter actinomycetemcomitans. (2013)
  • Author(s): Miyagawa T
  • Journal Title: Int Immunopharmacol Volume: 15 Issue: 2 Pages: 340-347
  • DOI: 10.1016/j.intimp.2012.12.011
  • Peer Reviewed
• [Journal Article] Smad2 is involved in the apoptosis of murine gingival junctional epithelium associated with inhibition of Bcl-2. (2012)
  • Author(s): Fujita T.
  • Journal Title: Arch Oral Biol. Volume: Nov;57(11) Issue: 11 Pages: 1567-73
  • DOI: 10.1016/j.archoralbio.2012.08.011
  • Peer Reviewed
• [Presentation] ヒト歯肉上皮細胞におけるバクテリアによるアポトーシスとsmad2との関与 (2014)
  • Author(s): 吉本哲也，藤田 剛， 柴 秀樹， 栗原 英見
  • Organizer: 第56回歯科基礎医学会学術大会・総会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] TGF-β1が歯肉上皮細胞のアポトーシスシグナル，E-cadherinに与える影響 (2013)
  • Author(s): 吉本哲也, 藤田 剛, 應原一久, 加治屋幹人, 宮川剛史, 今井遙香, 柴 秀樹, 栗原英見
  • Organizer: 第56回春季日本歯周病学会学術大会
  • Place of Presentation: 東京
• [Presentation] 歯肉上皮細胞に着目した歯周炎発症メカニズムの解明とその制御 (2013)
  • Author(s): 藤田 剛
  • Organizer: 第56回春季日本歯周病学会学術大会
  • Place of Presentation: 東京
  • Invited
• [Presentation] ヒト歯肉上皮細胞は TGF-β type I Receptor-smad2-caspase3/9シグナル伝達経路を介してアポトーシスを生じる (2013)
  • Author(s): 吉本哲也, 藤田 剛, 應原一久, 加治屋幹人, 宮川剛史, 今井遙香, 柴 秀樹, 栗原英見
  • Organizer: 第139回日本歯科保存学会2013年度秋季学術大会
  • Place of Presentation: 秋田
• [Presentation] Aggregatibacter actynomycetemcomitansのouter membrane protein-29はヒト歯肉上皮細胞においてsmad2を介してアポトーシスシグナルを誘導し，E-cadherinの発現を減少させる (2012)
  • Author(s): 吉本哲也，藤田 剛，應原一久，内田雄士，松田真司，宮川剛史，今井遥香，柴 秀樹，河口浩之，栗原英見
  • Organizer: 平成24年度春季学術大会（第136回）
  • Place of Presentation: 沖縄コンベンションセンター