Assessment of transmission between oral and tonsillar microbiota by T-RFLP analysis based on oral malodor

• Project/Area Number: 24593136
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Periodontal dentistry
• Research Institution: Aichi Gakuin University
• Principal Investigator: FUKUDA Mitsuo 愛知学院大学, 歯学部, 教授 (40156790)
• Co-Investigator(Kenkyū-buntansha): NOGUCHI Toshihide 愛知学院大学, 歯学部, 教授 (50014262) ; MITANI Akio 愛知学院大学, 歯学部, 教授 (50329611)
• Co-Investigator(Renkei-kenkyūsha): SATO Takashi 愛知学院大学, 歯学部, 准教授 (00387573)
• Research Collaborator: HAYASHI Jun-ichiro 愛知学院大学, 歯学部, 講師 (30350937) ; SATO Sato 愛知学院大学, 歯学部, 非常勤助教 (40647054)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
• Keywords: 細菌叢 / 歯周病 / 口臭

Outline of Final Research Achievements

We focused on tonsil as the source of the re-infection of periodontal pathogen based on oral malodor. Malodor is thought to be early sign of periodontal disease. Orally healthy patients were assigned to one of three groups. The volatile sulfur compounds in mouth air were assessed before and after gargling. For bacterial assessment, biofilm was got from the palatine tonsils. VSC-producing periodontal pathogens were detected in the samples and the bacterial profiles were analyzed by the T-RFLP. Clone libraries of two high malodor patients were sequenced for the T-RFs. Gargling reduced VSCs and the organoleptic score, but did not affect the periodontal pathogens. The T-RFLP analysis showed no significant change in the bacterial profile after gargling, but decrease in some of the T-RFs in the test. The candidate species assigned to these T-RFs contained VSC-producing bacteria such as Veillonella and Prevotella species.

Research Products

• [Journal Article] Irradiation with a low-level diode laser induces the developmental endothelial locus-1 gene and reduces pro-inflammatory cytokines in epithelial cells (2014)
  • Author(s): Fujimura T, Mitani A, Fukuda M, Mogi M, Osawa K, Takahashi S, Aino M, Iwamura Y, Miyajima S, Yamamoto H, Noguchi T
  • Journal Title: Lasers Med Sci Volume: 29 Issue: 3 Pages: 987-994
  • DOI: 10.1007/s10103-013-1439-6
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Five-year clinical results for treatment of intrabony defects with EMD, guided tissue regeneration and open-flap debridement: a case series (2014)
  • Author(s): Mitani A,Takasu H,Horibe T, Furuta H, Nagasaka T, Aino M, Fukuda M, Fujimura T, Mogi M, Noguchi T.
  • Journal Title: J Periodont Res Volume: 印刷中 Issue: 1 Pages: 123-130
  • DOI: 10.1111/jre.12188
  • Peer Reviewed / Open Access
• [Journal Article] Morphological Alterations of Periodontal Pocket Epithelium Following Nd:YAG Laser Irradiation (2014)
  • Author(s): Ting CC, Fukuda M, Watanabe T, Sanaoka A, Mitani A, Noguchi T
  • Journal Title: Photomed Laser Surg Volume: 32 Issue: 12 Pages: 649-657
  • DOI: 10.1089/pho.2014.3793
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Nd:YAG laser irradiation of the tooth root surface inhibits demineralization and root surface softening caused by minocycline application (2013)
  • Author(s): Watanabe T, Fukuda M, Mitani A, Ting CC, Osawa K, Nagahara A, Sato S, Fujimura T, Takahashi S, Iwamura Y, Murakami T, Noguchi T.
  • Journal Title: Photomed Laser Surg Volume: 31 Issue: 12 Pages: 571-577
  • DOI: 10.1089/pho.2013.3561
  • Peer Reviewed
• [Journal Article] Follicular dendritic cell-secreted protein expression is reduced in experimental periodontitis concurrently with the up-regulation of IL-17 mRNA and an increase in the RANKL/OPG mRNA ratio (2013)
  • Author(s): Takahashi S, Fukuda M, Mitani A, Fujimura T, Iwamura Y, Sato S, Kubo T, Sugita Y, Maeda H, Shinomura T, Noguchi T.
  • Journal Title: J Periodont Res Volume: 印刷中 Issue: 3 Pages: 390-397
  • DOI: 10.1111/jre.12118
  • Peer Reviewed / Open Access
• [Journal Article] Nd:YAG Laser Irradiation of the Tooth Root Surface Inhibits Demineralization and Root Surface Softening Caused by Minocycline Application. (2013)
  • Author(s): Watanabe T, Fukuda M, Mitani A, Ting C-C, Osawa K, Nagahara A, Satoh S, Fujimura T, Takahashi S, Iwamura Y, Murakami T, Noguchi T.
  • Journal Title: Photomed Laser Surg. Volume: 31 Pages: 571-517
  • Peer Reviewed
• [Journal Article] 口臭への対応と口臭症治療 (2013)
  • Author(s): 角田正健，喜多成价，久保伸夫，角田博之，福田光男，本田俊一
  • Journal Title: におい・かおり環境学会誌 Volume: ４４ Pages: 230-237
  • NAID: 130006164734
• [Journal Article] 歯学部・歯科大学における女性歯科医師の就業状況および支援制度の利用状況 (2013)
  • Author(s): 村上多惠子，森榮，井上峰雄，加藤一夫，福田光男，野口俊英
  • Journal Title: 日本口腔衛生会誌 Volume: 63 Pages: 444-452
  • NAID: 110009675858
  • Peer Reviewed
• [Journal Article] Antimicrobial photodynamic therapy using a diode laser with a potential new photosensitizer, indocyanine green-loaded nanospheres, may be effective for the clearance of Porphyromonas gingivalis (2013)
  • Author(s): Nagahara A, Mitani A, Fukuda M, Yamamoto H, Tahara K, Morita I, Ting CC, Watanabe T, Fujimura T, Osawa K, Sato S, Takahashi S, Iwamura Y, Kuroyanagi T, Kawashima Y, Noguchi T.
  • Journal Title: J Periodontal Res Volume: 48 Issue: 5 Pages: 591-599
  • DOI: 10.1111/jre.12042
  • Peer Reviewed
• [Presentation] 若年層の唾液シアル酸濃度の日内変動と生理的口臭について (2015)
  • Author(s): 髙橋枝里、村上多恵子、橋本洋子、藤村岳樹、岩村侑樹、佐々木康行、嶋崎義浩、金森孝雄、林潤一郎、三谷章雄、福田光男
  • Organizer: 第6回日本口臭学会学術大会
  • Place of Presentation: 新潟/日本歯科大学新潟生命歯学部
  • Year and Date: 2015-07-04 – 2015-07-05
• [Presentation] 口蓋扁桃部細菌叢と口臭との関連ー含嗽剤を用いた介入研究ー (2015)
  • Author(s): 岩村侑樹、林潤一郎、佐藤孝至、佐藤聡太、村上多恵子、藤村岳樹、佐々木康之、高橋枝里、嶋崎義浩、三谷章雄、福田光男
  • Organizer: 第6回日本口臭学会学術大会
  • Place of Presentation: 新潟/日本歯科大学新潟生命歯学部
  • Year and Date: 2015-07-04 – 2015-07-05
• [Presentation] 唾液および咽頭部細菌叢のT-RFLP 法による解析データと口臭との関連 (2014)
  • Author(s): 岩村 侑樹、福田 光男、林 潤一郎、佐藤 聡太、藤村 岳樹、高橋 枝里、佐々木 康行、村上 多惠子、佐藤 孝至、嶋崎 義浩、三谷 章雄
  • Organizer: 第5回日本口臭学会学術大会
  • Place of Presentation: 大阪/近畿大学
  • Year and Date: 2014-07-05 – 2014-07-06
• [Presentation] T-RFLP 法による咽頭領域の細菌叢解析と口臭との関連 (2014)
  • Author(s): 岩村 侑樹、福田 光男、三谷 章雄、林 潤一郎、佐藤 聡太、高橋伸行、藤村 岳樹、岡田康佑、村上 多惠子、佐藤 孝至、嶋崎 義浩、野口俊英
  • Organizer: 第57回春季日本歯周病学学会
  • Place of Presentation: 岐阜/長良川国際会議場
  • Year and Date: 2014-05-23 – 2014-05-25
• [Presentation] 「口臭への対応と口臭治療の指針」を目指して (2012)
  • Author(s): 角田正健，福田光男，久保伸夫，本田俊一，角田博之
  • Organizer: 日本口臭学会第3回学術大会
  • Place of Presentation: 東京
  • Invited
• [Presentation] レーザーを安全に使用するために忘れてはならないこと (2012)
  • Author(s): 福田光男
  • Organizer: 第12回歯科用レーザー学会
  • Place of Presentation: 東京
  • Invited
• [Presentation] 歯周病におけるFollicular Dendric Cell-Secreted Prptein(FDC-CP)の接合上皮での発現について (2012)
  • Author(s): 高橋伸行，福田光男，三谷章雄，佐藤聡太，藤村岳樹，渡辺智久，永原絢子，大澤数洋，前初彦，杉田好，篠村多摩之，大城暁子，野口俊英．
  • Organizer: 第55回日本歯周病学学会春季学術大会
  • Place of Presentation: 札幌
• [Presentation] 半導体レーザー軟組織透過性に関する基礎的検討 (2012)
  • Author(s): 岩村侑樹，福田光男，三谷章雄，大澤数洋，永原絢子，山本浩充，佐藤聡太，高橋伸行，藤村岳樹，須賀貴行，渡辺智久，佐々公太，野口俊英
  • Organizer: 第24回日本レーザー歯学会学術大会
  • Place of Presentation: 神戸
• [Presentation] 垂直性骨欠損に対しGTR 法とエナメルマトリックスタンパク質を用いた歯周組織再生療法を行った症例の５年経過時における臨床的比較
  • Author(s): 相野誠，三谷章雄，高須英成，古田英豊，永坂太郎，三輪晃資，菊池毅，川瀬仁史，伊藤正満，林潤一郎，黒須康成，石原裕一，稲垣幸司，福田光男，野口俊英
  • Organizer: 第138 回日本歯科保存学会春季学術大会
  • Place of Presentation: 福岡
• [Presentation] ICG ナノ粒子による軟組織透過エネルギー吸収の基礎的検討
  • Author(s): 岡田康佑，福田光男，三谷章雄，山本浩充，西田栄作，佐藤颯太，高橋伸行，藤村岳樹，岩村侑樹，須賀貴行，渡辺智久，佐々公太，大澤数洋，永原絢子，河合達志，野口俊英
  • Organizer: 第２５回レーザー歯学会総会・学術大会
  • Place of Presentation: 東京
• [Presentation] インドシアニングリーン封入ナノ粒子が半導体レーザー照射によるエネルギー吸収・温度変化に与える影響 〜ポケット外照射モデルを用いた基礎的検討〜
  • Author(s): 岩村侑樹，福田光男，三谷章雄，山本浩充，西田英作，佐藤聡太，高橋伸行，藤村岳樹，岡田康佑，河合達志，野口俊英
  • Organizer: 第81 回愛院大歯学会
  • Place of Presentation: 名古屋
• [Book] Manual for oral care (2015)
  • Author(s): Chif. Edit.:Nagato Natsume, Co Edit; Mistuo Fukuda et al.
  • Total Pages: 346
  • Publisher: Quintessence Publishing Co.
• [Book] 口臭への対応と口臭治療の指針2014 (2015)
  • Author(s): 角田正健、喜多成价、久保伸夫、角田博之、福田光男、本田俊一、宮地英雄
  • Total Pages: 28
  • Publisher: 日本口臭学会