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Roles of plant extracts in therapeutic and preventive agents for periodontitis

Research Project

Project/Area Number 24593172
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Social dentistry
Research InstitutionKanagawa Dental College

Principal Investigator

WATANABE Kiyoko  神奈川歯科大学, 歯学研究科(研究院), 講師 (70148021)

Co-Investigator(Kenkyū-buntansha) HAMADA Nobushiro  神奈川歯科大学, 大学院歯学研究科, 教授 (20247315)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords植物由来ポリフェノール / 歯周炎 / 抗菌活性 / 歯槽骨吸収抑制効果 / 付着・定着阻害 / 口臭抑制 / 唾液細菌 / 歯周健康 / 抗菌効果 / 破骨細胞分化誘導抑制
Outline of Final Research Achievements

We examined the inhibitory effects of Pycnogenol (PYC) on alveolar bone resorption induced by P. gingivalis and osteoclast differentiation. In rat periodontitis model, rats were fed a regular diet or PYC mixed diet (0.025%: w/w). Administration of PYC along with P. gingivalis infection significantly reduced alveolar bone resorption. Treatment of P. gingivalis with 1 μg/ml PYC reduced the number of viable bacterial cells. Addition of PYC to epithelial cells inhibited adhesion and invasion by P. gingivalis. PYC treatment significantly inhibited osteoclast formation. Addition of PYC (100 μg/ml, 10 μg/ml or 1 μg/ml) to purified osteoclasts culture induced cell apoptosis. These results suggest that PYC may prevent alveolar bone resorption through its antibacterial activity against P. gingivalis and by suppressing osteoclastogenesis. Therefore, PYC may be useful as a therapeutic and preventative agent for bone diseases such as periodontitis.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (12 results)

All 2015 2014 2013 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (9 results)

  • [Journal Article] Inhibitory effects of French pine bark extract, Pycnogenol®, on alveolar bone resorption and on the osteoclast differentiation.2015

    • Author(s)
      Sugimoto H, Watanabe K, Toyama T, Takahashi SS, Sugiyama S, Lee MC, Hamada N
    • Journal Title

      Phytotherapy Research

      Volume: 29 Issue: 2 Pages: 251-259

    • DOI

      10.1002/ptr.5245

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Ameliorating effects of Juzentaihoto on restraint stress and P. gingivalis-induced alveolar bone loss.2014

    • Author(s)
      Takeda O, Toyama T, Watanabe K, Sato T, Sasaguri K, Akimoto S, Sato S, Kawata T, Hamada N
    • Journal Title

      Archives of Oral Biology

      Volume: 59 Pages: 1130-1138

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Reactive oxygen species scavenging activity of Jixueteng evaluated by electron spin resonance (ESR) and photon emission.2014

    • Author(s)
      Toyama T, Wada-Takahashi S, Takamichi M, Watanabe K, Yoshida A, Yoshino F, Miyamoto C, Maehata Y, Sugiyama S, Takahashi SS, Todoki K, Lee MC, Hamada N
    • Journal Title

      Natural Product Communications

      Volume: 9 Pages: 1755-1759

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Presentation] Pycnogenol gum reduces gingivitis, plaque formation and oral malodor.2014

    • Author(s)
      Hamada N, Watanabe K
    • Organizer
      7th World Congress on Preventive & Regenerative Medicine
    • Place of Presentation
      Taipei
    • Year and Date
      2014-11-04 – 2014-11-07
    • Related Report
      2014 Annual Research Report
  • [Presentation] Antioxidant effects of Jixueteng.2014

    • Author(s)
      Toyama T, Todoki K, Matsuo M, Takahashi S-S, Wada-Takahashi S, Takamichi M, Yoshino F, Yoshida A, Watanabe K, Hamada N
    • Organizer
      7th World Congress on Preventive & Regenerative Medicine
    • Place of Presentation
      Taipei
    • Year and Date
      2014-11-04 – 2014-11-07
    • Related Report
      2014 Annual Research Report
  • [Presentation] ピクノジェノール含有ガムの口臭抑制効果の検討.2014

    • Author(s)
      渡辺清子,浜田信城
    • Organizer
      神奈川歯科大学学会第145回例会
    • Place of Presentation
      広島
    • Year and Date
      2014-10-25
    • Related Report
      2014 Annual Research Report
  • [Presentation] フランス海岸松抽出成分含有ガムの口臭抑制効果の検討.2014

    • Author(s)
      渡辺清子,浜田信城
    • Organizer
      第56回歯科基礎医学会学術大会・総会
    • Place of Presentation
      福岡
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] マウス実験的歯周炎モデルにおける生薬「鶏血藤」の循環改善効果.2014

    • Author(s)
      遠山歳三,塗々木和男,高橋俊介,吉野文彦,吉田彩佳,松尾雅斗,高橋聡子,渡辺清子,浜田信城
    • Organizer
      第56回歯科基礎医学会学術大会・総会
    • Place of Presentation
      福岡
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] Effects of pine bark extract chewing gum on oral malodor.2014

    • Author(s)
      Watanabe K, Hamada N
    • Organizer
      92nd General Session and Exhibition of the IADR
    • Place of Presentation
      Cape Town
    • Year and Date
      2014-06-23 – 2014-06-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] 十全大補湯のストレス負荷とP. gingivalis感染ラットにおける歯槽骨吸収に対する改善効果.2014

    • Author(s)
      武田織英,遠山歳三,渡辺清子,佐藤武則,笹栗健一,秋本進,佐藤貞雄,河田俊嗣,浜田信城
    • Organizer
      神奈川歯科大学学会第144回例会
    • Place of Presentation
      横須賀
    • Year and Date
      2014-06-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] Ameliorating effect of Jixueteng on Porphyromonas gingivalis-induced alveolar bone loss in mice.2013

    • Author(s)
      Toyama T, Sato T, Todoki K, Matsuo M, Watanabe K, Takahashi S, Lee MC, Hamada N
    • Organizer
      99th Annual Meeting of American Academy of Periodontology
    • Place of Presentation
      Philadelphia
    • Related Report
      2013 Research-status Report
  • [Presentation] ピクノジェノールの破骨細胞分化誘導に対する阻害効果の検討

    • Author(s)
      渡辺清子
    • Organizer
      神奈川歯科大学学会 第47回総会
    • Place of Presentation
      神奈川歯科大学(横須賀)
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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