Establishment of pluripotent stem cells with low tumorigenesity by use of genomic imprinting
Project/Area Number |
24613002
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
epigenetics
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Research Institution | Gunma University |
Principal Investigator |
HORII Takuro 群馬大学, 生体調節研究所, 助教 (00361387)
|
Co-Investigator(Kenkyū-buntansha) |
HATADA Izuho 群馬大学, 生体調節研究所, 教授 (50212147)
|
Project Period (FY) |
2012-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | 単為発生 / ゲノムインプリント / 腫瘍形成 / テラトーマ / 移植医療 / ES細胞 / インプリント遺伝子 / 単為発生胚 / 腫瘍 / 再生医療 |
Outline of Final Research Achievements |
The objective of this study is how reduce tumor formation after transplantation of ES/iPS-derived tissues. Our study has shown that parthenogenetic ES (PgES) cells are more suitable than normal diploid ES cells in the point of tumorigenesis after transplantation. Detailed analyses of DNA methylation and gene expression clarified that Snrpn gene causes tumor formation. Profiling of DNA methylation and gene expression before transplantation of ES/iPS-derived tissues will bring more safety transplantation therapy.
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Report
(4 results)
Research Products
(16 results)
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[Journal Article] Genome engineering of mammalian haploid embryonic stem cells using the Cas9/RNA system2013
Author(s)
Horii T, Morita S, Kimura M, Kobayashi R, Tamura D, Takahashi RU, Kimura H, Suetake I, Ohata H, Okamoto K, Tajima S, Ochiya T, Abe Y, Hatada I
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Journal Title
PeerJ
Volume: 1
Pages: e230-e230
DOI
Related Report
Peer Reviewed
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[Journal Article] Genome-Wide Analysis of DNA Methylation and Expression of MicroRNAs in Breast Cancer Cells.2012
Author(s)
Morita S, Takahashi RU, Yamashita R, Toyoda A, Horii T, Kimura M, Fujiyama A, Nakai K, Tajima S, Matoba R, Ochiya T, Hatada I.
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Journal Title
Int J Mol Sci.
Volume: 13
Pages: 8259-8272
Related Report
Peer Reviewed
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