New concept of periodontal disease as a life-style related disease

• Project/Area Number: 24593174
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Social dentistry
• Research Institution: Tsurumi University
• Principal Investigator: NOMURA Yoshiaki 鶴見大学, 歯学部, 准教授 (90350587)
• Co-Investigator(Kenkyū-buntansha): HANADA Nobuhiro 鶴見大学, 歯学部, 教授 (70180916) ; SHIMODA Shinji 鶴見大学, 歯学部, 教授 (30139620)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 歯周病 / 生活習慣病 / 歯石 / 歯石形成 / 石灰化

Outline of Final Research Achievements

We established the artificial dental calculus formation system and investigated the effect of ox-LDL for the formation of dental calculous. By the SEM analysis, clearly distinguished crystals were observed when membrane was immersed in HDL. By the TEM analysis, Hydroxyapatite like crystals was observed when Ca solutions were mixed with LDL or ox-LDL. And Ca/P of the crystals with ox-LDL was highest when compared with LDL, HDL. These results indicated that ox-LDL promote the dental calculus formation.

Research Products

• [Journal Article] Cranial-base morphology in adults with skeletal Class III malocclusion. (2014)
  • Author(s): Sanggarnjanavanich S, Sekiya T, Nomura Y, Nakayama T, Hanada N, Nakamura Y.
  • Journal Title: Am J Orthod Dentofacial Orthop. Volume: 146(1) Issue: 1 Pages: 82-91
  • DOI: 10.1016/j.ajodo.2014.04.014
  • Peer Reviewed
• [Journal Article] Function of chemokine (CXC motif) ligand 12 in periodontal ligament fibroblasts. (2014)
  • Author(s): Yashiro Y, Nomura Y, Kanazashi M, Noda K, Hanada N, Nakamura Y.
  • Journal Title: PLoS One. Volume: 7;9(5) Issue: 5 Pages: e95676-e95676
  • DOI: 10.1371/journal.pone.0095676
  • Peer Reviewed
• [Journal Article] Correlation between health-care costs and salivary tests. (2013)
  • Author(s): Kakuta E, Nomura Y, Naono Y, Koresawa K, Shimizu K, Hanada N.
  • Journal Title: Int Dent J. Volume: Oct;63(5) Issue: 5 Pages: 249-53
  • DOI: 10.1111/idj.12040
  • Peer Reviewed
• [Journal Article] Distributionof Streptococcus troglodytae and Streptococcus dentirousetti in chim- panzee oral cavities. (2013)
  • Author(s): Miyanohara M, Imai S, Okamoto M, Saito W, Nomura Y, Momoi Y, Tomonaga M, Hanada N.
  • Journal Title: Microbiology and Immunology, in press Volume: [Epub ahead of print] Issue: 5 Pages: 359-65
  • DOI: 10.1111/1348-0421.12047
  • Peer Reviewed
• [Journal Article] Assessment of the plasma/serum IgG test to screen for periodontitis. (2012)
  • Author(s): Kudo C
  • Journal Title: J Dent Res Volume: 91 Issue: 12 Pages: 1190-1195
  • DOI: 10.1177/0022034512461796
  • Peer Reviewed
• [Journal Article] Salivary biomarkers for predicting the progression of chronic periodontitis (2012)
  • Author(s): Nomura Y、Inagaki K、8th 10 others
  • Journal Title: Arch Oral Biol Volume: Vol. 57、No. 4 Issue: 4 Pages: 413-420
  • DOI: 10.1016/j.archoralbio.2011.09.011
  • Peer Reviewed
• [Journal Article] Human periodontal ligament fibroblasts are the optimal cell source for induced pluripotent stem cells (2012)
  • Author(s): Nomura Y, Ishikawa M, Yashiro Y, Sanggarnjanavanich S, Yamaguchi T, Arai C, Noda K, Takano Y, Nakamura Y, Hanada N
  • Journal Title: Histochem Cell Biol.2012 Volume: (Epub ahead of print) Issue: 6 Pages: 719-732
  • DOI: 10.1007/s00418-012-0923-6
  • Peer Reviewed
• [Journal Article] Streptococcus troglodytae sp.nov., from the Chimpanzee Oral Cavity (2012)
  • Author(s): Okamoto M, Imai S, Miyanohara M, Saito W, Momoi Y, Abo T, Nomura Y, Ikawa T, Ogawa T, Miyabe-Nishiwaki T, Kaneko A, Watanabe A, Watanabe S, Hayashi M, Tomonaga M, Hanada N
  • Journal Title: Int J Syst Evol Microbiol Volume: (Epub ahead of print) Issue: Pt_2 Pages: 418-22
  • DOI: 10.1099/ijs.0.039388-0
  • Peer Reviewed
• [Journal Article] Quantification of periodontopathic bacteria in saliva using the invader assay. (2012)
  • Author(s): Tada A, Takeuchi H, Shimizu H, Tadokoro K, Tanaka K, Kawamura K, Yamaguchi T, Egashira T, Nomura Y, Hanada N.
  • Journal Title: Jpn J Infect Dis. Volume: 65(5) Pages: 415-423
  • NAID: 130001931851
  • Peer Reviewed
• [Presentation] 菌血症に関連する口腔バイオフィルム細菌の検討 (2012)
  • Author(s): 武内 博朗, 村田 貴俊, 角田 衣理加, 野村 義明, 泉福 英信, 花田 信弘
  • Organizer: 日本口腔感染症学会総会
  • Place of Presentation: 横浜