| Project/Area Number |
24593147
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Social dentistry
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| Research Institution | Osaka University |
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Principal Investigator |
MAEDA Kazuhiko 大阪大学, 歯学研究科(研究院), 助教 (00346165)
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| Co-Investigator(Kenkyū-buntansha) |
KUBONIWA Masae 大阪大学, 歯学部附属病院, 講師 (00303983)
NAGATA Hideki 大阪大学, 大学院歯学研究科, 准教授 (50260641)
SEKINE Shinichi 大阪大学, 大学院歯学研究科, 助教 (70506344)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | リン酸化タンパク質 / バイオフィルム / 歯周病 / S. oralis / P. gingivalis / S. gordonii / ショットガンプロテオミクス / 口腔レンサ球菌 / バイオマーカー / プロテオーム / リン酸化 / S. oralis |
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| Outline of Final Research Achievements |
Porphyromonas gingivalis, a major periodontal pathogen, forms biofilm with other oral bacteria such as streptococci. Here, using shotgun proteomics we examined the molecular basis of mixed-biofilm formation by P. gingivalis with Streptococcus oralis. We identified a total of 593 bacterial proteins in the biofilm. Compared to the expression profile in the P. gingivalis mono-biofilm, the expression of 3 proteins was induced and that of 31 proteins was suppressed in the mixed biofilm. Additionally, the expression of 2 S. oralis proteins was increased while that of 2 proteins was decreased in the mixed biofilm, as compared to its monotypic profile. These findings suggest that several functional molecules are involved in biofilm formation between P. gingivalis and S. oralis.
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