Search on the biomarker for the identification of Cl2 and phosgene exposure

Research Project

Project/Area Number	24612004
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Reliable environmental measurement methods
Research Institution	National Research Institute of Police Science
Principal Investigator	TSUGE Koichiro 科学警察研究所, 法科学第三部, 主任研究官 (90356204)
Co-Investigator(Kenkyū-buntansha)	OHMORI Takeshi 科学警察研究所, 室長 (70356195)
Project Period (FY)	2012-04-01 – 2015-03-31
Project Status	Completed (Fiscal Year 2014)
Budget Amount *help	¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000) Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000) Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000) Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords	塩素 / 法中毒 / 法医学 / 曝露証明 / プロテオーム / 塩素ガス / ターゲットタンパク質 / 毒性 / アダクト / ホスゲン / 曝露 / 分析 / 質量分析 / 有毒ガス
Outline of Final Research Achievements	The chloric gas and phosgene are used widely in the chemical industry field. There are also more possibilities that these are used by terrorism as a chemical weapon. However, determination method of exposure by these toxic gases is not established. Model protein was exposed to a sodium hypochlorite and digested by the proteases. The digested peptide mixture was injected to LC-MS/MS. To compare the peak area of exposed and non-exposed samples, a few peptides that peak area decreases significantly were found. LC-MS/MS analysis was carried out using the mass number which presumed chlorine to have combined. However, we did not determine the chlorine combined peptides.