| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
The chloric gas and phosgene are used widely in the chemical industry field. There are also more possibilities that these are used by terrorism as a chemical weapon. However, determination method of exposure by these toxic gases is not established.
Model protein was exposed to a sodium hypochlorite and digested by the proteases.
The digested peptide mixture was injected to LC-MS/MS. To compare the peak area of exposed and non-exposed samples, a few peptides that peak area decreases significantly were found. LC-MS/MS analysis was carried out using the mass number which presumed chlorine to have combined. However, we did not determine the chlorine combined peptides.
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