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Establishment of pluripotent stem cells with low tumorigenesity by use of genomic imprinting

Research Project

Project/Area Number 24613002
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field epigenetics
Research InstitutionGunma University

Principal Investigator

HORII Takuro  群馬大学, 生体調節研究所, 助教 (00361387)

Co-Investigator(Kenkyū-buntansha) HATADA Izuho  群馬大学, 生体調節研究所, 教授 (50212147)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords単為発生 / ゲノムインプリント / 腫瘍形成 / テラトーマ / 移植医療 / ES細胞 / インプリント遺伝子 / 単為発生胚 / 腫瘍 / 再生医療
Outline of Final Research Achievements

The objective of this study is how reduce tumor formation after transplantation of ES/iPS-derived tissues. Our study has shown that parthenogenetic ES (PgES) cells are more suitable than normal diploid ES cells in the point of tumorigenesis after transplantation. Detailed analyses of DNA methylation and gene expression clarified that Snrpn gene causes tumor formation. Profiling of DNA methylation and gene expression before transplantation of ES/iPS-derived tissues will bring more safety transplantation therapy.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (16 results)

All 2014 2013 2012 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (8 results) Book (1 results) Remarks (3 results)

  • [Journal Article] Validation of microinjection methods for generating knockout mice by CRISPR/Cas-mediated genome engineering2014

    • Author(s)
      Horii T, Arai Y, Yamazaki M, Morita S, Kimura M, Itoh M, Abe Y, Hatada I
    • Journal Title

      Sci Rep

      Volume: 4 Issue: 1 Pages: 4513-4513

    • DOI

      10.1038/srep04513

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Generation of an ICF Syndrome Model by Efficient Genome Editing of Human Induced Pluripotent Stem Cells Using the CRISPR System.2013

    • Author(s)
      Horii T, Tamura D, Morita S, Kimura M, , Hatada I
    • Journal Title

      Int J Mol Sci

      Volume: 14 Issue: 10 Pages: 19774-19781

    • DOI

      10.3390/ijms141019774

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Genome engineering of mammalian haploid embryonic stem cells using the cas9/RNA system2013

    • Author(s)
      Horii T, Morita S, Kimura M, Kobayashi R, Tamura D, Takahashi RU, Kimura H, Suetake I, Ohata H, Okamoto K, Tajima S, Ochiya T, Abe Y, Hatada I
    • Journal Title

      PeerJ

      Volume: 2013 Issue: 1 Pages: e230-e230

    • DOI

      10.7717/peerj.230

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Genome-Wide Analysis of DNA Methylation and Expression of MicroRNAs in Breast Cancer Cells.2012

    • Author(s)
      Morita S, Takahashi RU, Yamashita R, Toyoda A, Horii T, Kimura M, Fujiyama A, Nakai K, Tajima S, Matoba R, Ochiya T, Hatada I.
    • Journal Title

      Int J Mol Sci.

      Volume: 13 Pages: 8259-8272

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] 半数体胚由来2倍体ES細胞の移植医療への利用の可能性2013

    • Author(s)
      堀居拓郎、小林遼平、田村大樹、森田純代、木村美香、畑田出穂
    • Organizer
      日本エピジェネティクス研究会
    • Place of Presentation
      奈良
    • Related Report
      2013 Research-status Report
  • [Presentation] 半数体ES細胞とCRISPR/Casを用いた高効率ゲノム編集2013

    • Author(s)
      堀居 拓郎,森田 純代,木村 美香,小林 遼平,田村 大樹,高橋 陵宇,木村 博信,末武 勲,大畑 広和, 岡本 康司,田嶋 正二,落谷 孝広,安部 由美子,畑田 出穂
    • Organizer
      ゲノム編集研究会
    • Place of Presentation
      東広島
    • Related Report
      2013 Research-status Report
  • [Presentation] 低酸素培養は初期胚や胎盤にDNAメチル化異常を引き起こす2012

    • Author(s)
      堀居拓郎、柳澤永吉、森田純代、木村美香、畑田出穂
    • Organizer
      第6回日本エピジェネティクス研究会年会
    • Place of Presentation
      東京
    • Related Report
      2012 Research-status Report
  • [Presentation] 初期胚の低酸素培養は初期胚や胎盤にDNAメチル化異常を引き起こす2012

    • Author(s)
      堀居拓郎、柳澤永吉、森田純代、木村美香、畑田出穂
    • Organizer
      第105回日本繁殖生物学会年会
    • Place of Presentation
      つくば
    • Related Report
      2012 Research-status Report
  • [Presentation] Risk of Epigenetic Changes is Increased by Low Oxygen in ART Embryo Cultures2012

    • Author(s)
      堀居拓郎、柳澤永吉、森田純代、木村美香、畑田出穂
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      博多
    • Related Report
      2012 Research-status Report
  • [Presentation] CRISPR/Cas9システムによる哺乳類半数体ES 細胞のゲノム操作

    • Author(s)
      堀居 拓郎,森田 純代,木村 美香,小林 遼平,田村 大樹,高橋 陵宇,木村 博信,末武 勲,大畑 広和, 岡本 康司,田嶋 正二,落谷 孝広,安部 由美子,畑田 出穂
    • Organizer
      日本分子生物学会
    • Place of Presentation
      神戸
    • Related Report
      2013 Research-status Report
  • [Presentation] 半数体胚由来2倍体ES 細胞(hddES)- 再生医療における新たな多能性幹細胞の選択肢

    • Author(s)
      小林 遼平,堀居 拓郎,田村 大樹,森田 純代,木村 美香,安部 由美子,畑田 出穂
    • Organizer
      日本分子生物学会
    • Place of Presentation
      神戸
    • Related Report
      2013 Research-status Report
  • [Presentation] CRISPR/Casと半数体ES細胞を用いたトリプルノックアウトおよび大規模染色体欠失の作製

    • Author(s)
      堀居 拓郎,森田 純代,木村 美香,高橋 陵宇,木村 博信,末武 勲,田嶋 正二, 落谷 孝広,畑田 出穂
    • Organizer
      日本繁殖生物学会
    • Place of Presentation
      東京
    • Related Report
      2013 Research-status Report
  • [Book] Epigenetic Instability in Embryonic Stem Cells2013

    • Author(s)
      T Horii and I Hatada
    • Total Pages
      16
    • Publisher
      INTECH
    • Related Report
      2012 Research-status Report
  • [Remarks] 半数体ES細胞およびCRISPRを用いた高効率ゲノム編集

    • URL

      http://epigenome.dept.showa.gunma-u.ac.jp/~hatada/index.php?id=42

    • Related Report
      2014 Annual Research Report
  • [Remarks] HOME/HATADA Laboratory

    • URL

      http://epigenome.dept.showa.gunma-u.ac.jp/~hatada/

    • Related Report
      2013 Research-status Report
  • [Remarks] 群馬大学 生体調節研究所附属 生体情報ゲノムリソースセンター

    • URL

      http://epigenome.dept.showa.gunma-u.ac.jp/~hatada/

    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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