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Analysis of gram-positive/negative bacteria ratio and pathogenicity of oral biofilm

Research Project

Project/Area Number 24593176
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Social dentistry
Research InstitutionTsurumi University

Principal Investigator

TAKEUCHI HIROAKI  鶴見大学, 歯学部, 臨床教授 (50572260)

Co-Investigator(Kenkyū-buntansha) SENPUKU Hidenobu  国立感染症研究所, 細菌第6室, 室長 (20250186)
NOMURA Yoshiaki  鶴見大学, 歯学部, 准教授 (90350587)
HANADA Nobuhiro  鶴見大学, 歯学部, 教授 (70180916)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords口腔バイオフィルム / エンドトキシン / グラム陽性菌 / グラム陰性菌 / 3DS / 歯周病 / 菌血症 / 口腔保健 / デンタルプラーク / 細菌 / LPS / 抗LPS抗体 / FISH / Porphyromonas gingivalis
Outline of Final Research Achievements

Periodontal treatments including the antibacterial therapy for periodontitis patients (n=116) resulted in the significant reduction of P. gingivalis ratio, while the number of the total bacteria did not change. The study of the fluorescence insitu hybridization (FISH) also showed that the signal of P.gingivalis decreased and the signal of total streptococci increased. In addition, peripheral blood of the severe periodontitis patients(n=12) showed that 10% were bacteremia-positive before scaling, 20% for just after scaling, and 50% for 10 minutes after scaling. While after periodontal treatment including antibacterial therapy, the periferal blood of three of five patients turned bacteremia-negative for even just after scaling.
These results suggest that oral bacterial control with the periodontal treatment leads to the risk reduction of systemic disease including odontogenic bacteremia and endotoxinemia, as well as oral diseases.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (12 results)

All 2014 2013 2012

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (7 results) (of which Invited: 2 results) Book (4 results)

  • [Journal Article] スケーリング時の歯原性菌血症制御に関する細菌学的解析2014

    • Author(s)
      武内博朗、花田信弘
    • Journal Title

      日本アンチエイジング歯科学会誌

      Volume: 7 Pages: 22-27

    • NAID

      40020277610

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Presentation] Investigation on the control of odontogenic bacteremia2014

    • Author(s)
      武内博朗、村田孝俊、角田衣理加、野村義明、泉福英信、花田信弘
    • Organizer
      第97回日本細菌学会関東支部総会
    • Place of Presentation
      東京ドームホテル(東京都文京区)
    • Year and Date
      2014-10-01
    • Related Report
      2014 Annual Research Report
  • [Presentation] 咀嚼機能と健康回復の科学的解析2014

    • Author(s)
      武内博朗
    • Organizer
      第32回日本顎咬合学会
    • Place of Presentation
      東京国際フォーラム(東京都千代田区)
    • Year and Date
      2014-06-14 – 2014-06-15
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 口腔保健の第二指標を用いた全身の健康づくり2014

    • Author(s)
      武内博朗
    • Organizer
      第63回日本口腔衛生学会総会
    • Place of Presentation
      熊本市民ホール、崇城大学ホール(熊本県熊本市)
    • Year and Date
      2014-05-29 – 2014-05-31
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 口腔の健康に関する微生物学的エンドポイントの再考2013

    • Author(s)
      武内博朗、村田貴俊、角田衣理加、野村義明、泉福英信、花田信弘
    • Organizer
      第62回日本口腔衛生学会総会
    • Place of Presentation
      キッセイ文化ホール(長野県)
    • Related Report
      2013 Research-status Report
  • [Presentation] Investigation on the control of odontogenic bacteremia2013

    • Author(s)
      H. TAKEUCHI, T. MURATA, E. KAKUTA, Y. NOMURA, H. SENPUKU, N. HANADA
    • Organizer
      10th IADR World Congress on Preventive Dentistry
    • Place of Presentation
      the Novotel Budapest City Hotel and Congress Center(ハンガリー)
    • Related Report
      2013 Research-status Report
  • [Presentation] 口腔の健康に関する微生物学的エンドポイントの再考2013

    • Author(s)
      武内博朗
    • Organizer
      日本口腔衛生学会
    • Place of Presentation
      長野県松本市 キッセイ文化ホール(長野県松本文化会館)
    • Related Report
      2012 Research-status Report
  • [Presentation] 菌血症に関連する口腔バイオフィルム細菌の検討2012

    • Author(s)
      花田信弘
    • Organizer
      日本口腔感染症学会
    • Place of Presentation
      熊本県熊本市 熊本県歯科医師会館
    • Related Report
      2012 Research-status Report
  • [Book] 歯原性菌血症を防ぐ 3DSセラピーガイドブック2014

    • Author(s)
      浦口昌秀・武内博朗
    • Total Pages
      28
    • Publisher
      デンタルダイヤモンド社
    • Related Report
      2014 Annual Research Report
  • [Book] 歯原性菌血症を防ぐ3DSセラピーガイドブック2014

    • Author(s)
      監修:花田信弘 著:浦口昌秀、武内博朗
    • Total Pages
      27
    • Publisher
      デンタルダイヤモンド社
    • Related Report
      2013 Research-status Report
  • [Book] 労働安全衛生かながわ「歯科領域が担う健康づくりと抗加齢」2013

    • Author(s)
      武内博朗
    • Total Pages
      2
    • Publisher
      (公社)神奈川労務安全衛生協会
    • Related Report
      2013 Research-status Report
  • [Book] 歯科医療ナビゲーション今さら聞け!ないこんなこと24:アンチエイジング2013

    • Author(s)
      武内博朗
    • Total Pages
      10
    • Publisher
      一般財団法人口腔保健協会 編集:升谷滋行、橋本光二、飯野文彦
    • Related Report
      2013 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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