Genome sequencing of clinical strains isolated from dental plaque for investigation of polymicrobial factors in periodontal pathogenesis

• Project/Area Number: 24593135
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Periodontal dentistry
• Research Institution: Aichi Gakuin University
• Principal Investigator: HAYASHI Jun-ichiro 愛知学院大学, 歯学部, 講師 (30350937)
• Co-Investigator(Kenkyū-buntansha): MORITA Hidetoshi 麻布大学, 獣医学部, 教授 (70957294) ; ISHIHARA Yuichi 松本歯科大学, 歯学部, 教授 (50261011) ; KAMEI Hidehiko 愛知学院大学, 歯学部, 講師 (50421243) ; 森田 英利 麻布大学, 獣医学部, 教授 (70257294)
• Co-Investigator(Renkei-kenkyūsha): HATTORI Masahira 東京大学, 新領域創成科学研究科, 教授 (70175537)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 歯周病 / プラーク細菌叢 / 臨床株 / 16SrRNA解析 / ゲノム解析 / う蝕細菌 / プラーク細菌

Outline of Final Research Achievements

To unravel the complex polymicrobial mechanisms involved in periodontitis, it is necessary to enhance the reference informations on the public databases for evaluation the pathogenic potential of the whole community. The aim of this study was to isolate clinical strains including novel species from dental plaque of patients with periodontitis and to determine the genome sequences. Seventeen strains that showed 16S homology of less than 98.7% to known species were isolated and three of those with particularly low homology were determined draft genome sequences as candidates of novel strain. On the other hands, complete genome sequencing of three known stains isolated dental caries, B. dentium JCM 1195, S. inopinata JCM 12537 and P. denticolens JCM 12538, was also performed. These strains are classified under the family bifidobaceriaceae. The sequence data for the genome have been deposited in DDBJ/GenBank/EMBL under the accession no AP012326, AP012334 and AP012333, respectively.

Research Products

• [Journal Article] Complete genome sequence of Bifidobacterium dentium JCM 1195T isolated from human dental caries (2015)
  • Author(s): Hidehiro Toh, Jun-ichiro Hayashi, Kenshiro Oshima, Akiyo Nakano, Yukiko Takayama, Kageyasu Takanashi, Hidetoshi Morita, and Masahira Hattori
  • Journal Title: Genome Announcements Volume: in press
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Complete genome sequence of Scardovia inopinata JCM 12537T isolated from human dental caries (2015)
  • Author(s): Kenshiro Oshima, Jun-ichiro Hayashi, Hidehiro Toh, Akiyo Nakano, Emi Omori, yasue Hattori, Hidetoshi Morita, Kenya Honda, and Masahira Hattori.
  • Journal Title: Genome Announcements Volume: in press
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Complete genome sequence of Parascardovia denticolens JCM 12538T isolated from human dental caries (2015)
  • Author(s): Kenshiro Oshima, Jun-ichiro Hayashi, Hidehiro Toh, Akiyo Nakano, Chie Shindo, Keiko Komiya, Hidetoshi Morita, Kenya Honda, and Masahira Hattori.
  • Journal Title: Genome Announcements Volume: in press
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] T-RFLP法による咽頭領域の細菌叢解析と口臭との関連 (2014)
  • Author(s): 岩村侑樹, 福田光男, 三谷章雄, 林潤一郎, 佐藤聡太, 高橋伸行, 藤村岳樹, 岡田康佑, 村上多惠子, 佐藤孝至, 嶋﨑義浩, 野口俊英
  • Organizer: 第57回春季日本歯周病学会
  • Place of Presentation: 岐阜市
  • Year and Date: 2014-05-23 – 2014-05-24