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Investigation of bacterial distribution in pregnant women by metagenomics analysis and prevention of adverse pregnancy outcomes

Research Project

Project/Area Number 24593130
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Periodontal dentistry
Research InstitutionHealth Sciences University of Hokkaido

Principal Investigator

KATO Satsuki  北海道医療大学, 歯学部, 講師 (50281283)

Co-Investigator(Kenkyū-buntansha) MORI Mari  北海道医療大学, 歯学部, 講師 (30275490)
NAGASAWA Toshiyuki  北海道医療大学, 歯学部, 教授 (90262203)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsメタゲノム解析 / 歯周病 / 妊娠女性 / 早産・低体重児出産 / 妊娠 / 歯周病原細菌 / 性周期 / 妊産婦
Outline of Final Research Achievements

Previous studies have suggested that periodontal disease might be a risk factor for preterm birth and/or low birth weight. The aim of this study was to evaluate the association between oral bacteria and systemic conditions in pregnant women. Salivary samples were collected from pregnant women and non-pregnant women, and periodontal examination was performed. Concentrations of progesterone and estradiol were determined using ELISA. The bacterial DNA was isolated from saliva, and the bacterial composition of oral bacterium was investigated by 16S rRNA-based metagenomics analysis. It was found that progesterone levels in pregnant women were significantly higher than non-pregnant women. From 16S rRNA-based metagenomics analysis, Bifidobacteriales was only emerged in pregnant women. Periodontal status was not significantly different between pregnant women and non-pregnant women. These results suggest that Bifidobacteriales might be increased in pregnant women.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (6 results)

All 2015 2014 2013 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 1 results) Presentation (3 results)

  • [Journal Article] Host-microbialco-evolutioninperiodontitisassociatedwithAggregatibacteractinomycetemcomitansinfection.2014

    • Author(s)
      NagasawaT,ShimizuS,KatoS,NakatsukaY,KadoT,HidakaT,ShiraiK,MoriM,andFuruichi
    • Journal Title

      JournalofOralBiosciences,

      Volume: 58 Issue: 1 Pages: 11-17

    • DOI

      10.1016/j.job.2013.10.002

    • Related Report
      2014 Annual Research Report 2013 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Involvement of Toll-like receptor 2 in apoptosis of Aggregatibacter actinomycetemcomitans-infected THP-1 cells2013

    • Author(s)
      Kato S.
    • Journal Title

      J Microbiol Immunol Infect

      Volume: 46 Issue: 3 Pages: 164-170

    • DOI

      10.1016/j.jmii.2012.02.003

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Involvement of Toll-like receptor 2 in apoptosis of Aggregatibacter actinomycetemcomitans-infected THP-1 cells.2013

    • Author(s)
      Satsuki Kato、 Keisuke Nakashima、 Toshiyuki Nagasawa、 Yoshihiro Abiko、 Yasushi Furuichi
    • Journal Title

      Journal of Microbiology, Immunology and Infection

      Volume: 未定

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] 妊娠女性における唾液のメタゲノム解析2015

    • Author(s)
      加藤幸紀、長澤敏行、古市保志
    • Organizer
      北海道医療大学歯学会第33回学術大会
    • Place of Presentation
      北海道医療大学札幌サテライトキャンパス
    • Year and Date
      2015-03-07
    • Related Report
      2014 Annual Research Report
  • [Presentation] 北海道在住の日本人家族から分離されたJP2タイプのA. actinomycetemcomitansの特徴

    • Author(s)
      長澤敏行、清水伸太郎、加藤幸紀、門 貴司、日高竜宏、森 真理、古市保志
    • Organizer
      日本歯周病学会
    • Place of Presentation
      札幌コンベンションセンター
    • Related Report
      2012 Research-status Report
  • [Presentation] JP2型leukotoxin promoterを有するA. actinomycetemcomitans菌の解析

    • Author(s)
      清水伸太郎、長澤敏行、門 貴司、日高竜宏、加藤幸紀、森 真理、古市保志
    • Organizer
      日本歯周病学会
    • Place of Presentation
      札幌コンベンションセンター
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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